An interventional study of calcium ionophore in IVF, Infertility and Artificial Oocyte Activation, sponsored by Chang Gung Memorial Hospital. Completed at 1 site in Taiwan. Open to female participants aged 20 Years to 50 Years. Per ClinicalTrials.gov, last updated 2026-03-20.
Sponsored by Chang Gung Memorial Hospital · Not applicable, Interventional, and Treatment
GM508 CultActive is a bicarbonate buffered reagent and ready-to-use designed to investigate oocytes of patients with failed fertilization after previous Intracytoplasmatic Sperm Injection cycles. The investigators hypothesize that GM508 CultActive will improve the fertilization rate and embryo development of such IVF patients (a history of poor fertilization, a history of poor embryo quality, and over 40 years old expected challenging to conceive).
2,506 studies on the registry are indexed under Infertility; 407 are open to participants now.
This study's enrollment of 68 is below the median of 120 across 1,698 interventional studies indexed under Infertility.
Browse Infertility studies →Chang Gung Memorial Hospital is the lead sponsor of 1,064 studies on the registry; 235 are open to participants now.
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Exclusion Criteria:
Oocytes treated with a ready-to-use ionophore compound (GM508 CultActive, Gynemed) within 15 min after ICSI (or 18 min in IVF). After 15 min of incubation in GM508 CultActive and a thorough washing process, the oocytes subjected to AOA will transfer to the same time-lapse culture dish with the controlled sibling group.
Other: calcium ionophore
Untreated control oocytes will immediately place into the time-lapse imaging system, and the culture protocol was routine.
The intervention group will be treated with a ready-to-use ionophore medium (GM508 CultActive, Gynemed) within 15 min after ICSI (or 18 min in IVF) based on the situation of sperm. After 15 min of incubation in GM508 CultActive and a thorough washing process, the oocytes will be cultured in the same protocol as the control group.
Also known as: GM508 CultActive
fertilization rate
The normal fertilization rate will defined as the number of zygotes with 2PN and 2PB per the total number of mature oocytes
Time frame: Day 1
blastocyst formation rate
NO. of blastocysts per 2PN zygotes
Time frame: Day 5
top-quality blastocyst formation rate
No. of 3AA, 4AA, 5AA or 6AA blastocysts / 2PN zygotes
Time frame: Day 5
pregnancy outcome
pregnancy rates, abortion rate, live birth rates,congenital anomaly rate
Time frame: 1 year
Plan to share: No
No publications or documents are linked to this record.
This study is completed, as verified in Mar 2026. You cannot join it, but the record below documents what was studied.
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Chang Gung Memorial Hospital