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CompletedNCT04594122SFFF CambodiaUpdated Sep 2, 2021

Good Hygiene Practice Interventions for Safer Pork at Traditional Markets in Cambodia

An interventional study of Improve hygiene practice of pork sellers in Food Borne Diseases and Food Safety, sponsored by The International Livestock Research Institute (ILRI). Completed at 1 site in Cambodia. Open to participants aged 18 Years to 65 Years. Per ClinicalTrials.gov, last updated 2021-09-02.

Sponsored by The International Livestock Research Institute (ILRI) · Not applicable, Interventional, and Other

Phase
Not applicable
Study type
Interventional
Enrollment
360
Allocation
Randomized
Ages
18 Years to 65 Years
Sex
All
01

Study summary

Safe Food Fair Food for Cambodia (SFFF) is a Feed the Future Innovation Lab project funded by USAID. This 3-year project aims to improve food safety of animal source food (ASF) in Cambodia. SFFF was awarded to the International Livestock Research Institute (ILRI), National Animal Health and Production Research Institute (NAHPRI) and Livestock Development for Community Livelihood Organization (LDC) and in collaboration with Cambodian Ministry of Health CDC and NIPH. Based on the findings and consultations with food safety stakeholders in Cambodia, we have developed a set of interventions to improve hygienic practice and pork safety at the traditional markets in Cambodia. Those interventions will be introduced and tested at retail in six selected provinces using Randomized Controlled Trials (RCT). To ensure compliance of targeted actors (e.g. retailers), participatory methods (e.g. FGD) were used to validate intervention packages. There are two steps of intervention which contain of part 1) Retailer formative research for SFFF Cambodia and 2) Interventions for Good Hygiene Practices for Safer Pork at Traditional Markets.

Part 1. Retailer formative research for SFFF Cambodia: We will implement an intervention package to this trial group and collect biological sampling to determine the hygienic status before and after the intervention. The intervention packages will be developed for SFFF Cambodia in consultation with partners and based on findings of SFFF Cambodia project.

Part 2. Intervention for Good Hygiene Practices for Safer Pork at Traditional Markets: A randomized controlled trial (RCT) intervention will be conducted in 12 markets in 6 provinces. Those provinces were selected based on the prevalence of Salmonella in a market survey study, namely Kampot, Kampong Cham, Kampong Speu, Takeo, Siem Reap, and Phnom Penh. Another 12 markets, in the same provinces, will be used as a control group. In total 24 traditional wet markets will be included in the sampling, by selecting the 4 largest traditional markets in the six provinces with at least 15 pork shops. At each market, 15 pork shops were selected for sampling. The intervention package includes 5 keys actions (Handbook) and provision of equipment incentive (e.g. inox tray, easy-clean surface material), and training on good hygiene practices.

Read the detailed description
  1. Selection of provinces, markets and retailers

    Six provinces were selected including Phnom Penh, Siem Reap, Takeo, Kampong Cham, Kampot, Kampong Speu with the following selection criteria: i) The number of markets in provinces, ii) Salmonella prevalence (above 50%), iii) Population density and risk, urban - rural, iv) Distance from laboratory, v) Compliance of market board and traders, local animal health workers. Phnom Penh had a reported Salmonella prevalence of less than 50% in the recent market study but was included as an urban center with expected high output of pork sells. In each province, four markets which have at least 15 pork shops will be selected. Among those markets, two markets will be assigned for control and trial groups.

    Group of retailers will be chosen from selected markets for the intervention based on their "similarity" (e.g., environment, shop facilities, sells volume) and their compliance to participate. Intervention package includes providing incentive equipment and training on good hygiene practice. Equipment provision comprise: (i) equip the shops with inox trays and advise them to separate raw pork/processed pork and intestines; (ii) In case the surface of the shop is not made from granite or inox, we will provide them with feasible surface cover material; (iii) Apron to retailers with the project logo/brand; (iv)Shop's banner with our logo; (v) Hand washing soap (antibacterial) and dish detergent; (vi) Disinfectant spray (Anolyte).

    Guide retailers to rearrange their shops to suit their own facility and space. Introduce plastic cutting boards for light cut, check currently used wooden cutting boards at shop to advice to use properly. Introduce frequent washing and disinfection of sale place and shop equipment. Washing with dish detergent and disinfection using spray disinfectant (Electrochemically activated water, pH=7). This shall include:

    • Cutting boards. Washing with dish detergent (soap) and water with scrub, then disinfection using spraying bleach. Leave for five minutes then rinse with water (can also be a spray) and let it air dry. At least prior to open or after closing the shop and once in between.
    • Knives. At least prior to open or after closing the shop and once in between.
    • Cloth. Guide how to use cloth. Each shop should have 2-3 cloth to wipe separate surfaces/tools.
    • Table surface. Require retailers to wash and disinfect throughout at the end of selling day; and apply disinfectant spray at the beginning of the selling day. Only retailers with an easy to clean surface (inox, granite or plastic etc.) shall be selected.
    • Training. Introduce frequent washing of hands, at least once within each selling hour, with clean water and liquid soap, and dry by tissue paper. Proper disposal of tissue paper in closed top bin

    For control group: No intervention or training activities for this group but collection of biological samples and observation checklist to determine hygienic status at their shops over three consecutive days.

  2. Intervention description

    1. Procedure to introduce the intervention:

      • Introduce and discuss trial with market authorities and vets (as appropriate)
      • Identify potential retailers, discuss and make plan with them
      • Pre- evaluate current knowledge and practice of selected retailers.
      • Procure suitable (shop size) equipment for "trial" retailers
      • Provide training, instructions and guidelines for selected retailers and introduce equipment
      • Monitoring of hygienic practice, sampling and checking practice of retailer
      • Timelines: "Training and equipment" in the 1st week, follow up, and supervise in week 2-4, sampling will be conducted in the fourth week.
      • Summary report, sharing and discussion with trial retailers to re-evaluate their knowledge.
    2. Procedure for sampling and analysis:

      • At each market, 15 of pork vendors will be selected for sampling.
      • From each vendor, one specimen will be purchased approximately 300 to 400 grams each.
      • All pork samples (n=360) from trial and control groups will be tested for total bacteria count (TBC).
      • In addition, a sub-set of 60 cut pork samples of trial group will be also analyzed for Salmonella Yes/No (qualitative), 30 out of 60 of these samples will be quantified for Salmonella concentration using MPN method.
02

Conditions studied

  • Food Borne Diseases
  • Food Safety

Keywords

  • Hygiene Practice
  • Bacterial Contamination
  • Traditional Market
  • Pork Shop
  • Cambodia
03

In context

Lead sponsor

The International Livestock Research Institute (ILRI) is the lead sponsor of 3 studies on the registry; none are open to participants now.

Counted across the registry records on this site, refreshed daily.

04

Who can participate

Ages eligible
18 Years to 65 Years
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  • Under selected markets
  • Selling pork only
  • Daily selling
  • Voluntary

Exclusion criteria

Exclusion Criteria:

  • Out of the age range 18-65
05

Study design

Phase
Not applicable
Primary purpose
Other
Allocation
Randomized
Intervention model
Parallel assignment
Masking
None (open label)
Enrollment
360 participants (actual)

Study arms

  • No intervention
    Control

    No intervention in this group

  • Experimental
    Intervention

    Hygiene package, training, branding, and certification

    Behavioral: Improve hygiene practice of pork sellers

Interventions

  • BehavioralImprove hygiene practice of pork sellers

    includes 5 keys actions (Handbook) and provision of equipment incentive and training on good hygiene practices, branding, and certification

06

What researchers measure

Primary outcomes

  1. Proportion of samples fulfill Cambodian microbiological standards for pork

    Total bacterial count in pork samples will be measured from both trial and control groups to assess level of compliance with national standards

    Time frame: 16 weeks

Secondary outcomes

  1. Change in knowledge of pork retailers

    There are 8 questions on the knowledge part. Each correct answer is given 1 score. The minimum and maximum scores of each participant are 0 and 8, respectively. The means of knowledge score will be compared to see the difference between the 2 groups.

    Time frame: 16 weeks

  2. Change in practice of pork retailers

    The practice will be observed using a checklist during the selling time of the sampling day. There are 20 items on the checklist. Each appropriate practice is given 1 score per item. Practice score will be ranged from 0 to 20. The difference between the 2 groups will be compared using means of practice score.

    Time frame: 16 weeks

07

Study locations

1 site
  • National Animal Health and Production Research Institute
    Phnom Penh, Cambodia
08

References and documents

Publications

  • Dang-Xuan S, Nguyen-Viet H, Unger F, Pham-Duc P, Grace D, Tran-Thi N, Barot M, Pham-Thi N, Makita K. Quantitative risk assessment of human salmonellosis in the smallholder pig value chains in urban of Vietnam. Int J Public Health. 2017 Feb;62(Suppl 1):93-102. doi: 10.1007/s00038-016-0921-x. Epub 2016 Nov 11. PubMed 27837223 ↗
  • Darapheak C, Takano T, Kizuki M, Nakamura K, Seino K. Consumption of animal source foods and dietary diversity reduce stunting in children in Cambodia. Int Arch Med. 2013 Jul 17;6:29. doi: 10.1186/1755-7682-6-29. eCollection 2013. PubMed 23866682 ↗
  • Devleesschauwer B, Haagsma JA, Angulo FJ, Bellinger DC, Cole D, Dopfer D, Fazil A, Fevre EM, Gibb HJ, Hald T, Kirk MD, Lake RJ, Maertens de Noordhout C, Mathers CD, McDonald SA, Pires SM, Speybroeck N, Thomas MK, Torgerson PR, Wu F, Havelaar AH, Praet N. Methodological Framework for World Health Organization Estimates of the Global Burden of Foodborne Disease. PLoS One. 2015 Dec 3;10(12):e0142498. doi: 10.1371/journal.pone.0142498. eCollection 2015. PubMed 26633883 ↗
  • Dror DK, Allen LH. The importance of milk and other animal-source foods for children in low-income countries. Food Nutr Bull. 2011 Sep;32(3):227-43. doi: 10.1177/156482651103200307. PubMed 22073797 ↗
  • Gilbert M, Conchedda G, Van Boeckel TP, Cinardi G, Linard C, Nicolas G, Thanapongtharm W, D'Aietti L, Wint W, Newman SH, Robinson TP. Income Disparities and the Global Distribution of Intensively Farmed Chicken and Pigs. PLoS One. 2015 Jul 31;10(7):e0133381. doi: 10.1371/journal.pone.0133381. eCollection 2015. PubMed 26230336 ↗
  • Grace D. Food Safety in Low and Middle Income Countries. Int J Environ Res Public Health. 2015 Aug 27;12(9):10490-507. doi: 10.3390/ijerph120910490. PubMed 26343693 ↗
  • Grace D, Omore A, Randolph T, Kang'ethe E, Nasinyama GW, Mohammed HO. Risk assessment for Escherichia coli O157:H7 in marketed unpasteurized milk in selected East African countries. J Food Prot. 2008 Feb;71(2):257-63. doi: 10.4315/0362-028x-71.2.257. PubMed 18326173 ↗
  • Grace D, Dipeolu M, Olawoye J, Ojo E, Odebode S, Agbaje M, Akindana G, Randolph T. Evaluating a group-based intervention to improve the safety of meat in Bodija market, Ibadan, Nigeria. Trop Anim Health Prod. 2012 Sep;44 Suppl 1:S61-6. doi: 10.1007/s11250-012-0208-z. Epub 2012 Aug 7. PubMed 22869336 ↗
  • Grace D, Olowoye J, Dipeolu M, Odebode S, Randolph T. The influence of gender and group membership on food safety: the case of meat sellers in Bodija market, Ibadan, Nigeria. Trop Anim Health Prod. 2012 Sep;44 Suppl 1:S53-9. doi: 10.1007/s11250-012-0207-0. Epub 2012 Aug 8. PubMed 22872520 ↗
  • Hoang VM, Tran TA, Ha AD, Nguyen VH. Cost of Hospitalization for Foodborne Diarrhea: A Case Study from Vietnam. J Korean Med Sci. 2015 Nov;30 Suppl 2(Suppl 2):S178-82. doi: 10.3346/jkms.2015.30.S2.S178. Epub 2015 Nov 6. PubMed 26617452 ↗
  • Kouame-Sina SM, Makita K, Costard S, Grace D, Dadie A, Dje M, Bonfoh B. Hazard identification and exposure assessment for bacterial risk assessment of informally marketed milk in Abidjan, Cote d'Ivoire. Food Nutr Bull. 2012 Dec;33(4):223-34. doi: 10.1177/156482651203300402. Erratum In: Food Nutr Bull. 2013 Mar;34(1):120. PubMed 23424888 ↗
  • Kruy SL, Soares JL, Ping S, Sainte-Marie FF. [Microbiological quality of " ice, ice cream. sorbet" sold on the streets of Phnom Penh; April 1996-April 1997]. Bull Soc Pathol Exot. 2001 Dec;94(5):411-4. French. PubMed 11889944 ↗
  • Lay KS, Vuthy Y, Song P, Phol K, Sarthou JL. Prevalence, numbers and antimicrobial susceptibilities of Salmonella serovars and Campylobacter spp. in retail poultry in Phnom Penh, Cambodia. J Vet Med Sci. 2011 Mar;73(3):325-9. doi: 10.1292/jvms.10-0373. Epub 2010 Nov 2. PubMed 21060246 ↗
  • Makita K, Fevre EM, Waiswa C, Eisler MC, Welburn SC. How human brucellosis incidence in urban Kampala can be reduced most efficiently? A stochastic risk assessment of informally-marketed milk. PLoS One. 2010 Dec 1;5(12):e14188. doi: 10.1371/journal.pone.0014188. PubMed 21152072 ↗
  • Meng CY, Smith BL, Bodhidatta L, Richard SA, Vansith K, Thy B, Srijan A, Serichantalergs O, Mason CJ. Etiology of diarrhea in young children and patterns of antibiotic resistance in Cambodia. Pediatr Infect Dis J. 2011 Apr;30(4):331-5. doi: 10.1097/INF.0b013e3181fb6f82. PubMed 21412204 ↗
  • Nguyen-Viet H, Tuyet-Hanh TT, Unger F, Dang-Xuan S, Grace D. Food safety in Vietnam: where we are at and what we can learn from international experiences. Infect Dis Poverty. 2017 Feb 16;6(1):39. doi: 10.1186/s40249-017-0249-7. PubMed 28209208 ↗
  • Osbjer K, Boqvist S, Sokerya S, Chheng K, San S, Davun H, Rautelin H, Magnusson U. Risk factors associated with Campylobacter detected by PCR in humans and animals in rural Cambodia. Epidemiol Infect. 2016 Oct;144(14):2979-2988. doi: 10.1017/S095026881600114X. Epub 2016 Jun 23. PubMed 27334412 ↗
  • Otte MJ, Gumm ID. Intra-cluster correlation coefficients of 20 infections calculated from the results of cluster-sample surveys. Prev Vet Med. 1997 Jul;31(1-2):147-50. doi: 10.1016/s0167-5877(96)01108-7. No abstract available. PubMed 9234433 ↗
  • Robinson TP, Wint GR, Conchedda G, Van Boeckel TP, Ercoli V, Palamara E, Cinardi G, D'Aietti L, Hay SI, Gilbert M. Mapping the global distribution of livestock. PLoS One. 2014 May 29;9(5):e96084. doi: 10.1371/journal.pone.0096084. eCollection 2014. PubMed 24875496 ↗
  • Ross T, Sumner J. A simple, spreadsheet-based, food safety risk assessment tool. Int J Food Microbiol. 2002 Jul 25;77(1-2):39-53. doi: 10.1016/s0168-1605(02)00061-2. PubMed 12076037 ↗
  • Slovic P. Perception of risk. Science. 1987 Apr 17;236(4799):280-5. doi: 10.1126/science.3563507. PubMed 3563507 ↗

Individual participant data

Plan to share: No — RCT data can be shared for other researchers but not the IPD.

09

Updates

Tracking since Sep 25, 2026
No changes since tracking began. The registry record was last updated on Sep 2, 2021, before this site started recording changes on Sep 25, 2026. Its history is on ClinicalTrials.gov ↗
10

Registry details

Key details

Study ID
NCT04594122
Lead sponsor
The International Livestock Research Institute (ILRI)
Collaborators
National Animal Health and Production Research Institute, Cambodia, Livestock Development for Community Livelihood Organization, Cambodia, Emory University
Responsible party
Sponsor
First posted
Oct 20, 2020
Start date
Jun 30, 2020
Primary completion
Feb 28, 2021
Completion
Mar 15, 2021
Last update
Sep 2, 2021

Study contacts

Delia Randolph, PhD
principal investigator · The International Livestock Research Institute (ILRI)

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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This study is completed, as verified in Oct 2020. You cannot join it, but the record below documents what was studied.

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