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CompletedNCT03257085CALIBERUpdated Mar 8, 2018

CALIBER Phase 1: A Pilot Study in Normal-weight and Overweight Adults.

An interventional study of Low-carbohydrate, high-fat and High-carbohydrate, moderate-fat in Cardiometabolic Risk, sponsored by Liverpool John Moores University. Completed at 1 site in United Kingdom. Open to participants aged 19 Years to 64 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2018-03-08.

Sponsored by Liverpool John Moores University · Not applicable, Interventional, and Prevention

Phase
Not applicable
Study type
Interventional
Enrollment
15
Allocation
Randomized
Ages
19 Years to 64 Years
Sex
All
01

Study summary

Pilot study to compare the impact of following a low-carbohydrate, high-fat diet versus following a high-carbohydrates, moderate-fat diet (UK dietary guidelines) on cardiometabolic risk markers and associated behaviours in a normal-weight and overweight adult population.

Read the detailed description

Cardiometabolic diseases (CMD), such as type 2 diabetes and cardiovascular disease (CVD), are globally amongst the highest contributors to morbidity and mortality with high (cost) implications to the overall economy and health care systems. A number of risk markers have been associated with CMDs, including blood serum markers, low levels of lean mass and high levels of body fat, including increased waist circumference. Dietary factors and nutritional status have long been linked with specific markers of cardiometabolic (CM) risk. The quantity and quality of dietary carbohydrates has been associated with increased serum triglycerides levels, increased body fat mass, increased waist circumference and visceral fat around the organs in particular. They also seem to increase food cravings. Whilst official dietary guidelines in the UK and elsewhere still recommend a high carbohydrate and low fat diet as standard, these recommendations have increasingly been challenged. Evidence has been mounting that very-low carbohydrate (ketogenic) and low carbohydrate diets can ameliorate CM risk factors, especially when a personalised rather than a one-size-fits-all approach is being taken. Response to carbohydrate load and adherence to dietary interventions can vary widely dependent on individual substrate and energy metabolism and insulin-resistant status.

The majority of dietary interventions with ketogenic and low-carbohydrate diets has focused on weight loss as the primary outcome in overweight and obese individuals. However, in recent years evidence has been mounting that the location and quality of adipose tissue (AT) play a more important role in manifestation of CM risk than quantity of AT alone. Detrimental health behaviours, such as low-quality diet and low levels of physical activity seem to be important contributors to this.

Further studies can provide vital insights into the links between diet, location-specific adipose tissue, CM risk factors and health-related behaviours.

Therefore this 8-weeks randomised pilot study will investigate the impact of either following a low-carbohydrate, high-fat diet versus following a high-carbohydrates, moderate-fat diet (UK dietary guidelines) on cardiometabolic risk markers and associated behaviours in a normal-weight and overweight adult population aged 19 - 64 at potential risk of CMD.

02

Conditions studied

  • Cardiometabolic Risk

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03

Who can participate

Ages eligible
19 Years to 64 Years
Sexes eligible
All
Accepts healthy volunteers
Yes

Eligibility criteria

Inclusion Criteria cohort 1:

  1. BMI 18.5 - 29.9kg/m2
  2. Aged 19 - 64
  3. White-Caucasian
  4. Score of ≥4 from a combination of risk markers, including

    • Fasting glucose concentration >5.5mmol/L = 3 points
    • Waist >102cm or 40 inches = 2 points (males)
    • Waist >94 cm or 37 inches = 1 point (males)
    • Waist >88cm or 34inches = 2 points (females)
    • Waist >80 cm or 31 inches = 1 point (females)
    • Systolic blood pressure>130mmHg = 1 point
    • Diastolic BP >85mmHg = 1 point
    • HDL cholesterol concentration \<1.0mmol/L = 2 points
    • Serum triglyceride concentration >1.3mmol/L = 1 point.

Exclusion Criteria cohort 1:

  1. Smoker
  2. Vegetarian or vegan
  3. Suffering from food allergies or intolerances
  4. Drinking alcohol above recommended UK government guidelines
  5. Previous diagnosis of CM disease
  6. Taking lipid-lowering medication
  7. Taking blood pressure-lowering medication
  8. Taking blood glucose-lowering medication
  9. Taking dietary supplements
  10. Suffering from an eating disorder
  11. Current or previous renal impairment

Inclusion Criteria cohort 2:

  1. BMI 18.5 - 29.9kg/m2
  2. Aged 19 - 64
  3. White-Caucasian

Exclusion Criteria cohort 2:

  1. Smoker
  2. Vegetarian or vegan
  3. Suffering from food allergies or intolerances
  4. Drinking alcohol above recommended UK government guidelines
  5. Previous diagnosis of CM disease
  6. Taking lipid-lowering medication
  7. Taking blood pressure-lowering medication
  8. Taking blood glucose-lowering medication
  9. Taking dietary supplements
  10. Suffering from an eating disorder
  11. Current or previous renal impairment
04

Study design

Phase
Not applicable
Primary purpose
Prevention
Allocation
Randomized
Intervention model
Parallel assignment
Masking
None (open label)
Enrollment
15 participants (actual)

Study arms

  • Experimental
    Low-carbohydrate, high-fat

    Participants adhering to low-carbohydrate, high-fat diet for 8 weeks.

    Other: Low-carbohydrate, high-fat

  • Experimental
    High-carbohydrate, moderate fat

    Participants following high-carbohydrate, moderate-fat diet for 8 weeks.

    Other: High-carbohydrate, moderate-fat

Interventions

  • OtherLow-carbohydrate, high-fat

    Participants following low-carbohydrate, high-fat diet for 8 weeks.

    Also known as: CALIBER LCHF

  • OtherHigh-carbohydrate, moderate-fat

    Participants following high-carbohydrate, moderate-fat diet (UK dietary guidelines) for 8 weeks.

    Also known as: CALIBER HCMF

05

What researchers measure

Primary outcomes

  1. Serum lipid profile

    Total cholesterol, HDL-C, LDL-C, non-HDL cholesterol, small-dense LDL-C and triglycerides measured in mmol/L

    Time frame: 8 weeks

  2. Blood glucose

    Measured in mmol/L

    Time frame: 8 weeks

  3. Systolic and diastolic blood pressure

    Measured in mmHg

    Time frame: 8 weeks

  4. Inflammatory markers, such as CRP

    Measured in mg/L

    Time frame: 8 weeks

  5. Adiponectin

    Measured in μg/mL

    Time frame: 8 weeks

  6. Fibroblast growth factor 21 (FGF21).

    Measured in pg/mL

    Time frame: 8 weeks

  7. Tumor necrosis factor alpha and Interleukin 6

    Measured in μg/mL

    Time frame: 8 weeks

  8. Body composition - Bioelectrical impedance

    Lean mass, fat mass and adipose tissue location and distribution

    Time frame: 8 weeks

  9. Body composition - Anthropometrics

    Waist, hip, neck, thigh and calf circumference measured in cm

    Time frame: 8 weeks

Secondary outcomes

  1. Clinical traditional and emerging markers of dietary intake

    Fibroblast growth factor 21 and serum metabolites

    Time frame: 8 weeks

  2. Food cravings

    Measured via self-reported questionnaire

    Time frame: 8 weeks

  3. Satiety

    Assessed via serum leptin levels (ng/mL)

    Time frame: 8 weeks

  4. Cognition

    Assessed via self-reported questionnaire

    Time frame: 8 weeks

  5. Impact on physical activity patterns

    Assessed via accelerometry

    Time frame: 8 weeks

  6. Adherence to assigned diet

    Measured via 4-day food diaries

    Time frame: 8 weeks

  7. Adherence to dietary guidelines

    Measured via diet quality score assessment

    Time frame: 8 weeks

  8. Adherence to low-carbohydrate diet

    Measured via blood ketones (mmol/L)

    Time frame: 8 weeks

  9. Adherence to fibre recommendations

    Measured via structured questionnaires

    Time frame: 8 weeks

  10. Adherence to taking dietary supplement for low-carbohydrate, high-fat group

    Measured via counting of number of multi-vitamin and mineral supplements consumed

    Time frame: 8 weeks

  11. Experience with either low-carbohydrate, high-fat or high-carbohydrate, moderate-fat diet

    Assessed via semi-structured interview

    Time frame: 8 weeks

06

Study locations

1 site
  • Liverpool John Moores University
    Liverpool, Merseyside L17 6BD, United Kingdom
07

References and documents

Related links

Individual participant data

Plan to share: Undecided

08

Registry details

Key details

Study ID
NCT03257085
Lead sponsor
Liverpool John Moores University
Collaborators
Liverpool Hope University
Responsible party
Tanja Harrison (PhD Researcher and Registered Associate Nutritionist, Liverpool John Moores University) — Principal investigator
First posted
Aug 22, 2017
Start date
Mar 9, 2017
Primary completion
Dec 8, 2017
Completion
Dec 8, 2017
Last update
Mar 8, 2018

Study contacts

Ian G Davies, PhD
study chair · Liverpool John Moores University

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
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