A Phase 1/2 interventional study of Isothiocyanate rich sprouts and Placebo Juice in Metabolic Disturbance, sponsored by The Swedish School of Sport and Health Sciences. Status unknown at 1 site in Sweden. Open to participants aged 18 Years to 45 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2022-12-06.
Sponsored by The Swedish School of Sport and Health Sciences · Phase 1/2, Interventional, and Prevention
Many sprouts, for example broccoli sprouts, contain substances that have shown to have protective effects, both against oxidative stress and against carcinogenic substances.
In this study we will investigate the activation of the body's antioxidant defense system and effect on blood lactate and glucose after 1 week of isothiocyanate rich sprout supplementation. The subjects will perform maximal and submaximal work test, blood sampling, muscle biopsies and blood pressure monitoring post supplementation. We hypothesize that 1 week of supplementation of isothiocyanate rich sprouts alters blood lactate and glucose metabolism and activates the body's antioxidant defense system.
Many sprouts, for example broccoli sprouts, contain high levels of glucosinolates, which can be converted to the bioactive substances isothiocyanates. This conversion is assisted by another plant enzyme, myrosinase, which is released when the plant is mechanically processed. It is believed that this reaction is part of the plant's natural defense against pest insects and other animals that attack the plant in the early developmental stage. In cell and animal studies supplementation with isothiocyanates has been shown to induce the protein Nrf2 which is a so-called "Master regulator" of the body's antioxidant defenses and activates more than 200 genes that together provide a strong protection against oxidative and mutagenic stress.
In this study we will investigate Nrf2 activation in human skeletal muscle and effect on lactate-, glucose metabolism after 1 week of isothiocyanate rich sprout supplementation. The study is a double-blinded, randomized, placebo controlled, cross-over study with 4 weeks wash-out between the interventions. We will perform maximal- and submaximal work test and monitor blood glucose and lactate during the tests. Also, in the post intervention phases we will perform muscle biopsies, glucose tolerance tests and monitor blood pressure.
The hypothesis is that 1 week of isothiocyanate rich sprout supplementation activates Nrf2 in human skeletal muscle and alters blood lactate and glucose metabolism in rest and during exercise.
The Swedish School of Sport and Health Sciences is the lead sponsor of 12 studies on the registry; 6 are open to participants now.
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Exclusion Criteria:
The juices will contain 37,5g isothiocyanate rich sprouts and be delivered frozen to the subjects. The subjects will be instructed to consume two juices per day.
Dietary Supplement: Isothiocyanate rich sprouts
The juices will contain 37,5g alfalfa sprouts and be delivered frozen to the subjects. The subjects will be instructed to consume two juices per day.
Dietary Supplement: Placebo Juice
Isothiocyanate rich sprout juice consumption for 1 week
Sprout juice devoid of isothiocyanates for 1 week
Blood lactate
Changes in blood lactate during exercise
Time frame: Acute during sub maximal exercise
Nrf2 activation
Increased Nrf2 protein in human skeletal muscle
Time frame: Acute after 1 week of supplementation
Glucose regulation
Improved continuous glucose control
Time frame: Throughout 1 week of supplementation
Glucose tolerance test
Improved glucose regulation in rest
Time frame: 120 minutes
Clinic blood pressure
Blood pressure regulation
Time frame: Acute after 1 week of supplementation
Plan to share: No
No publications or documents are linked to this record.
This study is status unknown, as verified in Dec 2022. You cannot join it, but the record below documents what was studied.
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The Swedish School of Sport and Health Sciences