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WithdrawnNCT03409341Updated Sep 24, 2020

Personalizing Immune Checkpoint Inhibitor Therapy

An observational study in Lung Cancer Stage IV, sponsored by Holy Cross Hospital, Maryland. Withdrawn at 1 site in United States. Open to participants aged 18 Years to 70 Years. Per ClinicalTrials.gov, last updated 2020-09-24.

Sponsored by Holy Cross Hospital, Maryland · Observational

Why this study was withdrawn
No enrollment
Study type
Observational
Model
Case-only
Time perspective
Prospective
Enrollment
0
Ages
18 Years to 70 Years
Sex
All
01

Study summary

This prospective, observational study will evaluate whether vitro testing of tumor tissue and white blood cells from patients with lung cancer who are being treated with immune checkpoint inhibitors and other standard of care approaches, predicts clinical response to these agents.

Read the detailed description

Overall Goal: The long-term goal of this study is to predict whether checkpoint inhibitors will be effective in individual patients with solid tumors.

Objectives: In this pilot study to be conducted in ten subjects with lung cancer who are to receive checkpoint inhibitors, we will use cellular and molecular techniques to characterize

  • tumor immunogenicity
  • anti-tumor T-cell response
  • effect of checkpoint inhibitors on antigen presentation and T-cell response, and
  • the tumor microenvironment

Planned enrollment:

  • Ten subjects with lung cancer

Methods for Informed Consent:

Investigators will approach subjects for informed consent

02

Conditions studied

  • Lung Cancer Stage IV
03

Who can participate

Ages eligible
18 Years to 70 Years
Sexes eligible
All
Sampling method
Non-probability sample

Study population

  • Ten subjects with lung cancer about to undergo diagnostic/excisional biopsy with the intent to receive therapy with immune checkpoint inhibitors

Inclusion criteria

  • Subjects with lung cancer about to undergo diagnostic/excisional biopsy with the intent to receive therapy with immune checkpoint inhibitors
  • Availability of fresh tumor tissue removed at excisional biopsy or diagnostic biopsy
  • Informed consent

Exclusion criteria

Exclusion Criteria:

  • Lack of informed consent
04

Study design

Observational model
Case-only
Time perspective
Prospective
Enrollment
0 participants (actual)
Patient registry
No
Biospecimen retention
Samples with dna

Interventions

  • OtherBlood and tumor tissue analysis, Clinical response.

    DNA sequencing and functional testing of peripheral blood mononuclear cells. DNA and RNA sequencing and immune cell analysis of tumor. Clinical response at 12 months.

05

What researchers measure

Primary outcomes

  1. Anti-tumor immune response

    Tumor antigen presentation and anti-tumor T-cell response

    Time frame: 12 months

Secondary outcomes

  1. Clinical response

    Clinical response is defined as 50% or greater decrease of the sum of the products of two-dimensional measurements of lesions on imaging within the first year.

    Time frame: 12 months for each subject

  2. Tumor microenvironment

    Tumor mutation burden from tumor genome landscape using DNA sequencing, tumor transcriptome with RNA sequencing, tumor infiltrating immune cells with flow cytometry

    Time frame: 24 months total study duration

06

Study locations

1 site
  • Holy Cross Hospital
    Silver Spring, Maryland 20910, United States
07

References and documents

Publications

  • Sindhi R, Ashokkumar C, Higgs BW. Cellular alloresponses for rejection-risk assessment after pediatric transplantation. Curr Opin Organ Transplant. 2011 Oct;16(5):515-21. doi: 10.1097/MOT.0b013e32834a94e3. PubMed 21844808 ↗
  • Ashokkumar C, Talukdar A, Sun Q, Higgs BW, Janosky J, Wilson P, Mazariegos G, Jaffe R, Demetris A, Dobberstein J, Soltys K, Bond G, Thomson AW, Zeevi A, Sindhi R. Allospecific CD154+ T cells associate with rejection risk after pediatric liver transplantation. Am J Transplant. 2009 Jan;9(1):179-91. doi: 10.1111/j.1600-6143.2008.02459.x. Epub 2008 Oct 31. PubMed 18976293 ↗
  • Ningappa M, Ashokkumar C, Higgs BW, Sun Q, Jaffe R, Mazariegos G, Li D, Weeks DE, Subramaniam S, Ferrell R, Hakonarson H, Sindhi R. Enhanced B Cell Alloantigen Presentation and Its Epigenetic Dysregulation in Liver Transplant Rejection. Am J Transplant. 2016 Feb;16(2):497-508. doi: 10.1111/ajt.13509. Epub 2015 Dec 11. PubMed 26663361 ↗
  • Ranganathan S, Ningappa M, Ashokkumar C, Higgs BW, Min J, Sun Q, Schmitt L, Subramaniam S, Hakonarson H, Sindhi R. Loss of EGFR-ASAP1 signaling in metastatic and unresectable hepatoblastoma. Sci Rep. 2016 Dec 2;6:38347. doi: 10.1038/srep38347. PubMed 27910913 ↗
  • Vinayak R, Cruz RJ Jr, Ranganathan S, Mohanka R, Mazariegos G, Soltys K, Bond G, Tadros S, Humar A, Marsh JW, Selby RR, Reyes J, Sun Q, Haberman K, Sindhi R. Pediatric liver transplantation for hepatocellular cancer and rare liver malignancies: US multicenter and single-center experience (1981-2015). Liver Transpl. 2017 Dec;23(12):1577-1588. doi: 10.1002/lt.24847. PubMed 28834194 ↗
  • Cruz RJ Jr, Ranganathan S, Mazariegos G, Soltys K, Nayyar N, Sun Q, Bond G, Shaw PH, Haberman K, Krishnamurti L, Marsh JW, Humar A, Sindhi R. Analysis of national and single-center incidence and survival after liver transplantation for hepatoblastoma: new trends and future opportunities. Surgery. 2013 Feb;153(2):150-9. doi: 10.1016/j.surg.2012.11.006. PubMed 23331862 ↗

Individual participant data

Plan to share: Yes — Data sharing: The data generated in this study will be available to The Holy Cross Health Institutional Review Board, Inc., Investigators named in this protocol, and Officials of the FDA and HHS. Published information following completion of the study will be provided to study patients.

Supporting information: Csr

08

Registry details

Key details

Study ID
NCT03409341
Lead sponsor
Holy Cross Hospital, Maryland
Responsible party
Sponsor
First posted
Jan 24, 2018
Start date
Feb 1, 2019
Primary completion
Jan 8, 2020
Completion
Sep 22, 2020
Last update
Sep 24, 2020

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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This study is withdrawn, as verified in Sep 2020. You cannot join it, but the record below documents what was studied.

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