An observational study in Vitamin D Deficiency, Infertility and Couples, sponsored by Tongji Hospital. Completed. Open to participants aged 20 Years to 42 Years. Per ClinicalTrials.gov, last updated 2017-10-10.
Sponsored by Tongji Hospital · Observational
Between March 2016 and March 2017, we performed a large retrospective, non-interventional, single-center cohort study and measured 25(OH)D3 plasma levels prior to ovarian stimulation in couples who underwent IVF/ICSI.And we analysed the correlation of vitamin D status and reproductive capacity .
Before conducting ovarian stimulation, all couples must conduct preoperative routine examination such as routine blood test, blood group examination and infectious disease examination. We collected 2ml remaining blood from the examination by use of sodium heparin, which then conducted 1,25(OH)2D3 assay by liquid chromatography-tandem mass spectrometry.
All patients underwent a standardized ovarian stimulation regimen, oocyte retrieval, and fertilization, followed by a planned fresh or frozen embryo transfer.Normal fertilization rate was defined as the presence of two pronuclei (2PN) at the time of fertilization assessment, 16-19 h after ICSI or conventional insemination. The percentage of top quality embryos was defined as the percentage of top quality embryos among the total number of 2PN embryos. Blastocyst forming rate was calculated as number of formed blastocysts devided by number of cultured blastocysts.
non-interventional, single-center cohort study and measured 25(OH) D plasma levels prior to ovarian stimulation in couples who underwent IVF/ICSI.
Exclusion Criteria:
The level of vitamin D is below 20ng/ml.
Diagnostic Test: vitamin D is below 20ng/ml
The level of vitamin D is between 20ng/ml and 30ng/ml.
The level of vitamin D is above 30ng/ml.
fertilization rate
Normal fertilization rate was defined as the presence of two pronuclei (2PN) at the time of fertilization assessment, 16-19 h after ICSI or conventional insemination. The percentage of top quality embryos was defined as the percentage of top quality embryos among the total number of 2PN embryos. Blastocyst forming rate was calculated as number of formed blastocysts devided by number of cultured blastocysts.
Time frame: the time of fertilization assessment, 16-19 hours after ICSI or conventional insemination
No study locations are listed for this record.
Plan to share: Undecided
This study is completed, as verified in Oct 2017. You cannot join it, but the record below documents what was studied.
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Tongji Hospital