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CompletedNCT03268902ELICITUpdated May 10, 2021

Early Life Interventions for Childhood Growth and Development In Tanzania

A Phase 2/3 interventional study of Azithromycin Oral Liquid Product and Nitazoxanide Oral Suspension in Malnutrition, Stunting and Cognitive Development, sponsored by Haydom Lutheran Hospital. Completed at 1 site in Tanzania. Open to participants aged 0 Days to 14 Days. Per ClinicalTrials.gov, last updated 2021-05-10.

Sponsored by Haydom Lutheran Hospital · Phase 2/3, Interventional, and Treatment

Phase
Phase 2/3
Study type
Interventional
Enrollment
1,188
Allocation
Randomized
Ages
0 Days to 14 Days
Sex
All
01

Study summary

This study aims to assess growth and cognitive effects of treatment with azithromycin and nitazoxanide and/or nicotinamide (vitamin B3) supplementation nicotinamide.

Read the detailed description

Children living in rural sub-Saharan Africa experience massive challenges to child thriving, with poor linear growth and delays in child development. In a cohort of 211 children living in the rural Haydom area of Tanzania (participating in the Interactions of Malnutrition \& Enteric Infections: Consequences for Child Health and Development "MAL-ED" Study), 70.6% had stunted growth at 18 months. This rate of moderate and severe stunting (length-for-age z-score [HAZ] \<-2 standard deviations) was the highest of the 8 study sites in MAL-ED.

This enormous deficit is likely associated with high rates of enteric infections with Campylobacter, E. coli pathotypes, Cryptosporidium, and Giardia, organisms susceptible to azithromycin and/or nitazoxanide. Infections such as these occur frequently in developing areas and are often associated with environmental enteropathy, including ongoing enteric inflammation and loss of enterocyte integrity, leading to possible bacterial translocation and poorer absorption of ingested nutrients. The consequences of these infections, enteric dysfunction and poor nutrient absorption frequently include growth stunting, learning delays, and an overall loss of human capital.

Emerging evidence suggests a potential role for the tryptophan-niacin pathway (including the end-product nicotinamide, an isoform of vitamin B3) in decreasing mucosal inflammation and affecting enteral microbiota. At the Tanzania site of MAL-ED, serum levels of tryptophan were related to subsequent linear growth, further suggesting importance of the tryptophan-niacin pathway. What is not clear is whether early childhood growth and development could be improved by targeting enteric infection and the tryptophan-niacin pathway by 1) delivering antibiotics against specific bacteria and/or 2) providing vitamin B3 as nicotinamide/niacinamide.

The main analysis will be intention-to-treat but a secondary analysis will be per protocol.

02

Conditions studied

  • Malnutrition
  • Stunting
  • Cognitive Development
  • Enteric Pathogens

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03

Who can participate

Ages eligible
0 Days to 14 Days
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  1. Maternal age ≥18
  2. Infant ≤ 14 days

Exclusion criteria

Exclusion Criteria:

  1. Maternal inability to adhere to protocol
  2. Multiple gestation
  3. Severe illness (significant birth defect, hospitalization, severe neonatal illness)
  4. Birth weight \<1500 g
  5. Lack of breastfeeding at enrollment (and lack of intention to continue breastfeeding at time of enrollment).
04

Study design

Phase
Phase 2 / Phase 3
Primary purpose
Treatment
Allocation
Randomized
Intervention model
Factorial assignment
Masking
Quadruple (Participant, Care provider, Investigator, Outcomes assessor)
Enrollment
1,188 participants (actual)

Study arms

  • Experimental
    Nicotinamide and Antimicrobials

    Nicotinamide Azithromycin Oral Liquid Product Nitazoxanide Oral Suspension

    Drug: Azithromycin Oral Liquid Product · Drug: Nitazoxanide Oral Suspension · Dietary Supplement: Nicotinamide

  • Experimental
    Antimicrobials only

    Placebo Azithromycin Oral Liquid Product Nitazoxanide Oral Suspension

    Drug: Azithromycin Oral Liquid Product · Drug: Nitazoxanide Oral Suspension · Drug: Placebos

  • Experimental
    Nicotinamide only

    Nicotinamide Placebo Placebo

    Dietary Supplement: Nicotinamide · Drug: Placebos

  • Placebo comparator
    No active treatment

    Placebo Placebo Placebo

    Drug: Placebos

Interventions

  • DrugAzithromycin Oral Liquid Product

    Azithromycin 20 mg/kg administered by study personnel at 6, 9, 12 and 15 months

    Also known as: Throza DPS

  • DrugNitazoxanide Oral Suspension

    Nitazoxanide 100 mg given twice daily for 3 days at 12 and 15 months

    Also known as: Alinia

  • Dietary supplementNicotinamide

    Mothers in the nicotinamide arm will be given nicotinamide 250 mg daily from delivery through 6 months post-partum in capsule form. Children in the nicotinamide arm will be given 100 mg/d in powder form between 6 and 18 months of age

    Also known as: Vitamin B3

  • DrugPlacebos

    Contain inert excipients only. Azithromycin placebo 20 mg/kg administered by study personnel at 6, 9, 12 and 15 months. Nitazoxanide placebo 100 mg given twice daily for 3 days at 12 and 15 months. Mothers in the nicotinamide placebo arm will be given placebo 250 mg daily from delivery through 6 months post-partum in capsule form. Children in the nicotinamide placebo arm will be given 100 mg/d of placebo in powder form between 6 and 18 months of age

05

What researchers measure

Primary outcomes

  1. Height-for-age z-score (HAZ) at 18 months

    Time frame: 18 months

Secondary outcomes

  1. Weight-for-age z-score (WAZ) at 18 months

    Time frame: 18 months

  2. Head circumference-for-age z-score (HCAZ) at 18 months

    Time frame: 18 months

  3. Stunting

    HAZ \<-2

    Time frame: 18 months

  4. All cause mortality

    Time frame: 0-18 months

  5. Hospitalization

    Time frame: 0-18 months

  6. Childhood illness

    Incidence of diarrhea, lower respiratory infection and febrile illness

    Time frame: 0-18 months

  7. Anemia

    Moderate to severe anemia by WHO definition for age and altitude

    Time frame: 12 and 18 months

  8. Enteropathogen burden

    Time frame: 6, 6.5, 12, 12.5, 18 months

  9. Microbiota composition

    Composition of intestinal microbiome

    Time frame: 6, 6.5, 12, 18 months

  10. Stool myeloperoxidase concentration

    Stool myeloperoxidase ELISA

    Time frame: 6, 12, 18 months

  11. C-reactive protein concentration in serum

    High-sensitivity CRP concentration

    Time frame: 12 and 18 months

  12. Insulin-like growth factor 1 concentration in serum

    Time frame: 12 and 18 months

  13. Collagen X concentration in serum

    Time frame: 12 and 18 months

  14. Tryptophan-kynurenine ratio

    Ratio of tryptophan concentration to kynurenine concentration in metabolomic testing

    Time frame: 12 and 18 months

  15. Niacin and nicotinamide metabolite concentration

    Concentration of downstream metabolites of niacin and nicotinamide as tested by metabolomic analysis

    Time frame: 6, 12, 18 months

  16. Small intestinal bacterial overgrowth

    Prevalence of SIBO as tested via exhaled hydrogen

    Time frame: 6, 12 and 18 months

  17. Malawi Developmental Assessment Tool score

    The MDAT is a measure of child cognitive development

    Time frame: 18 months

06

Study locations

1 site
  • Haydom Lutheran Hospital
    Haydom, Manyara, Tanzania
07

References and documents

Publications

  • DeBoer MD, Platts-Mills JA, Elwood SE, Scharf RJ, McDermid JM, Wanjuhi AW, Jatosh S, Katengu S, Parpia TC, Rogawski McQuade ET, Gratz J, Svensen E, Swann JR, Donowitz JR, Mdoe P, Kivuyo S, Houpt ER, Mduma E. Effect of scheduled antimicrobial and nicotinamide treatment on linear growth in children in rural Tanzania: A factorial randomized, double-blind, placebo-controlled trial. PLoS Med. 2021 Sep 28;18(9):e1003617. doi: 10.1371/journal.pmed.1003617. eCollection 2021 Sep. PubMed 34582462 ↗
  • DeBoer MD, Platts-Mills JA, Scharf RJ, McDermid JM, Wanjuhi AW, Gratz J, Svensen E, Swann JR, Donowitz JR, Jatosh S, Houpt ER, Mduma E. Early Life Interventions for Childhood Growth and Development in Tanzania (ELICIT): a protocol for a randomised factorial, double-blind, placebo-controlled trial of azithromycin, nitazoxanide and nicotinamide. BMJ Open. 2018 Jul 7;8(7):e021817. doi: 10.1136/bmjopen-2018-021817. PubMed 29982218 ↗

Study documents

  • Statistical analysis plan · Feb 5, 2020

Documents are hosted by the registry — open the source record to download them.

Individual participant data

Plan to share: No

08

Registry details

Key details

Study ID
NCT03268902
Lead sponsor
Haydom Lutheran Hospital
Collaborators
University of Virginia, Bill and Melinda Gates Foundation
Responsible party
Sponsor
First posted
Aug 31, 2017
Start date
Sep 5, 2017
Primary completion
Feb 28, 2020
Completion
Mar 26, 2020
Last update
May 10, 2021

Study contacts

Estomih Mduma
principal investigator · Haydom Lutheran Hospital

Oversight

Data monitoring committee
Yes
FDA-regulated drug
Yes
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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