An interventional study of burn wound dressing in Burns and Burn Wound, sponsored by Izmir Katip Celebi University. Completed at 1 site in Turkey. Open to participants aged 8 Weeks to 12 Weeks, including healthy volunteers. Per ClinicalTrials.gov, last updated 2022-08-03.
Sponsored by Izmir Katip Celebi University · Not applicable, Interventional, and Treatment
This experimental study will be carried out at Ege University Laboratory Animals Application and Research Center to identify the efficacy of 10% propolis and 15% propolis on wound healing in a second-degree superficial burn wound.
In the study, 36 adult (8-12 week old) Wistar-Albino rats with a weight of 200-300 grams will be used in each group. Each group will include 6 rats. Stratified randomization will be used to randomize rats according to their gender and weight. After randomization, a burnt area will be created on the back of the rats and dressings will be made every day.
Following the creation of the burn, biopsy samples will be taken on the 3rd, 7th, 14th and 21st days and at the light microscopic level; bulla, erythema, oedema, burn depth, inflammatory cell infiltration, necrosis, ulceration, angiogenesis, neovascularization, fibroblast proliferation, collagenization, epithelization, fibrosis, the number of hair follicles and damage to the skin attachments.
In the immunohistochemical examination of biopsy samples, samples will be taken for the evaluation of superoxide dismutase, malondialdehyde, myeloperoxidase in the evaluation of the tumour necrosis factor-alpha, interleukin-1β and oxidative stress in the EBB period in order to evaluate the acute phase reactants. Fungal and gram staining will be done in order to determine the pathogen reproduction in biopsy samples taken on the 3rd and 7th day. Masson trichrome in order to detect fibrosis in biopsy samples taken on the 14th and 21st day; Hydroxyproline examination will be examined in order to evaluate collagen formation.
719 studies on the registry are indexed under Burns; 145 are open to participants now.
This study's enrollment of 36 is close to the median of 40 across 524 interventional studies indexed under Burns.
Browse Burns studies →Izmir Katip Celebi University is the lead sponsor of 191 studies on the registry; 40 are open to participants now.
Counted across the registry records on this site, refreshed daily.
Exclusion Criteria:
It will be accepted as the control group and 4 experimental burn wounds will be created on the back of the rats in the group and no treatment will be given.
Biological: burn wound dressing
4 experimental burn wounds will be created on the back of the rats in the group and dressing with 1% Silver Sulfadiazine will be done on the back of the rats every day for 21 days.
Biological: burn wound dressing
4 experimental burn wounds will be created on the back of the rats in the group and dressing with %0.2 Nitrafurozon will be done on the back of the rats every day for 21 days.
Biological: burn wound dressing
4 experimental burn wounds will be created on the back of the rats in the group and dressing with 10% Propolis will be done on the back of the rats every day for 21 days.
Biological: burn wound dressing
4 experimental burn wounds will be created on the back of the rats in the group and dressing with 15% Propolis will be done on the back of the rats every day for 21 days.
Biological: burn wound dressing
4 experimental burn wounds will be created on the back of the rats in the group and dressing with Propolis vehicle will be done on the back of the rats every day for 21 days.
Biological: burn wound dressing
1. st. group: It will be considered as a control group and rats will not be treated. 2. nd. group: Vehicle group, Propolis vehicle will be applied topically to rats for 21 days. 3. rd group: Silver Sulfadiazine group, 1% Silver Sulfadiazine will be applied topically to rats for 21 days. 4. th. group: Nitrafurozon group, 0.2% Nitrafurozon will be applied topically for 21 days. 5. th. group: 10% Propolis group, 10% Propolis will be applied topically for 21 days. 6. th. group: 15% Propolis group, 15% Propolis will be applied topically for 21 days.
wound size
photos will be taken every day to determine the wound size
Time frame: 21 days
bulla
examination of the skin at the light microscopic level. Bulla will be considered as present or absent.
Time frame: 3rd day of the research
bulla
examination of the skin at the light microscopic level. Bulla will be considered as present or absent.
Time frame: 7th day of the research
bulla
examination of the skin at the light microscopic level. Bulla will be considered as present or absent.
Time frame: 14th day of the research
bulla
examination of the skin at the light microscopic level. Erythemawill be considered as present or absent.
Time frame: 21st day day of the research
edema
examination of the skin at the light microscopic level. Edema will be considered as present or absent.
Time frame: 3rd day of the research
edema
examination of the skin at the light microscopic level. Edema will be considered as present or absent.
Time frame: 7th day of the research
edema
examination of the skin at the light microscopic level. Edema will be considered as present or absent.
Time frame: 14th day of the research
edema
examination of the skin at the light microscopic level. Edema will be considered as present or absent.
Time frame: 21st day of the research
inflammatory cell infiltration
examination of the skin at the light microscopic level. Inflammatory cell infiltration will be considered as present or absent.
Time frame: 3rd day of the research
inflammatory cell infiltration
examination of the skin at the light microscopic level. Inflammatory cell infiltration will be considered as present or absent.
Time frame: 7th day of the research
inflammatory cell infiltration
examination of the skin at the light microscopic level. Inflammatory cell infiltration will be considered as present or absent.
Time frame: 14th day of the research
inflammatory cell infiltration
examination of the skin at the light microscopic level. Inflammatory cell infiltration will be considered as present or absent.
Time frame: 21st day of the research
necrosis
examination of the skin at the light microscopic level. Necrosis will be considered as present or absent.
Time frame: 3rd day of the research
necrosis
examination of the skin at the light microscopic level. Necrosis will be considered as present or absent.
Time frame: 7th day of the research
necrosis
examination of the skin at the light microscopic level. Necrosis will be considered as present or absent.
Time frame: 14th day of the research
necrosis
examination of the skin at the light microscopic level. Necrosis will be considered as present or absent.
Time frame: 21st day of the research
ulceration
examination of the skin at the light microscopic level. Ulceration will be considered as present or absent.
Time frame: 3rd day of the research
ulceration
examination of the skin at the light microscopic level. Ulceration will be considered as present or absent.
Time frame: 7th day of the research
ulceration
examination of the skin at the light microscopic level. Ulceration will be considered as present or absent.
Time frame: 14th day of the research
ulceration
examination of the skin at the light microscopic level. Ulceration will be considered as present or absent.
Time frame: 21st day of the research
angiogenesis
examination of the skin at the light microscopic level. Angiogenesis will be considered as present or absent.
Time frame: 3rd day of the research
angiogenesis
examination of the skin at the light microscopic level. Angiogenesis will be considered as present or absent.
Time frame: 7th day of the research
angiogenesis
examination of the skin at the light microscopic level. Angiogenesis will be considered as present or absent.
Time frame: 14th day of the research
angiogenesis
examination of the skin at the light microscopic level. Angiogenesis will be considered as present or absent.
Time frame: 21st day of the research
fibroblast proliferation
examination of the skin at the light microscopic level. Fibroblast proliferation will be considered as present or absent.
Time frame: 3rd day of the research
fibroblast proliferation
examination of the skin at the light microscopic level. Fibroblast proliferation will be considered as present or absent.
Time frame: 7th day of the research
fibroblast proliferation
examination of the skin at the light microscopic level. Fibroblast proliferation will be considered as present or absent.
Time frame: 14th day of the research
fibroblast proliferation
examination of the skin at the light microscopic level. Fibroblast proliferation will be considered as present or absent.
Time frame: 21st day of the research
epithelization
examination of the skin at the light microscopic level. Epithelization will be considered as present or absent.
Time frame: 3rd day of the research
epithelization
examination of the skin at the light microscopic level. Epithelization will be considered as present or absent.
Time frame: 7th day of the research
epithelization
examination of the skin at the light microscopic level. Epithelization will be considered as present or absent.
Time frame: 14th day of the research
epithelization
examination of the skin at the light microscopic level. Epithelization will be considered as present or absent.
Time frame: 21st day of the research
collagenization
examination of the skin at the light microscopic level. Collagenization will be considered as present or absent.
Time frame: 3rd day of the research
collagenization
examination of the skin at the light microscopic level. Collagenization will be considered as present or absent.
Time frame: 7th day of the research
collagenization
examination of the skin at the light microscopic level. Collagenization will be considered as present or absent.
Time frame: 14th day of the research
collagenization
examination of the skin at the light microscopic level. Collagenization will be considered as present or absent.
Time frame: 21st day of the research
microorganism colonization
examination of the skin at the light microscopic level. Colonization will be considered as present or absent.
Time frame: 3rd day of the research
microorganism colonization
examination of the skin at the light microscopic level. Colonization will be considered as present or absent.
Time frame: 7th day of the research
microorganism colonization
examination of the skin at the light microscopic level. Colonization will be considered as present or absent.
Time frame: 14th day of the research
microorganism colonization
examination of the skin at the light microscopic level. Colonization will be considered as present or absent.
Time frame: 21st day of the research
Plan to share: No — the study's data is planned to be published and shared in a scientific paper
No publications or documents are linked to this record.
This study is completed, as verified in Aug 2022. You cannot join it, but the record below documents what was studied.
Get an email when the registry record changes — status, dates, results — or when someone posts here.
Sign in to followQuestions and observations about this study, from anyone following it. Not medical advice, and not a channel to the study team — their contact details are on the registry record.
Sign in to join the discussion. Reading takes no account; posting does. You choose a display name, and a pseudonym is the default.
Nothing here yet. If you are running this trial, taking part in it, or weighing whether to, this is the place to say so.
Izmir Katip Celebi University