Booster Dose Study to Assess the Safety and Immunogenicity of ACM-001 Administered Intramuscularly or Intranasally.

1. Signed informed consent prior to any study-related procedure;
2. Subjects must have received a complete primary vaccination schedule and a third and/or fourth booster dose with registered and commercial vaccine(s) against SARS-CoV-2, of which the last dose was given at least 3 months prior to study vaccination (maximum of 1,000 IU of anti-S IgG);
3. Healthy males and females, 18-55 years of age, inclusive at screening;
4. Body mass index (BMI) ≥ 18.0 and \< 30.0 kg/m2;
5. Good health, based upon the results of medical history, physical examination, vital signs, laboratory profiles of both blood and urine, and according to the clinical judgement of the investigator;
6. Female participants of childbearing potential must be willing to comply with effective contraception up to 90 days after the study vaccine administration;
7. Willing to comply with the study procedures.

Exclusion Criteria:

• 1. Known immune deficiency; 2. Chronic airway disease; 3. Has experienced an acute illness, as determined by the investigator, or fever (>38.5°C) within 72 hours prior to study vaccine administration; in such case, the subject may be screened again after normalization of the temperature and/or healing of the illness; 4. Active hay fever or other active allergies involving the lower airways (bronchial and pulmonary); 5. Laboratory-confirmed PCR positive result for SARS-CoV-2 in nose/throat swab during screening; 6. Previous participation in a study to evaluate a non-registered COVID-19 vaccine within 3 months prior to study vaccination; 7. Received any other commercial vaccine within the 28 days prior to enrolment in the study, or immunization planned within 3 months after enrolment in the study (influenza vaccines are allowed up to one week before and one week after study vaccination; Exclusion criteria CONFIDENTIAL Cohort 2: 15 μg Protein (N=10), IN Cohort 4: 5 μg Protein, 25 μg CpG (N=10), IN Cohort 6: 15 μg Protein, 25 μg CpG (N=10), IN Cohort 8: 15 μg Protein, 125 μg CpG (N=10), IN ACM-001-01 Version 2.0 09 May 2022 Page 10 of 74 DocuSign Envelope ID: C34D91C3-4686-427D-BB78-CF7178216E74 CONFIDENTIAL 8. Any confirmed severe allergic reactions (urticaria, angioedema or anaphylaxis); 9. Evidence of any other active or chronic disease (hematologic, renal, hepatic, cardiovascular, neurologic, endocrinal, gastrointestinal, oncologic, pulmonary, immunologic or psychiatric disorders) or condition that could interfere with, or for which the treatment of might interfere with the conduct of the study, or that would pose an unacceptable risk to the subject in the opinion of the investigator (following a detailed medical history, physical examination, vital signs (systolic and diastolic blood pressure, and body temperature). Minor deviations from the normal range may be accepted, if judged without clinical relevance by the Investigator; 10. Clinicallysignificantabnormalities,asjudgedbytheInvestigator,in laboratory test results (including blood chemistry, hematology and urinalysis). In the case of uncertain or questionable results, tests performed during screening may be repeated before randomization to confirm eligibility or judged to be clinically irrelevant for healthy subjects; 11. Positive hepatitis B surface antigen, hepatitis C antibody, or human immunodeficiency virus antibody at screening; 12. Asplenia; 13. Useofanychronictreatmentwithsystemiccorticosteroids(episodic treatments with topical and intranasal corticosteroids are allowed) and immunosuppressive drugs; 14. Use of paracetamol or non-steroidal anti-inflammatory drugs (NSAIDs) within 72 hours prior to vaccination; 15. Receivedbloodproducts(transfusionsorimmunoglobulins)within3 months prior to screening, or planned administration of blood products or immunoglobulins during the study; 16. History of substance use disorder (alcohol, illegal substances), current alcohol use disorder (according to Australian guidelines: https://www.health.gov.au/news/australian-alcohol-guidelines- revised) or drug abuse; 17. Participation in an investigational drug or device study within 3 months prior to first study vaccine administration or more than 4 times a year; 18. Lossordonationofbloodover500mLwithin3months(males)or4 months (females) prior to screening or intention to donate blood or blood products during the study; 19. History of bleeding disorder (e.g., factor deficiency, coagulopathy, or platelet disorder requiring special precautions), significant bleeding or bruising following IM injections or venous punctures, or currently receiving anticoagulants; 20. Has body art (e.g., tattoos), skin lesions or abnormalities that could interfere with the observation of injection site reactions; ACM-001-01 Version 2.0 09 May 2022 Page 11 of 74

DocuSign Envelope ID: C34D91C3-4686-427D-BB78-CF7178216E74 Endpoints 21. Close contact with laboratory-confirmed COVID-19 cases within 10 days prior to vaccination, high risk of exposure or has an occupation with a high risk of exposure to SARS-CoV-2 (emergency response); 22. Pregnancy confirmed by a positive pregnancy test, lactation or intention to become pregnant during the study; 23. Any cancer diagnosed and/or treated within the past 5 years (except basal cell carcinoma of the skin and cervical carcinoma in situ); 24. Veins not suitable for repeated blood sampling; 25. Serious reaction, such as anaphylactic reaction, following primary COVID-19 vaccination; 26. Any known factor, condition, or disease that might interfere with treatment compliance, study conduct or interpretation of the results; 27. Sponsor employees or Investigator site personnel directly affiliated with this study, and their immediate families. Immediate family is defined as a spouse, parent, child or sibling, whether biological or legally adopted, including children of newly composed families.