An interventional study of Vibration in Vibration; Exposure and Muscle Physiology, sponsored by Istanbul Physical Medicine Rehabilitation Training and Research Hospital. Completed at 1 site in Turkey. Open to male participants aged 20 Years to 45 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2022-10-13.
Sponsored by Istanbul Physical Medicine Rehabilitation Training and Research Hospital · Not applicable, Interventional, and Basic science
Whole body vibration (WBV) is a therapeutic modality in the form of exercise on a vibrating platform with an amplitude of 2-4 mm at a frequency of 25-50 Hz, which is used with increased popularity in sports medicine and rehabilitation due to its beneficial effects on muscle strength, balance, postural control, bone formation, and circulation. Beneficial effects on muscle strength and athletic performance have been reported. Spinal reflexes explain these beneficial neuromuscular effects. However, the neuronal circuit and receptors of the reflex response have not been defined precisely. A group of researchers propose that the reflex system underlying the neuromuscular effects of WBV is the Tonic vibration reflex (TVR), whose receptor is the muscle spindle; other researchers claim that the reflex latency induced by WBV is 4-5 ms longer than the TVR latency, so it is a bone myoregulation reflex whose receptor is osteocytes.
The muscle spindle has sympathetic innervation. It has been reported that in case of increased sympathetic activity, muscle spindle sensitivity may increase and short-latency stretch reflex may be facilitated. The variation of muscle spindle activity with sympathetic activity may provide an opportunity to define the nature of the reflex response during WBV. Muscle spindles are more sensitive to vibrations around 100 Hz. This study has two hypotheses: According to the first hypothesis, WBV activates muscle spindles and the reflex latency induced by WBV is the same as TVR latency, and the latency does not change with increased sympathetic activity. According to the alternative hypothesis, WBV activates osteocytes, and WBV-induced reflex latency is longer than TVR latency. With increased sympathetic activity, the WBV reflex becomes dominant and the WBV-induced reflex latency becomes shorter. The aim of this research is to determine which of these two hypotheses is valid.
22 healthy males between the ages of 20 and 45 will be recruited for this study. All subjects will receive pre-study informed consent. All experimental procedures were designed with the Helsinki declaration in mind and approved by the local ethics committee. In all subjects, whole body vibration(WBV) and isolated tendon vibration will be applied before, after, and during sympathetic activation maneuvers. Reflex responses induced by WBV and isolated tendon vibration will be recorded by surface electromyography (EMG) from the right soleus muscle. Latency calculation will be made using the "Cumulative averaging method".
Mental arithmetic task and cold stress test will be applied as sympathetic activation maneuver. For the mental arithmetic task, the subject is asked to mentally subtract a two-digit number from a four-digit number and respond within five seconds. This arithmetic calculation is repeated for different numbers. This test is continued for two minutes. For the cold stimulation test, subjects are asked to dip their right hand into 2-4 degrees cold water up to the elbow and hold it in the water for two minutes. At this time, either WBV or tendon vibration will be applied. After a ten-minute break, a cold stimulation test will be applied to the left hand and the subject will be asked to do mental arithmetic calculations simultaneously. Meanwhile, other vibrations will be used. The sympathetic response will be evaluated by heart rate and sympathetic skin response.
Whole body vibration will be applied to the right leg while subjects are sitting with their knee, hip, and ankle joints in a 90-degree position.
The left leg will rest on a vibration-isolated platform. During this position, he will be asked not to voluntarily contract the calf muscle. The vibration will be applied with the PowerPlate® Pro5 WBV (London UK) device. The vibration amplitude will be 2.2 mm. The vibration frequency will be 30, 35, 40, and 45 Hz. Each vibration frequency will be applied for 30 seconds. A 5-second rest period will be applied between 30-second vibrations. Piezo-electric accelerometer (LIS344ALH, full-scale of ± 6 g, linear accelerometer, ECOPACK®, Mansfield, TX, USA) will be fixed on the WBV device platform for reflex latency calculation. For tonic vibration 100, 135, 150, and 180 Hz vibrations will be applied to the Achilles tendon. 30 seconds of vibration will be applied at each frequency. A direct current (DC) motor vibrator will be used to apply this vibration (Bosch™, amplitude 2 mm, motor speed 5000-20000 / min). A piezo-electric accelerometer (LIS344ALH, full-scale of ± 6 g, linear accelerometer, ECOPACK®, Mansfield, TX, USA) will be placed on the skin of the Achilles tendon to calculate the tonic vibration reflex latency. Superficial EMG recordings will be taken from the right soleus muscle with the bipolar technique. A pair of self-adhesive Ag/AgCl (KENDALL® Coviden, Massachusetts, USA) electrodes will be attached to the skin. The electrodes on the soleus will be positioned according to the SENIAM protocol. The ground electrode will have adhered to the right lateral malleolus. Before the electrodes are attached to the skin, if there are hairs on the skin, they will be shaved and the oil layer on the skin will be cleaned with alcohol cotton. In order to increase the moisture of the skin, ECG gel will be applied to the skin area where the electrode will be attached. Superficial EMG recordings will be taken with a PowerLab ® (ADInstrument ADInstruments, Oxford, UK) data recorder with a sample rate of 40 kHz.
EMG recordings will be analyzed offline with LabChart7 Pro® version 7.3.8 (ADInstrument, Oxford, UK). The acceleration measurement data will be recorded with the PowerLab (ADInstrument London) data logger simultaneously with the EMG recording. Acceleration recording will be made with a sample rate of 40 kHz. Simultaneously, heart rate and sympathetic skin response will be captured with a Biopac (Biopac System Inc CA) data recorder at a rate of 20 kHz.
Istanbul Physical Medicine Rehabilitation Training and Research Hospital is the lead sponsor of 119 studies on the registry; 27 are open to participants now.
Counted across the registry records on this site, refreshed daily.
Exclusion Criteria:
Vibration (WBV, and tendon vibration) and sympathetic activation maneuvers will be applied to participants
Procedure: Vibration
whole-body vibration, and tendon vibrations will be applied to the human body
Also known as: whole body vibration, tendon vibration, sympathetic activation maneuver
Whole-body vibration (WBV) induced reflex latency
Latency of the soleus reflex response induced by WBV. Its unit is milliseconds
Time frame: during intervention, an average of 3 minute
Tendon vibration induced reflex latency
Vibration will be applied to the achilles tendon. The soleus reflex latency induced by the tendon vibration will be measured. Its unit is milliseconds
Time frame: during intervention, an average of 3 minute
Heart rate variability
Heart rate variability will be measured to determine sympathetic activity. Low-frequency (LF) and high-frequency (HF) were considered to reflect sympathetic and parasympathetic activity, respectively. The autonomic balance was obtained by the ratio between the sympathetic and parasympathetic areas (LF/HF); wherein ratios \>1, \<1, and 1 were indicative of sympatheticotonia, vagotonia, and sympathovagal balance, respectively.
Time frame: during intervention, an average of 2 hours
Sympathetic skin response
Sympathetic skin response will be measured to determine sympathetic activity.Unit is milliVolt
Time frame: during intervention, an average of 2 hours
This study is completed, as verified in Oct 2022. You cannot join it, but the record below documents what was studied.
Get an email when the registry record changes — status, dates, results — or when someone posts here.
Sign in to followQuestions and observations about this study, from anyone following it. Not medical advice, and not a channel to the study team — their contact details are on the registry record.
Sign in to join the discussion. Reading takes no account; posting does. You choose a display name, and a pseudonym is the default.
Nothing here yet. If you are running this trial, taking part in it, or weighing whether to, this is the place to say so.
Istanbul Physical Medicine Rehabilitation Training and Research Hospital