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Active, not recruitingNCT03289780INSIGHTUpdated Sep 19, 2025

Clinical Effectiveness Assessment of VeriStrat® Testing and Validation of Immunotherapy Tests in NSCLC Subjects

An observational study in Non-Small Cell Lung Cancer, sponsored by Biodesix, Inc.. Active, not recruiting at 42 sites in United States. Open to participants aged 18 Years and older. Per ClinicalTrials.gov, last updated 2025-09-19.

Sponsored by Biodesix, Inc. · Observational

Study type
Observational
Model
Cohort
Time perspective
Prospective
Enrollment
5,006
Ages
18 Years and older
Sex
All
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Study summary

The purpose of this study is to collect information about how a doctor uses the results of the VeriStrat® blood test to guide treatment for non-small cell lung cancer (NSCLC) patients. Understanding how VeriStrat test results influence doctors' decisions and patients' outcomes may help doctors to better treat NSCLC in the future. This study will also look to establish whether new investigational tests can help better predict the effectiveness of certain medications for certain patients. These new investigational tests are only for research purposes at this time.

Read the detailed description

The primary purpose of this observational study is to assess the physician's clinical practice patterns while using VeriStrat testing in subjects with NSCLC whose tumors are epidermal growth factor receptor (EGFR) wild-type (negative) or have unknown EGFR mutational status. This study will also attempt to further validate that VeriStrat test results stratify subjects by clinical outcomes in the real world, uncontrolled clinical setting while exploring whether certain therapeutic approaches may yield opportunities for further study.

Predictive tests that aid physician therapeutic decision making are critical for optimizing subject outcomes while minimizing toxicity and associated treatment costs. This study will provide data for the validation of immunotherapy tests currently being developed. Immunotherapy mechanisms are dependent upon the interactions between the tumor, tumor microenvironment, and the patient immune system. As such, a successful predictive test will reflect the complex interplay between tumor and host. The multivariate tests from Biodesix have the advantage of being able to assess this complex biology.

The information gained from this research will not only guide the adoption of the VeriStrat test and inform medical decision making, including treatment choice, but will allow the validation of additional mass-spectrometry-based proteomic tests.

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Conditions studied

  • Non-Small Cell Lung Cancer
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Who can participate

Ages eligible
18 Years and older
Sexes eligible
All
Accepts healthy volunteers
No
Sampling method
Non-probability sample

Study population

Any Subject with Non-Small Cell Lung Cancer (NSCLC) at all stages with any histology.

Inclusion criteria

  1. Subject must be 18 years of age or older at time of signing informed consent form (ICF).
  2. A diagnosis of NSCLC.
  3. Subject is willing to provide serum samples for VeriStrat testing.
  4. EGFR mutation status wild-type (negative) or a tested unknown.
  5. For subjects with untested/unknown EGFR status only: The subject must be willing to provide blood samples for GeneStrat testing.
  6. Subject is willing to provide serum samples for research, understanding that no test results will be made available either to the subject or the treating physician.
  7. Subject is able to read and understand the ICF and agrees to comply with study procedures and requirements.

Exclusion criteria

Exclusion Criteria:

1. Subject's ability to understand the requirements of the protocol or to provide informed consent is impaired or subject is unwilling to comply with the protocol requirements.

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Study design

Observational model
Cohort
Time perspective
Prospective
Enrollment
5,006 participants (actual)
Patient registry
No
05

What researchers measure

Primary outcomes

  1. Physician Treatment Patterns Description

    Physician Treatment patterns Description: To describe physician treatment patterns pre and post-veriStrat testing. Describe the impact of the VeriStrat test results on treatment decisions, including but not limited to: percentage change in treatment decision, differences in chosen treatments between patients classified as VeriStrat good and those classified as VeriStrat Poor. Change in percentage of patients receiving systemic therapy or supportive therapies only.

    Time frame: 3 years

Secondary outcomes

  1. Immunotherapy Stratification by Overall Survival

    To determine whether immunotherapy test(s) stratify immunotherapy-treated subjects by overall survival.

    Time frame: 3 years

  2. Immunotherapy Stratification by Progression-Free Survival

    To determine whether immunotherapy test(s) stratify immunotherapy-treated subjects by progression-free survival.

    Time frame: 3 years

  3. VeriStrat Poor vs. Good Outcomes

    To compare progression free survival (PFS) and overall survival (OS) outcomes between those classified as VeriStrat-Poor and VeriStrat-Good.

    Time frame: 3 years

  4. Platinum-based therapy outcomes in VeriStrat Poor vs. Good subjects.

    To compare outcomes in subjects classified as VeriStrat-Poor and VeriStrat-Good and treated with platinum-based therapy.

    Time frame: 3 years

  5. Immunotherapy outcomes in VeriStrat Poor vs. Good subjects.

    To compare outcomes in subjects classified as VeriStrat-Poor and VeriStrat-Good and treated with immunotherapy.

    Time frame: 3 years

  6. Single agent chemotherapy outcomes in VeriStrat Poor vs. Good Subjects

    To compare outcomes in subjects classified as VeriStrat-Poor and VeriStrat-Good and treated with single agent chemotherapy.

    Time frame: 3 years

  7. VeriStrat label changes over time.

    To compare the longitudinal changes in VeriStrat classification over the course of the study

    Time frame: 3 years

Other outcomes

  1. Determination of Immunotherapy tests ability to stratify subjects based on treatment.

    To determine whether immunotherapy test(s) stratify subjects treated with chemotherapy or targeted therapies by outcome.

    Time frame: 3 years

  2. Correlation between the VeriStrat test and Immunotherapy tests.

    To observe the correlation between VeriStrat classification and immunotherapy test(s) classification at baseline and longitudinally.

    Time frame: 3 years

  3. Longitudinal changes in Immunotherapy tests.

    To describe the longitudinal changes in immunotherapy test classification over the course of the study.

    Time frame: 3 years

  4. Stratification of Immunotherapy Test(s)

    To determine whether immunotherapy test(s) stratify immunotherapy-treated subjects by other clinically meaningful factors or endpoints.

    Time frame: 3 years

  5. Changes in GeneStrat

    To observe changes in GeneStrat status across lines of therapy.

    Time frame: 3 years

06

Study locations

42 sites
  • Oncology Specialties, PC; Clearview Cancer Institute
    Huntsville, Alabama 35805, United States
  • Lewis & Faye Manderson Cancer Center at DCH Regional Medical Center
    Tuscaloosa, Alabama 35401, United States
  • Highlands Oncology Group
    Fayetteville, Arkansas 72703, United States
  • St. Bernards Cancer Center Hematology / Oncology
    Jonesboro, Arkansas 72401, United States
  • Valley Medical Oncology Consultants
    Pleasanton, California 94588, United States
  • Eastern Connecticut Hematology and Oncology Associates
    Norwich, Connecticut 06360, United States
  • Boca Raton Regional Hospital -Lynn Cancer Institute
    Boca Raton, Florida 33486, United States
  • Lake City Cancer Care, LLC
    Lake City, Florida 32024, United States
  • South Eastern Regional Medical Center
    Newnan, Georgia 30265, United States
  • Summit Cancer Care
    Savannah, Georgia 31405, United States
  • South Georgia Medical Center
    Valdosta, Georgia 31602, United States
  • Edward-Elmhurst Healthcare
    Naperville, Illinois 60540, United States
  • Illinois Cancer Care
    Peoria, Illinois 61615, United States
  • Franciscan St. Francis Health
    Indianapolis, Indiana 46237, United States
  • Franciscan Health Woodland Cancer Center
    Michigan City, Indiana 46360, United States
  • Ponchartrain Cancer Center
    Covington, Louisiana 70433, United States
  • Christus Health
    Shreveport, Louisiana 71105, United States
  • North Mississippi Medical Center - Hematology and Oncology Clinic
    Tupelo, Mississippi 38801, United States
  • Central Care Cancer Center
    Bolivar, Missouri 65613, United States
  • Phelps County Regional Medical Center
    Rolla, Missouri 65401, United States
  • Mercy Medical
    Springfield, Missouri 65804, United States
  • Nebraska Methodist Hospital
    Omaha, Nebraska 68114, United States
  • Essex Oncology
    Belleville, New Jersey 07109, United States
  • New York Oncology Group
    Albany, New York 12206, United States
  • Clinical Research Associates
    Lake Success, New York 11042, United States
  • NYU Laura & Isaac Perlmutter Cancer Center
    New York, New York 10016, United States
  • University of North Carolina
    Chapel Hill, North Carolina 27517, United States
  • Southeastern Medical Oncology
    Goldsboro, North Carolina 27534, United States
  • Leo W. Jenkins Cancer Center - East Carolina University
    Greenville, North Carolina 27834, United States
  • Hematology and Oncology Associates - Mercy Medical Center
    Canton, Ohio 44708, United States
  • Tri-County Hematology & Oncology Associates
    Massillon, Ohio 44646, United States
  • Oregon Oncology Specialists (QCCA)
    Salem, Oregon 97301, United States
  • Gettysburg Cancer Center
    Gettysburg, Pennsylvania 17325, United States
  • Bon Secours St. Francis Cancer Center
    Greenville, South Carolina 29607, United States
  • Arlington Cancer Center
    Arlington, Texas 76012, United States
  • JPS Health Network, JPS Center for Cancer Care
    Fort Worth, Texas 76104, United States
  • The Center for Cancer and Blood Disorders
    Fort Worth, Texas 76104, United States
  • Scott & White Memorial Hospital and Clinic
    Temple, Texas 76508, United States
  • Huntsman Cancer Institute
    Salt Lake City, Utah 84112, United States
  • Virginia Cancer Institute
    Richmond, Virginia 23230, United States
  • Peace Health
    Bellingham, Washington 982256, United States
  • Northwest Medical Specialties, PLLC
    Tacoma, Washington 98405, United States
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References and documents

Publications

  • Akerley WL, Arnaud AM, Reddy B, Page RD. Impact of a multivariate serum-based proteomic test on physician treatment recommendations for advanced non-small-cell lung cancer. Curr Med Res Opin. 2017 Jun;33(6):1091-1097. doi: 10.1080/03007995.2017.1301903. Epub 2017 Mar 16. PubMed 28277859 ↗
  • Grossi F, Rijavec E, Genova C, Barletta G, Biello F, Maggioni C, Burrafato G, Sini C, Dal Bello MG, Meyer K, Roder J, Roder H, Grigorieva J. Serum proteomic test in advanced non-squamous non-small cell lung cancer treated in first line with standard chemotherapy. Br J Cancer. 2017 Jan 3;116(1):36-43. doi: 10.1038/bjc.2016.387. Epub 2016 Nov 29. PubMed 27898657 ↗
  • Rich P, Mitchell RB, Schaefer E, Walker PR, Dubay JW, Boyd J, Oubre D, Page R, Khalil M, Sinha S, Boniol S, Halawani H, Santos ES, Brenner W, Orsini JM, Pauli E, Goldberg J, Veatch A, Haut M, Ghabach B, Bidyasar S, Quejada M, Khan W, Huang K, Traylor L, Akerley W. Real-world performance of blood-based proteomic profiling in first-line immunotherapy treatment in advanced stage non-small cell lung cancer. J Immunother Cancer. 2021 Oct;9(10):e002989. doi: 10.1136/jitc-2021-002989. PubMed 34706885 ↗

Related links

Study documents

  • Study protocol · Sep 18, 2017
  • Informed consent form · Sep 18, 2017

Documents are hosted by the registry — open the source record to download them.

Individual participant data

Plan to share: No

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Registry details

Key details

Study ID
NCT03289780
Lead sponsor
Biodesix, Inc.
Responsible party
Sponsor
First posted
Sep 21, 2017
Start date
Apr 4, 2016
Primary completion
Dec 2025 (estimated)
Completion
Dec 2025 (estimated)
Last update
Sep 19, 2025

Study contacts

Steve Springmeyer, MD
principal investigator · Biodesix, Inc.

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

Not currently enrolling

This study is active, not recruiting, as verified in Sep 2025. You cannot join it, but the record below documents what was studied.

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