CClinicalTrials.gg
CompletedNCT03258203Updated Aug 29, 2019

Gene-Environment Interaction and Precision Nutrition on Adiposity, Glycemic, Lipids Changes, and Bone Health

An interventional study of Preventing Overweight Using Novel Dietary Strategies in Overweight and Obesity, sponsored by Tulane University. Completed. Open to participants aged 30 Years to 70 Years. Per ClinicalTrials.gov, last updated 2019-08-29.

Sponsored by Tulane University · Not applicable, Interventional, and Prevention

Phase
Not applicable
Study type
Interventional
Enrollment
811
Allocation
Randomized
Ages
30 Years to 70 Years
Sex
All
01

Study summary

The purpose of this study is to test the interactions between the genes and diet interventions varying in macronutrient intake on glycemic, waist circumference, body weight, visceral fat and lipids changes. In the POUNDS LOST, all of the 811 participants DNA was extracted from the buffy coat fraction of centrifuged blood using the QIAmp blood kit (Qiagen, Chatsworth, CA). Single nucleotide polymorphisms were genotyped using the OpenArray™ SNP genotyping system (BioTrove, Woburn, MA). The study is only accepting participants in the Boston, Massachusetts or Baton Rouge, Louisiana area. For further enrollment information in Boston or Baton Rouge, see Eligibility Criteria or Design Narrative.

Besides, the investigators integrated novel pathway analyses in large cohorts and comprehensive genetic analyses on long-term weight loss and mechanisms in randomized diet intervention trials would provide very important evidence to unravel the etiology of obesity, and have significant public health and clinical implications. Establishing relationship between genetic variants and diets in determining weight change will help identify individuals at high risk for obesity especially when adherent to specific diet.

Read the detailed description

The epidemic of obesity has become a major public health problem. Obesity is a multifactorial abnormality that has a genetic basis but requires environmental influences to manifest. Common-form obesity is underpinned by both environmental and genetic factors. Recent genome-wide association studies have identified several genes convincingly related to obesity risk. Testing gene-environment interaction is a relatively new field. Evidence from association studies and intervention trials continues to mount, indicating that genetic components may modify lifestyle effects on the development of obesity. However, these findings are at most preliminary. The interplay between genetic and environmental components may facilitate the choice of more effective and specific measures for obesity prevention based on personalized genetic make-up.

The investigators aimed to examine whether the genetic variation affected glycemic, waist circumference, body weight, visceral fat and lipids changes in response to weight-loss dietary intervention varying in macronutrient intake (target percentages of energy derived from fat, protein, and carbohydrate in the 4 diets were as follows 20%, 15% and 65%; 20%, 25%, and 55%; 40%, 15%, and 45%; and 40%, 25%, and 35%).

The primary outcome is to test the interactions between the genes and diet interventions varying in macronutrient intake on body weight changes. The secondary outcome is to test the interactions between the genes and diet interventions varying in macronutrient intake on glycemic, waist circumference, visceral fat, and lipids changes. Understanding gene-diet interactions in relation to weight loss hold great promise for delivering more efficient prevention and treatment on obesity and related metabolic disorders.

In addition, besides gene-diet interactions, the investigators will also conduct other precision nutrition analysis. These analyses include interactions between pretreatment factors with diet intervention adiposity traits, initial changes induced by diet intervention with various outcomes measured in POUNDS Lost, including body weight, waist circumference, body composition, ectopic fat, and bone health.

02

Conditions studied

  • Overweight and Obesity
03

Who can participate

Ages eligible
30 Years to 70 Years
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  • Only accepting participants in the Boston, Massachusetts or Baton Rouge, Louisiana area
  • Between the ages of 30 - 70
  • Willing to modify eating pattern according to instructions
  • Overweight or obese and in good health with a BMI of 25-40
  • Committed to a long-term weight loss and maintenance program
  • Willing to attend multiple weight loss group sessions
  • Willing to increase activity level

Exclusion criteria

Exclusion Criteria:

  • Pregnant, planning to become pregnant, or are breastfeeding
  • Unwilling to participate in the schedule of group sessions and individual visits
  • Unstable or recent onset of heart disease or any other serious illness
  • Cannot change diet due to medical or other reasons
  • Planning to leave the area prior to the anticipated end of participation
  • Current participation in another clinical trial with an intervention that affects weight change
  • Have diabetes that is treated with insulin or hypoglycemic oral medicines
  • Diagnosis of psychiatric or emotional problems within 6 months of study
  • Currently have an eating disorder
  • Have hypothyroidism
  • Have an unstable weight
04

Study design

Phase
Not applicable
Primary purpose
Prevention
Allocation
Randomized
Intervention model
Factorial assignment
Masking
None (open label)
Enrollment
811 participants (actual)

Study arms

  • Experimental
    diet 1

    diet with moderate in fat (40% energy) and protein(15%)

    Other: Preventing Overweight Using Novel Dietary Strategies

  • Experimental
    diet 2

    diet with moderate in fat (40% energy) and protein(25%)

    Other: Preventing Overweight Using Novel Dietary Strategies

  • Experimental
    diet 3

    diet with low in fat (20% energy) and protein(15%)

    Other: Preventing Overweight Using Novel Dietary Strategies

  • Experimental
    diet 4

    diet with low in fat (20% energy) and protein(25%)

    Other: Preventing Overweight Using Novel Dietary Strategies

Interventions

  • OtherPreventing Overweight Using Novel Dietary Strategies

    The study tests the effectiveness for weight loss and weight maintenance of four diets differing in macronutrient composition: moderate in fat (40% energy) with two different protein levels (15% and 25%), and low in fat (20% energy), also with 15% and 25% protein levels.

05

What researchers measure

Primary outcomes

  1. Test the interactions between the genes and diet interventions varying in macronutrient intake on body weight changes(weight in kilograms) .

    Time frame: measured at Year 2

Secondary outcomes

  1. Test the interactions between the genes and diet interventions varying in macronutrient intake on glycemic changes (mg/dL for fasting glucose,uU/mL for fasting insulin).

    Time frame: measured at Year 2

  2. Test the interactions between the genes and diet interventions varying in macronutrient intake on waist circumference changes (cm).

    Time frame: measured at Year 2

  3. Test the interactions between the genes and diet interventions varying in macronutrient intake on visceral fat changes (%).

    Time frame: measured at Year 2

  4. Test the interactions between the genes and diet interventions varying in macronutrient intake on lipids changes (mg/dL for Total cholesterol, HDL cholesterol, LDL cholesterol and Triglycerides).

    Time frame: measured at Year 2

  5. Test the interactions between the genes and diet interventions varying in macronutrient intake on bone health (Bone mineral density at different skeletal sites).

    Bone mineral density was measured by dual-energy X-ray (DEXA)

    Time frame: measured at Year 2

Other outcomes

  1. Ectopic fat

    Computed tomography (CT) measured hepatic fat, abdominal fat distribution (includes visercal adipose tissue, subcutaneous adipose tissue, and total adipose tissue)

    Time frame: 2 year (measured at baseline, 6 months, and 2 years)

06

Study locations

No study locations are listed for this record.

07

References and documents

Publications

  • Zhou T, Sun D, Heianza Y, Li X, Champagne CM, LeBoff MS, Shang X, Pei X, Bray GA, Sacks FM, Qi L. Genetically determined vitamin D levels and change in bone density during a weight-loss diet intervention: the Preventing Overweight Using Novel Dietary Strategies (POUNDS Lost) Trial. Am J Clin Nutr. 2018 Nov 1;108(5):1129-1134. doi: 10.1093/ajcn/nqy197. PubMed 30475961 ↗
  • Han L, Ma W, Sun D, Heianza Y, Wang T, Zheng Y, Huang T, Duan D, Bray JGA, Champagne CM, Sacks FM, Qi L. Genetic variation of habitual coffee consumption and glycemic changes in response to weight-loss diet intervention: the Preventing Overweight Using Novel Dietary Strategies (POUNDS LOST) trial. Am J Clin Nutr. 2017 Nov;106(5):1321-1326. doi: 10.3945/ajcn.117.156232. Epub 2017 Sep 20. PubMed 28931532 ↗

Study documents

  • Protocol and statistical analysis plan · Apr 25, 2018

Documents are hosted by the registry — open the source record to download them.

Individual participant data

Plan to share: No

08

Registry details

Key details

Study ID
NCT03258203
Lead sponsor
Tulane University
Collaborators
Brigham and Women's Hospital
Responsible party
Lu Qi (Director of Tulane University Obesity Research Center, Tulane University) — Principal investigator
First posted
Aug 23, 2017
Start date
Sep 2003
Primary completion
Dec 2010
Completion
Dec 2010
Last update
Aug 29, 2019

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

Not currently enrolling

This study is completed, as verified in Aug 2019. You cannot join it, but the record below documents what was studied.

Follow this study

Get an email when the registry record changes — status, dates, results — or when someone posts here.

Sign in to follow

Discussion

Questions and observations about this study, from anyone following it. Not medical advice, and not a channel to the study team — their contact details are on the registry record.

Sign in to join the discussion. Reading takes no account; posting does. You choose a display name, and a pseudonym is the default.

Nothing here yet. If you are running this trial, taking part in it, or weighing whether to, this is the place to say so.

Start the discussion