An interventional study of Low intensity exercise session and Moderate intensity exercise session in Cellular Responses Within Skeletal Muscle in Response to Exercise of Different Intensities, Relationship Between the Cellular Responses in Skeletal Muscle and the Changes in the Metabolomic Profile and Changes Within Adipose Tissue in Response to Exercise at the Three Different Intensities, sponsored by University of Michigan. Completed at 1 site in United States. Open to participants aged 18 Years to 40 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2024-07-26.
Sponsored by University of Michigan · Not applicable, Interventional, and Basic science
The study will assess the exercise-induced changes in chromatin accessibility and gene expression in a cell type-specific manner in skeletal muscle from healthy adults in response to exercise at three different intensities - 1) low-intensity continuous exercise, 2) moderate-intensity continuous exercise, and 3) high-intensity interval exercise.
University of Michigan is the lead sponsor of 1,475 studies on the registry; 196 are open to participants now.
Of its 162 completed or terminated interventional studies of FDA-regulated products, 128 (79%) have results posted.
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Exclusion Criteria:
subjects will exercise at 30% maximal oxygen consumption (VO2max) for 45-60 minutes
Behavioral: Low intensity exercise session
subjects will exercise at 65% maximal oxygen consumption (VO2max) for 30-45 minutes
Behavioral: Moderate intensity exercise session
subjects will completed 10x1minute exercise intervals at maximal oxygen consumption (VO2max) with 1 minute active recovery between
Behavioral: High intensity exercise session
subjects will exercise at 30% maximal oxygen consumption (VO2max) for 45-60 minutes
subjects will exercise at 65% maximal oxygen consumption (VO2max) for 30-45 minutes
subjects will completed 10x1minute exercise intervals at maximal oxygen consumption (VO2max) with 1 minute active recovery between
post-translational modifications of skeletal muscle oxidative enzymes
Western blot
Time frame: 30 minutes before exercise
post-translational modifications of skeletal muscle oxidative enzymes
Western blot
Time frame: immediately after exercise
post-translational modifications of skeletal muscle oxidative enzymes
Western blot
Time frame: 60 minutes after exercise
post-translational modifications of Adipose tissue metabolic proteins
Western blot
Time frame: 15 minutes before exercise
post-translational modifications of Adipose tissue metabolic proteins
Western blot
Time frame: 75 minutes after exercise
mRNA expression of skeletal muscle oxidative enzymes
qPCR
Time frame: 30 minutes before exercise
mRNA expression of skeletal muscle oxidative enzymes
qPCR
Time frame: immediately after exercise
mRNA expression of skeletal muscle oxidative enzymes
qPCR
Time frame: 60 minutes after exercise
mRNA expression of adipose tissue metabolic enzymes
qPCR
Time frame: 15 minutes before exercise
mRNA expression of adipose tissue metabolic enzymes
qPCR
Time frame: 75 minutes after exercise
This study is completed, as verified in Jul 2024. You cannot join it, but the record below documents what was studied.
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