CClinicalTrials.gg
CompletedNCT02935686IPCAVD-012Updated May 25, 2025Results posted

A Safety, Tolerability and Immunogenicity Study of 2 Different Regimens of Tetravalent Ad26.Mos4.HIV Prime Followed by Boost With Tetravalent Ad26.Mos4.HIV Along With Either Clade C gp140 Plus Adjuvant OR With a Combination of Mosaic and Clade C gp140 Plus Adjuvant in Healthy HIV Uninfected Adults

A Phase 1/2 interventional study of Ad26.Mos4.HIV and Clade C gp140 plus adjuvant in Healthy, sponsored by Janssen Vaccines & Prevention B.V.. Completed at 13 sites in 3 countries. Open to participants aged 18 Years and older, including healthy volunteers. Per ClinicalTrials.gov, last updated 2025-05-25.

Sponsored by Janssen Vaccines & Prevention B.V. · Phase 1/2, Interventional, and Prevention

Phase
Phase 1/2
Study type
Interventional
Enrollment
155
Allocation
Randomized
Ages
18 Years and older
Sex
All
01

Study summary

The primary purpose of this study is to assess safety/tolerability of the different vaccine regimens and of a late boost vaccination; and to assess envelope (Env)-binding antibody (Ab) responses of the 2 different vaccine regimens.

Read the detailed description

This is a randomized (study medication assigned by chance), double-blind (neither physician nor participant knows the treatment received), placebo-controlled (placebo is an inactive substance that is compared with a drug to test whether the drug has a real effect in a clinical trial), parallel-group (each treatment group will be treated at the same time), multicenter (more than one clinical site) study in healthy human immunodeficiency virus (HIV)-uninfected adults. The main study will be conducted in 3 phases: a 6-week screening period; a 48-week vaccination period; and a follow-up period to the final main study visit at Week 72. A Long-term Extension (LTE) phase (approximately 3 years after Week 72) will be performed for participants randomized to Group 1 or Group 2, who receive all 4 vaccinations and are negative for HIV infection at Week 72. The approximate duration of the study will be approximately 78 weeks for participants not participating in the LTE phase and approximately 222 weeks for participants participating in the LTE phase but not receiving a late boost vaccination and approximately 246 (12-month follow-up) or 294 (24-month follow-up) weeks for participants receiving a late boost vaccination. Participants safety will be monitored throughout the study.

02

Conditions studied

  • Healthy
03

Who can participate

Ages eligible
18 Years and older
Sexes eligible
All
Accepts healthy volunteers
Yes

Inclusion criteria

  • Participant must be healthy on the basis of medical history, physical examination, and vital signs measurement performed at screening
  • Participants are negative for human immunodeficiency virus (HIV) infection at screening
  • Participants are amenable to HIV-risk reduction counseling and committed to maintaining behavior consistent with low risk of HIV exposure through the last required protocol clinic visit
  • All female participants of childbearing potential must have a negative serum (beta-human chorionic gonadotropin [beta-hCG]) at the screening visit, and a negative urine pregnancy test pre-dose on Day 1
  • Participants are willing/able to adhere to the prohibitions and restrictions specified in the protocol and study procedures
  • Participant must be enrolled in the LTE phase to receive the late boost vaccination

Exclusion criteria

Exclusion Criteria:

  • Has chronic hepatitis B (measured by hepatitis B surface antigen test) or active hepatitis C (measured by hepatitis C virus [HCV] Ab test; if positive, HCV ribonucleic acid [RNA] polymerase chain reaction (PCR) test will be used to confirm active versus past HCV infection), active syphilis infection, chlamydia, gonorrhea, or trichomonas
  • In the 12 months prior to randomization, participant has a history of newly acquired herpes simplex virus type 2 (HSV-2), syphilis, gonorrhea, non-gonococcal urethritis, chlamydia, pelvic inflammatory disease, trichomonas, mucopurulent cervicitis, epididymitis, proctitis, lymphogranulomavenereum, chancroid, or hepatitis B
  • Participant has had major surgery (eg, requiring general anesthesia) within the 4 weeks before screening, or will not have fully recovered from surgery, or has surgery planned through the course of the study
  • Participant has had a thyroidectomy or active thyroid disease requiring medication during the last 12 months (not excluded: a stable thyroid supplementation)
  • Current or past drug/alcohol use that investigator assesses poses any more than a remotely increased risk of the ability of the participant to comply with the protocol requirements
  • Has been in receipt of any licensed vaccine within 14 days prior to the first dose of study vaccine or placebo, plans to receive within 14 days after the first study vaccination, or plans to receive within 14 days before or after the second, third or fourth vaccination
  • Is a recipient of a prophylactic or therapeutic HIV vaccine candidate at any time, or a recipient of other experimental vaccine(s) within the last 12 months prior to the Day 1 visit (Vaccination 1). For participants who received an experimental vaccine (except HIV vaccine) more than 12 months prior to the Day 1 visit (Vaccination 1), documentation of the identity of the experimental vaccine must be provided to the sponsor, who will determine eligibility on a case-by-case basis
04

Study design

Phase
Phase 1 / Phase 2
Primary purpose
Prevention
Allocation
Randomized
Intervention model
Parallel assignment
Masking
Double (Participant, Investigator)
Enrollment
155 participants (actual)

Study arms

  • Experimental
    Group 1: Ad26.Mos4.HIV + Clade C gp140

    Participants will receive Ad26.Mos4.HIV vaccine at Week 0 and 12, followed by Ad26.Mos4.HIV vaccine + Clade C glycoprotein 140 vaccine containing 250 microgram (mcg) of total protein mixed with adjuvant (aluminium phosphate) at Week 24 and 48. Participants who receive all 4 vaccinations and are negative for HIV infection at Week 72 can consent to be included in a long-term extension (LTE) phase (approximately 3 years after Week 72).

    Biological: Ad26.Mos4.HIV · Biological: Clade C gp140 plus adjuvant

  • Experimental
    Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140

    Participants will receive Ad26.Mos4.HIV vaccine at Week 0 and 12; followed by Ad26.Mos4.HIV vaccine + combination of 125 mcg Mosaic gp140 and 125 mcg Clade C gp140 mixed with adjuvant (aluminum phosphate) at Week 24 and 48. Participants who receive all 4 vaccinations and are negative for HIV infection at Week 72 can consent to be included in a long-term extension (LTE) phase (approximately 3 years after Week 72).

    Biological: Ad26.Mos4.HIV · Biological: Clade C gp140/Mosaic gp140 plus adjuvant

  • Placebo comparator
    Group 3: Placebo

    Participants will receive a single placebo injection at Weeks 0 and 12, followed by two placebo injections at Weeks 24 and 48.

    Other: Placebo

  • Experimental
    Group 1b: Ad26.Mos4.HIV + gp140 HIV Bivalent Vaccine

    Participants enrolled in the LTE phase will receive late boost vaccination Ad26.Mos4.HIV and bivalent gp140 within 4 weeks prior to Week 192 until 4 months after Week 192 (that is, approximately 3 years after the 4th vaccination of the primary vaccination series).

    Biological: Ad26.Mos4.HIV · Biological: gp140 HIV Bivalent Vaccine

  • Placebo comparator
    Group 2b: Placebo

    Participants will receive placebo injection at Week 192 -4 weeks/+4 months, that is, approximately 3 years after the 4th vaccination of the primary vaccination series.

    Other: Placebo

Interventions

  • BiologicalAd26.Mos4.HIV

    Ad26.Mos4.HIV at a dose of 5\*10\^10 viral particles (vp), administered intramuscularly.

  • BiologicalClade C gp140 plus adjuvant

    Clade C gp140 vaccine containing 250 mcg of total protein, mixed with aluminum phosphate adjuvant, per 0.5 milliliter (mL) injection administered intramuscularly.

  • BiologicalClade C gp140/Mosaic gp140 plus adjuvant

    Clade C gp140 and Mosaic gp140 (each 125 mcg of total protein) mixed with aluminum phosphate adjuvant, per 0.5 milliliter (mL) injection, administered intramuscularly.

  • OtherPlacebo

    Placebo Containing 0.9 percent normal saline, administered intramuscularly.

  • Biologicalgp140 HIV Bivalent Vaccine

    gp140 HIV Bivalent Vaccine is adjuvanted protein co-formulation with a dosage strength of 80 mcg Clade C protein, 75 mcg Mosaic protein and 425 mcg aluminum (as aluminum phosphate adjuvant).

05

What researchers measure

Primary outcomes

  1. Main Study: Number of Participants With Solicited Local and Systemic Adverse Events (AEs) for 7 Days Post-vaccination 1

    Number of participants with solicited local and systemic AEs for 7 days post-vaccination 1 were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment. Solicited AEs were precisely local and systemic events for which the participant is specifically questioned and symptoms of which were noted by participant in their diary. Solicited local AEs included injection site pain/tenderness, erythema, and swelling/induration at the study vaccine injection site. Solicited systemic AEs included fever (temperature measurement), fatigue, headache, nausea, myalgia, and chills.

    Time frame: Up to 7 days post-vaccination 1 on Day 1 (up to Day 8)

  2. Main Study: Number of Participants With Solicited Local and Systemic Adverse Events (AEs) for 7 Days Post-vaccination 2

    Number of participants with solicited local and systemic AEs for 7 days post-vaccination 2 were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment. Solicited AEs were precisely local and systemic events for which the participant is specifically questioned and symptoms of which were noted by participant in their diary. Solicited local AEs included injection site pain/tenderness, erythema, and swelling/induration at the study vaccine injection site. Solicited systemic AEs included fever (temperature measurement), fatigue, headache, nausea, myalgia, and chills.

    Time frame: Up to 7 days post vaccination 2 (up to any day from Day 78 to Day 113) (vaccination 2 ranged from Day 78 to 106)

  3. Main Study: Number of Participants With Solicited Local and Systemic Adverse Events (AEs) for 7 Days Post-vaccination 3

    Number of participants with solicited local and systemic AEs for 7 days post-vaccination 3 were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment. Solicited AEs were precisely local and systemic events for which the participant is specifically questioned and symptoms of which were noted by participant in their diary. Solicited local AEs included injection site pain/tenderness, erythema, and swelling/induration at the study vaccine injection site. Solicited systemic AEs included fever (temperature measurement), fatigue, headache, nausea, myalgia, and chills.

    Time frame: Up to 7 days post vaccination 3 (up to any day from Day 162 to Day 197) (vaccination 3 ranged from Day 162 to 190)

  4. Main Study: Number of Participants With Solicited Local and Systemic Adverse Events (AEs) for 7 Days Post-vaccination 4

    Number of participants with solicited local and systemic AEs for 7 days post-vaccination 4 were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment. Solicited AEs were precisely local and systemic events for which the participant is specifically questioned and symptoms of which were noted by participant in their diary. Solicited local AEs included injection site pain/tenderness, erythema, and swelling/induration at the study vaccine injection site. Solicited systemic AEs included fever (temperature measurement), fatigue, headache, nausea, myalgia, and chills.

    Time frame: Up to 7 days post vaccination 4 (up to any day from Day 330 to Day 365) (vaccination 4 ranged from Day 330 to 358)

  5. Main Study: Number of Participants With Unsolicited Adverse Events (AEs) for 28 Days Post-vaccination 1

    Number of participants with unsolicited AEs for 28 days post-vaccination 1 were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment. Unsolicited AEs were all AEs for which the participant is not specifically questioned in the participant diary.

    Time frame: Up to 28 days post-vaccination 1 on Day 1 (Up to Day 29)

  6. Main Study: Number of Participants With Unsolicited Adverse Events (AEs) for 28 Days Post-vaccination 2

    Number of participants with unsolicited AEs for 28 days post-vaccination 2 were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment. Unsolicited AEs were all AEs for which the participant is not specifically questioned in the participant diary.

    Time frame: Up to 28 days post vaccination 2 (up to any day from Day 78 to Day 134) (vaccination 2 ranged from Day 78 to 106)

  7. Main Study: Number of Participants With Unsolicited Adverse Events (AEs) for 28 Days Post-vaccination 3

    Number of participants with unsolicited AEs for 28 days post-vaccination 3 were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment. Unsolicited AEs were all AEs for which the participant is not specifically questioned in the participant diary.

    Time frame: Up to 28 days post vaccination 3 (up to any day from Day 162 to Day 218) (vaccination 3 ranged from Day 162 to 190)

  8. Main Study: Number of Participants With Unsolicited Adverse Events (AEs) for 28 Days Post-vaccination 4

    Number of participants with unsolicited AEs for 28 days post-vaccination 4 were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment. Unsolicited AEs were all AEs for which the participant is not specifically questioned in the participant diary.

    Time frame: Up to 28 days post vaccination 4 (up to any day from Day 330 to Day 358) (vaccination 4 ranged from Day 330 to 358)

  9. Main Study: Number of Participants Who Discontinued Study Vaccination Due to AEs

    Number of participants who discontinued study vaccination due to AEs were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment.

    Time frame: From Baseline (Day 1) up to Week 72

  10. Main Study: Number of Participants With Serious Adverse Events (SAEs)

    Number of participants with SAEs were reported. An AE was any untoward medical occurrence in a clinical study administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the study vaccine. An SAE is an AE resulting in any of the following outcomes or deemed significant for any other reason: death; initial or prolonged inpatient hospitalization; life-threatening experience (immediate risk of dying); persistent or significant disability/incapacity; congenital anomaly/birth defect; suspected transmission of any infectious agent via a medicinal product or medically important.

    Time frame: From Baseline (Day 1) up to Week 72

  11. Main Study and LTE Study: Number of Participants With Adverse Events of Special Interest (AESIs)

    Number of participants with adverse events of special interest (AESIs) were reported. As planned, confirmed HIV infection was the only event assessed as an AESI. AESIs (including potential AESIs) are significant AEs that are judged to be of special interest because of clinical importance, known or suspected class effects, or based on nonclinical signals. AESIs (including potential AESIs) must be reported to the sponsor within 24 hours of awareness irrespective of seriousness (that is, serious and nonserious AEs) or causality.

    Time frame: From Baseline (Day 1) up to Week 216

  12. Late-boost (LB) Vaccination Phase: Number of Participants Who Discontinued Study Due to AEs

    Number of participants who discontinued study due to AEs were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment.

    Time frame: From Week 188 up to end of study (Week 288)

  13. Late-boost (LB) Vaccination Phase: Number of Participants With Solicited Local and Systemic Adverse Events (AEs) for 7 Days Post Late Boost Vaccination

    Number of participants with solicited local and systemic AEs for 7 days post late boost vaccination were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment. Solicited AEs were precisely local and systemic events for which the participant is specifically questioned and symptoms of which were noted by participant in their diary. Solicited local AEs included injection site pain/tenderness, erythema, and swelling/induration at the study vaccine injection site. Solicited systemic AEs included fever (temperature measurement), fatigue, headache, nausea, myalgia, and chills.

    Time frame: Up to 7 days post late boost vaccination (up to any day from Day 1317 to Day 1464) (late boost vaccination ranged from Day 1317 to 1457)

  14. Late-boost (LB) Vaccination Phase: Number of Participants With Unsolicited Adverse Events (AEs) for 28 Days Post Late Boost Vaccination

    Number of participants with unsolicited AEs for 28 post late boost vaccination were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment. Unsolicited AEs were all AEs for which the participant is not specifically questioned in the participant diary.

    Time frame: Up to 28 days post late boost vaccination (up to any day from Day 1317 to Day 1485) (late boost vaccination ranged from Day 1317 to 1457)

  15. Late-boost (LB) Vaccination Phase: Number of Participants With Serious Adverse Events (SAEs)

    Number of participants with SAEs were reported. An AE was any untoward medical occurrence in a clinical study administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the study vaccine. An SAE is an AE resulting in any of the following outcomes or deemed significant for any other reason: death; initial or prolonged inpatient hospitalization; life-threatening experience (immediate risk of dying); persistent or significant disability/incapacity; congenital anomaly/birth defect; suspected transmission of any infectious agent via a medicinal product or medically important.

    Time frame: From Week 188 up to end of study (Week 288)

  16. Late-boost (LB) Vaccination Phase: Number of Participants With AESIs of HIV Infection Up to End of Study

    Number of participants with AESIs up to the end of the study were reported. An AE was any untoward medical occurrence in a clinical study administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the study vaccine. AESIs (including potential AESIs) are significant AEs that are judged to be of special interest because of clinical importance, known or suspected class effects, or based on nonclinical signals. AESIs (including potential AESIs) must be reported to the sponsor within 24 hours of awareness irrespective of seriousness (i.e, serious and nonserious AEs) or causality. Confirmed HIV infection was considered an AESI.

    Time frame: From Week 188 up to end of study (Week 288)

  17. Late-boost (LB) Vaccination Phase: Number of Participants With AESIs of Thrombosis With Thrombocytopenia Syndrome (TTS)

    Number of participants with AESIs of TTS were reported. An AE was any untoward medical occurrence in a clinical study administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the study vaccine. AESIs are significant AEs that are judged to be of special interest because of clinical importance, known or suspected class effects, or based on nonclinical signals. AESIs must be reported to the sponsor within 24 hours of awareness irrespective of seriousness (i.e, serious and nonserious AEs) or causality. Thrombotic events and/or thrombocytopenia were considered as AESIs.

    Time frame: Up to 6 months post late boost vaccination (up to any day from Day 1317 to Day 1639) (late boost vaccination ranged from Day 1317 to 1457)

  18. Main Study: Geometric Mean of Envelope (Env) Clade A (92UG037.1), B (1990a), C (Con C), (C97ZA.012), Mos 1 Specific Binding Antibodies (Abs) Responses As Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 28

    Geometric mean of Env Clade A (92UG037.1), B (1990a), C (Con C), (C97ZA.012), Mos 1 specific binding Abs responses were assessed using ELISA.

    Time frame: Week 28

  19. Main Study: Geometric Mean of Envelope (Env) Clade A (92UG037.1), B (1990a), C (Con C), (C97ZA.012), Mos 1 Specific Binding Antibodies (Abs) Responses As Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 52

    Geometric mean of Env Clade A (92UG037.1), B (1990a), C (Con C), (C97ZA.012), Mos 1 specific binding Abs responses were assessed using ELISA.

    Time frame: Week 52

  20. Main Study: Geometric Mean of Envelope (Env) Clade A (92UG037.1) B (1990a), C (Con C), (C97ZA.012), Mos 1 Specific Binding Antibodies (Abs) Responses As Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 72

    Geometric mean of Env Clade A (92UG037.1) B (1990a), C (Con C), (C97ZA.012), Mos 1 specific binding Abs responses were assessed using ELISA.

    Time frame: Week 72

  21. Main Study: Percentage of Responders for Envelope (Env)-Specific Binding Antibody Titers at Week 28

    Percentage of responders for envelop (Env) Clade B (SC42261), Clade C (CH505TF), Clade A (9004S), Clade B (RHPA), Clade B (WITO), Clade C (1086C), Clade C (BF1266), CladeAE (conAE), Clade M(Con S)-specific binding antibody titers assessed using enzyme-linked immunosorbent assay (ELISA) were reported. The response was defined as post-baseline value greater than (\>) lower limit of quantification (LLOQ) if baseline value less than (\<) LLOQ or missing or defined as post-baseline value \>3-fold increase from baseline if baseline value greater than or equal to (\>=) LLOQ.

    Time frame: Week 28

  22. Main Study: Percentage of Responders for Envelope (Env)-Specific Binding Antibody Titers at Week 52

    Percentage of responders for envelop (Env) Clade B (SC42261), Clade C (CH505TF), Clade A (9004S), Clade B (RHPA), Clade B (WITO), Clade C (1086C), Clade C (BF1266), CladeAE (conAE), Clade M(Con S)-specific binding antibody titers assessed using enzyme-linked immunosorbent assay (ELISA) were reported. The response was defined as post-baseline value greater than (\>) lower limit of quantification (LLOQ) if baseline value less than (\<) LLOQ or missing or defined as post-baseline value \>3-fold increase from baseline if baseline value greater than or equal to (\>=) LLOQ.

    Time frame: Week 52

  23. Main Study: Percentage of Responders for Envelope (Env)-Specific Binding Antibody Titers at Week 72

    Percentage of responders for envelop (Env) Clade B (SC42261), Clade C (CH505TF), Clade A (9004S), Clade B (RHPA), Clade B (WITO), Clade C (1086C), Clade C (BF1266), CladeAE (conAE), Clade M(Con S)-specific binding antibody titers assessed using enzyme-linked immunosorbent assay (ELISA) were reported. The response was defined as post-baseline value greater than (\>) lower limit of quantification (LLOQ) if baseline value less than (\<) LLOQ or missing or defined as post-baseline value \>3-fold increase from baseline if baseline value greater than or equal to (\>=) LLOQ.

    Time frame: Week 72

  24. Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 192

    Geometric mean of Env Mos 1 specific binding Abs response at Week 192 were assessed using ELISA.

    Time frame: Week 192

  25. Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 193

    Geometric Mean of Env Mos 1 specific binding Abs response at Week 193 were assessed using ELISA.

    Time frame: Week 193

  26. Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 196

    Geometric mean of Env Mos 1 specific binding Abs response at Week 196 were assessed using ELISA.

    Time frame: Week 196

  27. Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 204

    Geometric mean of Env Mos 1 specific binding Abs response at Week 204 were assessed using ELISA.

    Time frame: Week 204

  28. Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 216

    Geometric mean of Env Mos 1 specific binding Abs response at Week 216 were assessed using ELISA.

    Time frame: Week 216

  29. Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 240

    Geometric mean of Env Mos 1 specific binding Abs response at Week 240 were assessed using ELISA.

    Time frame: Week 240

  30. Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 288

    Geometric mean of Env Mos 1 specific binding Abs response at Week 288 were assessed using ELISA.

    Time frame: Week 288

Secondary outcomes

  1. Main Study: Percentage of Responders of Env-Specific Neutralizing Antibody (nAbs) for Tier 1 Viruses

    Percentage of responders of Env-specific nAbs for tier 1 viruses were reported. Viruses with a Tier 1 neutralization phenotype: Clade C: MW965 and 97ZA012, ZM233M, CE703010010, 2759058, ZM215F, SO431, CE704810053 were used. The response was defined as post-baseline value \>LLOQ.

    Time frame: Weeks 28, 52, and 72 (only for Clade C [MW965])

  2. Long-term Extension (LTE) Phase: Percentage of Responders of Env-Specific Neutralizing Antibody (nAbs) for Tier 1 Viruses

    Percentage of responders of Env-specific nAbs for tier 1 viruses were planned to be reported.

    Time frame: From Week 72 to Week 216

  3. Late-boost (LB) Vaccination Phase: Percentage of Responders of Env-Specific Neutralizing Antibody (nAbs) for Tier 1 Viruses

    Percentage of responders of Env-specific nAbs for tier 1 viruses were planned to be reported.

    Time frame: From Week 188 up to end of study (Week 288)

  4. Main Study: Percentage of Responders for Env-Specific Functional Antibody Response (Env ADCP gp140)

    Percentage of responders for Env-specific functional antibody response (Env ADCP gp140) were reported. The response was defined as post-baseline value \> limit of detection (LOD) if baseline value \<LOD or missing or defined as post-baseline value \>3-fold increase from baseline if baseline value \>=LOD. The lower limits of detection (LODs) for this assay were 5.16, 6.43, 6.49, 4.32 and 4.28 (phagocytic score) for Clade A (92UG037.1), Clade B (1990a), Clade C (Con C), Clade C (ZA), and Mos1, respectively.

    Time frame: Weeks 28, 52, and 72 (Weeks 52 and 72 are only for HIV ENV [gp140 T sortA] C [ZA] F Ab)

  5. Long-term Extension (LTE) Phase: Percentage of Responders for Env-Specific Functional Antibody Response (Env ADCP gp140)

    Percentage of responders for Env-specific functional antibody response (Env ADCP gp140) were planned to be reported.

    Time frame: From Week 72 up to Week 216

  6. Late-boost (LB) Vaccination Phase: Percentage of Responders for Env-Specific Functional Antibody Response (Env ADCP gp140)

    Percentage of responders for Env-specific functional antibody response (Env ADCP gp140) were planned to be reported.

    Time frame: From Week 188 up to end of study (Week 288)

  7. Main Study: Percentage of Responders for Env-Specific Binding Ab Isotypes (Immunoglobulin [Ig] G1 and IgG3)

    Percentage of responders for Env-specific binding Ab isotypes (IgG1 and IgG3) for Clade C (ZA) as assessed using ELISA were reported. The response was defined as post-baseline value \>LLOQ if baseline \<LLOQ or missing or defined as post-baseline value \>3-fold increase from baseline if baseline \>=LLOQ. The LLOQs for this assay were 12.3 and 12.4 EC50 for IgG1 and IgG3, respectively. EC50= 50% effective concentration.

    Time frame: Weeks 28, 52, and 72

  8. Long-term Extension (LTE) Phase: Percentage of Responders for Env-Specific Binding Ab Isotypes (Immunoglobulin [Ig] G1 and IgG3)

    Percentage of responders for Env-specific binding Ab isotypes IgG1 and IgG3) were planned to be reported.

    Time frame: From Week 72 up to Week 216

  9. Late-boost (LB) Vaccination Phase: Percentage of Responders for Env-Specific Binding Ab Isotypes (Immunoglobulin [Ig] G1 and IgG3)

    Percentage of responders for Env-specific binding Ab isotypes IgG1 and IgG3) were planned to be reported.

    Time frame: From Week 188 up to end of study (Week 288)

  10. Main Study: Percentage of Interferon (IFN)-Gamma Peripheral Blood Mononuclear Cell (PBMC) Responders to Mosaic and Potential T-cell Epitope (PTE) Peptide Pools of Env/Group-Specific Antigen (Gag)/ Polymerase (Pol)

    Percentage of IFN-gamma PBMC responders to mosaic and PTE peptide pools of Env/Gag/Pol as assessed by ELISpot was reported. The response was defined as post-baseline value \>P95 if baseline \<P95 or missing or defined as post-baseline value \>3-fold increase from baseline if baseline \>=P95.

    Time frame: Weeks 28, 52, and 72

  11. Long-term Extension (LTE) Phase: Percentage of Interferon (IFN)-Gamma Peripheral Blood Mononuclear Cell (PBMC) Responders to Mosaic and Potential T-cell Epitope (PTE) Peptide Pools of Env/Group-Specific Antigen (Gag)/ Polymerase (Pol)

    Percentage of Interferon (IFN)-Gamma PBMC responders to Mosaic and Potential T-cell Epitope (PTE) Peptide Pools of Env/Group-Specific Antigen (Gag)/ Polymerase (Pol) was planned to be reported.

    Time frame: From Week 72 up to Week 216

  12. Late-boost (LB) Vaccination Phase: Percentage of Interferon (IFN)-Gamma Peripheral Blood Mononuclear Cell (PBMC) Responders to Mosaic and Potential T-cell Epitope (PTE) Peptide Pools of Env/Group-Specific Antigen (Gag)/ Polymerase (Pol)

    Percentage of Interferon (IFN)-Gamma PBMC responders to Mosaic and Potential T-cell Epitope (PTE) Peptide Pools of Env/Group-Specific Antigen (Gag)/ Polymerase (Pol) were planned to be reported.

    Time frame: From Week 188 up to end of study (Week 288)

  13. Main Study: Percentage of Responders With Cluster of Differentiation (CD)4+ and CD8+ T-Cell Functionality

    Percentage of responders with CD4+ and CD8+ T-cell functionality (cells producing IFN-gamma and /or IL-2) were reported. Intracellular cytokine staining (ICS) was performed to examine the type of T-cell responding to vaccination. Responder definition was based on the Fisher's exact text between cytokine producing cells and non-producing cells in stimulated versus non-stimulated conditions.

    Time frame: Weeks 28, 52, and 72

  14. Long-term Extension (LTE) Phase: Percentage of Responders With Cluster of Differentiation (CD)4+ and CD8+ T-Cell Functionality

    Percentage of Responders With Cluster of Differentiation (CD)4+ and CD8+ T-Cell Functionality (cells Producing IFN-Gamma and/or Interleukin \[IL-2\]) were planned to be reported. Intracellular cytokine staining (ICS) was performed to examine the type of T-cell responding to vaccination. Responder definition was based on the Fisher's exact text between cytokine producing cells and non-producing cells in stimulated versus non-stimulated conditions.

    Time frame: From Week 72 up to Week 216

  15. Late-boost (LB) Vaccination Phase: Percentage of Responders With Cluster of Differentiation (CD)4+ and CD8+ T-Cell Functionality

    Percentage of responders with CD4+ and CD8+ T-cell functionality (cells producing IFN-gamma and /or IL-2) were reported. Intracellular cytokine staining (ICS) was performed to examine the type of T-cell responding to vaccination. Responder definition was based on the Fisher's exact text between cytokine producing cells and non-producing cells in stimulated versus non-stimulated conditions.

    Time frame: Weeks 192 and 196

  16. Main Study: Percentage of Participants With T-Cell Development

    Percentage of participants with T-Cell development were planned to be reported.

    Time frame: From Baseline (Day 1) up to Week 72

  17. Long-term Extension (LTE) Phase: Percentage of Participants With T-Cell Development

    Percentage of participants with T-Cell development were planned to be reported.

    Time frame: From Week 72 up to Week 216

  18. Late-boost (LB) Vaccination Phase: Percentage of Participants With T-Cell Development

    Percentage of participants with T-Cell Development were planned to be reported.

    Time frame: From Week 188 up to end of study (Week 288)

06

Results

Posted Jan 7, 2025
Limitations and caveats
Due to change in planned analysis, safety data for main study and LTE were combined for Groups 1 and 2 as LTE was a follow up for the participants who were in main study in Groups 1 and 2 and no intervention was given in LTE. As both phases involved the same participants in continued monitoring, the data reflects ongoing safety assessment.

Participant flow

Main Study (Week 0 up to Week 72)
Participant flow — Main Study (Week 0 up to Week 72)
MilestoneGroup 1 Main Study and LTE Phase: Ad26.Mos4.HIV + Clade C gp140Group 2 Main Study and LTE Phase: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3 Main Study: PlaceboGroup 1b: LTE Then Late-boost Ad26.Mos4.HIV + gp140 HIV Bivalent VaccineGroup 2b: LTE Then Late-boost Placebo
Started261032600
Vaccinated261002600
Completed19872400
Not completed716200
Withdrew: Adverse event10000
Withdrew: Lost to follow-up41200
Withdrew: Pregnancy02000
Withdrew: Withdrawal by subject29000
Withdrew: Other01000
Withdrew: Randomized but not vaccinated03000
Long-term Extension (Week 72 - 216)
Participant flow — Long-term Extension (Week 72 - 216)
MilestoneGroup 1 Main Study and LTE Phase: Ad26.Mos4.HIV + Clade C gp140Group 2 Main Study and LTE Phase: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3 Main Study: PlaceboGroup 1b: LTE Then Late-boost Ad26.Mos4.HIV + gp140 HIV Bivalent VaccineGroup 2b: LTE Then Late-boost Placebo
Started1476000
Participants entering lte and did not receive late-boost (weeks 72-216)333000
Completed1263000
Not completed213000
Withdrew: Lost to follow-up28000
Withdrew: Withdrawal by subject04000
Withdrew: Adverse event01000
Late Boost Vaccination (Week 192 - 288)
Participant flow — Late Boost Vaccination (Week 192 - 288)
MilestoneGroup 1 Main Study and LTE Phase: Ad26.Mos4.HIV + Clade C gp140Group 2 Main Study and LTE Phase: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3 Main Study: PlaceboGroup 1b: LTE Then Late-boost Ad26.Mos4.HIV + gp140 HIV Bivalent VaccineGroup 2b: LTE Then Late-boost Placebo
Started0004113
Completed0003911
Not completed00022
Withdrew: Adverse event00001
Withdrew: Lost to follow-up00021

Outcome measures

PrimaryMain Study: Number of Participants With Solicited Local and Systemic Adverse Events (AEs) for 7 Days Post-vaccination 1

Number of participants with solicited local and systemic AEs for 7 days post-vaccination 1 were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment. Solicited AEs were precisely local and systemic events for which the participant is specifically questioned and symptoms of which were noted by participant in their diary. Solicited local AEs included injection site pain/tenderness, erythema, and swelling/induration at the study vaccine injection site. Solicited systemic AEs included fever (temperature measurement), fatigue, headache, nausea, myalgia, and chills.

Time frame:
Up to 7 days post-vaccination 1 on Day 1 (up to Day 8)
Reported as:
Count of participants · Participants
Main Study: Number of Participants With Solicited Local and Systemic Adverse Events (AEs) for 7 Days Post-vaccination 1
ParticipantsGroup 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
Solicited local AE21786
Solicited systemic AE208016
PrimaryMain Study: Number of Participants With Solicited Local and Systemic Adverse Events (AEs) for 7 Days Post-vaccination 2

Number of participants with solicited local and systemic AEs for 7 days post-vaccination 2 were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment. Solicited AEs were precisely local and systemic events for which the participant is specifically questioned and symptoms of which were noted by participant in their diary. Solicited local AEs included injection site pain/tenderness, erythema, and swelling/induration at the study vaccine injection site. Solicited systemic AEs included fever (temperature measurement), fatigue, headache, nausea, myalgia, and chills.

Time frame:
Up to 7 days post vaccination 2 (up to any day from Day 78 to Day 113) (vaccination 2 ranged from Day 78 to 106)
Reported as:
Count of participants · Participants
Main Study: Number of Participants With Solicited Local and Systemic Adverse Events (AEs) for 7 Days Post-vaccination 2
ParticipantsGroup 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
Solicited local AE19665
Solicited systemic AE155813
PrimaryMain Study: Number of Participants With Solicited Local and Systemic Adverse Events (AEs) for 7 Days Post-vaccination 3

Number of participants with solicited local and systemic AEs for 7 days post-vaccination 3 were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment. Solicited AEs were precisely local and systemic events for which the participant is specifically questioned and symptoms of which were noted by participant in their diary. Solicited local AEs included injection site pain/tenderness, erythema, and swelling/induration at the study vaccine injection site. Solicited systemic AEs included fever (temperature measurement), fatigue, headache, nausea, myalgia, and chills.

Time frame:
Up to 7 days post vaccination 3 (up to any day from Day 162 to Day 197) (vaccination 3 ranged from Day 162 to 190)
Reported as:
Count of participants · Participants
Main Study: Number of Participants With Solicited Local and Systemic Adverse Events (AEs) for 7 Days Post-vaccination 3
ParticipantsGroup 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
Solicited local AE187312
Solicited systemic AE10558
PrimaryMain Study: Number of Participants With Solicited Local and Systemic Adverse Events (AEs) for 7 Days Post-vaccination 4

Number of participants with solicited local and systemic AEs for 7 days post-vaccination 4 were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment. Solicited AEs were precisely local and systemic events for which the participant is specifically questioned and symptoms of which were noted by participant in their diary. Solicited local AEs included injection site pain/tenderness, erythema, and swelling/induration at the study vaccine injection site. Solicited systemic AEs included fever (temperature measurement), fatigue, headache, nausea, myalgia, and chills.

Time frame:
Up to 7 days post vaccination 4 (up to any day from Day 330 to Day 365) (vaccination 4 ranged from Day 330 to 358)
Reported as:
Count of participants · Participants
Main Study: Number of Participants With Solicited Local and Systemic Adverse Events (AEs) for 7 Days Post-vaccination 4
ParticipantsGroup 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
Solicited local AE13707
Solicited systemic AE11536
PrimaryMain Study: Number of Participants With Unsolicited Adverse Events (AEs) for 28 Days Post-vaccination 1

Number of participants with unsolicited AEs for 28 days post-vaccination 1 were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment. Unsolicited AEs were all AEs for which the participant is not specifically questioned in the participant diary.

Time frame:
Up to 28 days post-vaccination 1 on Day 1 (Up to Day 29)
Reported as:
Count of participants · Participants
Main Study: Number of Participants With Unsolicited Adverse Events (AEs) for 28 Days Post-vaccination 1
ParticipantsGroup 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
Main Study: Number of Participants With Unsolicited Adverse Events (AEs) for 28 Days Post-vaccination 184012
PrimaryMain Study: Number of Participants With Unsolicited Adverse Events (AEs) for 28 Days Post-vaccination 2

Number of participants with unsolicited AEs for 28 days post-vaccination 2 were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment. Unsolicited AEs were all AEs for which the participant is not specifically questioned in the participant diary.

Time frame:
Up to 28 days post vaccination 2 (up to any day from Day 78 to Day 134) (vaccination 2 ranged from Day 78 to 106)
Reported as:
Count of participants · Participants
Main Study: Number of Participants With Unsolicited Adverse Events (AEs) for 28 Days Post-vaccination 2
ParticipantsGroup 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
Main Study: Number of Participants With Unsolicited Adverse Events (AEs) for 28 Days Post-vaccination 26357
PrimaryMain Study: Number of Participants With Unsolicited Adverse Events (AEs) for 28 Days Post-vaccination 3

Number of participants with unsolicited AEs for 28 days post-vaccination 3 were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment. Unsolicited AEs were all AEs for which the participant is not specifically questioned in the participant diary.

Time frame:
Up to 28 days post vaccination 3 (up to any day from Day 162 to Day 218) (vaccination 3 ranged from Day 162 to 190)
Reported as:
Count of participants · Participants
Main Study: Number of Participants With Unsolicited Adverse Events (AEs) for 28 Days Post-vaccination 3
ParticipantsGroup 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
Main Study: Number of Participants With Unsolicited Adverse Events (AEs) for 28 Days Post-vaccination 35388
PrimaryMain Study: Number of Participants With Unsolicited Adverse Events (AEs) for 28 Days Post-vaccination 4

Number of participants with unsolicited AEs for 28 days post-vaccination 4 were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment. Unsolicited AEs were all AEs for which the participant is not specifically questioned in the participant diary.

Time frame:
Up to 28 days post vaccination 4 (up to any day from Day 330 to Day 358) (vaccination 4 ranged from Day 330 to 358)
Reported as:
Count of participants · Participants
Main Study: Number of Participants With Unsolicited Adverse Events (AEs) for 28 Days Post-vaccination 4
ParticipantsGroup 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
Main Study: Number of Participants With Unsolicited Adverse Events (AEs) for 28 Days Post-vaccination 43305
PrimaryMain Study: Number of Participants Who Discontinued Study Vaccination Due to AEs

Number of participants who discontinued study vaccination due to AEs were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment.

Time frame:
From Baseline (Day 1) up to Week 72
Reported as:
Count of participants · Participants
Main Study: Number of Participants Who Discontinued Study Vaccination Due to AEs
ParticipantsGroup 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
Main Study: Number of Participants Who Discontinued Study Vaccination Due to AEs100
PrimaryMain Study: Number of Participants With Serious Adverse Events (SAEs)

Number of participants with SAEs were reported. An AE was any untoward medical occurrence in a clinical study administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the study vaccine. An SAE is an AE resulting in any of the following outcomes or deemed significant for any other reason: death; initial or prolonged inpatient hospitalization; life-threatening experience (immediate risk of dying); persistent or significant disability/incapacity; congenital anomaly/birth defect; suspected transmission of any infectious agent via a medicinal product or medically important.

Time frame:
From Baseline (Day 1) up to Week 72
Reported as:
Count of participants · Participants
Main Study: Number of Participants With Serious Adverse Events (SAEs)
ParticipantsGroup 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
Main Study: Number of Participants With Serious Adverse Events (SAEs)000
PrimaryMain Study and LTE Study: Number of Participants With Adverse Events of Special Interest (AESIs)

Number of participants with adverse events of special interest (AESIs) were reported. As planned, confirmed HIV infection was the only event assessed as an AESI. AESIs (including potential AESIs) are significant AEs that are judged to be of special interest because of clinical importance, known or suspected class effects, or based on nonclinical signals. AESIs (including potential AESIs) must be reported to the sponsor within 24 hours of awareness irrespective of seriousness (that is, serious and nonserious AEs) or causality.

Time frame:
From Baseline (Day 1) up to Week 216
Reported as:
Count of participants · Participants
Main Study and LTE Study: Number of Participants With Adverse Events of Special Interest (AESIs)
ParticipantsGroup 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3 Main Study: Placebo
Main Study and LTE Study: Number of Participants With Adverse Events of Special Interest (AESIs)000
PrimaryLate-boost (LB) Vaccination Phase: Number of Participants Who Discontinued Study Due to AEs

Number of participants who discontinued study due to AEs were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment.

Time frame:
From Week 188 up to end of study (Week 288)
Reported as:
Count of participants · Participants
Late-boost (LB) Vaccination Phase: Number of Participants Who Discontinued Study Due to AEs
ParticipantsGroup 1b: LTE Then Late-boost Ad26.Mos4.HIV + gp140 HIV Bivalent VaccineGroup 2b: LTE Then Late-boost Placebo
Late-boost (LB) Vaccination Phase: Number of Participants Who Discontinued Study Due to AEs01
PrimaryLate-boost (LB) Vaccination Phase: Number of Participants With Solicited Local and Systemic Adverse Events (AEs) for 7 Days Post Late Boost Vaccination

Number of participants with solicited local and systemic AEs for 7 days post late boost vaccination were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment. Solicited AEs were precisely local and systemic events for which the participant is specifically questioned and symptoms of which were noted by participant in their diary. Solicited local AEs included injection site pain/tenderness, erythema, and swelling/induration at the study vaccine injection site. Solicited systemic AEs included fever (temperature measurement), fatigue, headache, nausea, myalgia, and chills.

Time frame:
Up to 7 days post late boost vaccination (up to any day from Day 1317 to Day 1464) (late boost vaccination ranged from Day 1317 to 1457)
Reported as:
Count of participants · Participants
Late-boost (LB) Vaccination Phase: Number of Participants With Solicited Local and Systemic Adverse Events (AEs) for 7 Days Post Late Boost Vaccination
ParticipantsGroup 1b: LTE Then Late-boost Ad26.Mos4.HIV + gp140 HIV Bivalent VaccineGroup 2b: LTE Then Late-boost Placebo
Late-boost (LB) Vaccination Phase: Number of Participants With Solicited Local and Systemic Adverse Events (AEs) for 7 Days Post Late Boost Vaccination379
PrimaryLate-boost (LB) Vaccination Phase: Number of Participants With Unsolicited Adverse Events (AEs) for 28 Days Post Late Boost Vaccination

Number of participants with unsolicited AEs for 28 post late boost vaccination were reported. An AE is any untoward medical occurrence in a clinical study participant administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the treatment. Unsolicited AEs were all AEs for which the participant is not specifically questioned in the participant diary.

Time frame:
Up to 28 days post late boost vaccination (up to any day from Day 1317 to Day 1485) (late boost vaccination ranged from Day 1317 to 1457)
Reported as:
Count of participants · Participants
Late-boost (LB) Vaccination Phase: Number of Participants With Unsolicited Adverse Events (AEs) for 28 Days Post Late Boost Vaccination
ParticipantsGroup 1b: LTE Then Late-boost Ad26.Mos4.HIV + gp140 HIV Bivalent VaccineGroup 2b: LTE Then Late-boost Placebo
Late-boost (LB) Vaccination Phase: Number of Participants With Unsolicited Adverse Events (AEs) for 28 Days Post Late Boost Vaccination114
PrimaryLate-boost (LB) Vaccination Phase: Number of Participants With Serious Adverse Events (SAEs)

Number of participants with SAEs were reported. An AE was any untoward medical occurrence in a clinical study administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the study vaccine. An SAE is an AE resulting in any of the following outcomes or deemed significant for any other reason: death; initial or prolonged inpatient hospitalization; life-threatening experience (immediate risk of dying); persistent or significant disability/incapacity; congenital anomaly/birth defect; suspected transmission of any infectious agent via a medicinal product or medically important.

Time frame:
From Week 188 up to end of study (Week 288)
Reported as:
Count of participants · Participants
Late-boost (LB) Vaccination Phase: Number of Participants With Serious Adverse Events (SAEs)
ParticipantsGroup 1b: LTE Then Late-boost Ad26.Mos4.HIV + gp140 HIV Bivalent VaccineGroup 2b: LTE Then Late-boost Placebo
Late-boost (LB) Vaccination Phase: Number of Participants With Serious Adverse Events (SAEs)11
PrimaryLate-boost (LB) Vaccination Phase: Number of Participants With AESIs of HIV Infection Up to End of Study

Number of participants with AESIs up to the end of the study were reported. An AE was any untoward medical occurrence in a clinical study administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the study vaccine. AESIs (including potential AESIs) are significant AEs that are judged to be of special interest because of clinical importance, known or suspected class effects, or based on nonclinical signals. AESIs (including potential AESIs) must be reported to the sponsor within 24 hours of awareness irrespective of seriousness (i.e, serious and nonserious AEs) or causality. Confirmed HIV infection was considered an AESI.

Time frame:
From Week 188 up to end of study (Week 288)
Reported as:
Count of participants · Participants
Late-boost (LB) Vaccination Phase: Number of Participants With AESIs of HIV Infection Up to End of Study
ParticipantsGroup 1b: LTE Then Late-boost Ad26.Mos4.HIV + gp140 HIV Bivalent VaccineGroup 2b: LTE Then Late-boost Placebo
Late-boost (LB) Vaccination Phase: Number of Participants With AESIs of HIV Infection Up to End of Study02
PrimaryLate-boost (LB) Vaccination Phase: Number of Participants With AESIs of Thrombosis With Thrombocytopenia Syndrome (TTS)

Number of participants with AESIs of TTS were reported. An AE was any untoward medical occurrence in a clinical study administered a medicinal (investigational or non-investigational) product. An AE does not necessarily have a causal relationship with the study vaccine. AESIs are significant AEs that are judged to be of special interest because of clinical importance, known or suspected class effects, or based on nonclinical signals. AESIs must be reported to the sponsor within 24 hours of awareness irrespective of seriousness (i.e, serious and nonserious AEs) or causality. Thrombotic events and/or thrombocytopenia were considered as AESIs.

Time frame:
Up to 6 months post late boost vaccination (up to any day from Day 1317 to Day 1639) (late boost vaccination ranged from Day 1317 to 1457)
Reported as:
Count of participants · Participants
Late-boost (LB) Vaccination Phase: Number of Participants With AESIs of Thrombosis With Thrombocytopenia Syndrome (TTS)
ParticipantsGroup 1b: LTE Then Late-boost Ad26.Mos4.HIV + gp140 HIV Bivalent VaccineGroup 2b: LTE Then Late-boost Placebo
Late-boost (LB) Vaccination Phase: Number of Participants With AESIs of Thrombosis With Thrombocytopenia Syndrome (TTS)00
PrimaryMain Study: Geometric Mean of Envelope (Env) Clade A (92UG037.1), B (1990a), C (Con C), (C97ZA.012), Mos 1 Specific Binding Antibodies (Abs) Responses As Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 28

Geometric mean of Env Clade A (92UG037.1), B (1990a), C (Con C), (C97ZA.012), Mos 1 specific binding Abs responses were assessed using ELISA.

Time frame:
Week 28
Reported as:
Geometric mean · ELISA units/milliliter (EU/mL)
Main Study: Geometric Mean of Envelope (Env) Clade A (92UG037.1), B (1990a), C (Con C), (C97ZA.012), Mos 1 Specific Binding Antibodies (Abs) Responses As Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 28
ELISA units/milliliter (EU/mL)Group 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
Clade A (92UG037.1)99731.9 (61923.3 to 160625.5)88412.7 (7592.1 to 102917.5)312.5 (312.5 to 312.5)
Clade B (1990a)67190.3 (42923 to 105177.6)71067.3 (60104.7 to 84029.4)94.4 (71.8 to 123.9)
Clade C (Con C)149924.9 (91195.3 to 246476.1)130235.2 (110383.5 to 153656.9)333.3 (303.9 to 365.5)
Clade C (C97ZA.012)65644.1 (43866.4 to 98233.5)54942.6 (46038 to 65569.4)98.4 (70.7 to 136.9)
Mos 169234.2 (40940.5 to 117081.3)73780 (60679.2 to 89709.3)39.1 (39.1 to 39.1)
PrimaryMain Study: Geometric Mean of Envelope (Env) Clade A (92UG037.1), B (1990a), C (Con C), (C97ZA.012), Mos 1 Specific Binding Antibodies (Abs) Responses As Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 52

Geometric mean of Env Clade A (92UG037.1), B (1990a), C (Con C), (C97ZA.012), Mos 1 specific binding Abs responses were assessed using ELISA.

Time frame:
Week 52
Reported as:
Geometric mean · ELISA units/milliliter (EU/mL)
Main Study: Geometric Mean of Envelope (Env) Clade A (92UG037.1), B (1990a), C (Con C), (C97ZA.012), Mos 1 Specific Binding Antibodies (Abs) Responses As Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 52
ELISA units/milliliter (EU/mL)Group 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
Clade A (92UG037.1)104042.2 (78120.7 to 138564.8)139725.1 (117861.4 to 165644.6)312.5 (312.5 to 312.5)
Clade B (1990a)77042.3 (51359.9 to 115567.2)133424.3 (111903.8 to 159083.5)94.3 (72 to 123.5)
Clade C (Con C)155117.3 (107852.7 to 223094.6)237501.1 (197847.5 to 285102.3)323.1 (301.5 to 346.2)
Clade C (C97ZA.012)92936.2 (60391.6 to 143018.7)110083.7 (92455 to 131073.8)78.1 (78.1 to 78.1)
Mos 179595 (49206 to 128751.7)137520.1 (115722.2 to 163423.8)39.1 (39.1 to 39.1)
PrimaryMain Study: Geometric Mean of Envelope (Env) Clade A (92UG037.1) B (1990a), C (Con C), (C97ZA.012), Mos 1 Specific Binding Antibodies (Abs) Responses As Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 72

Geometric mean of Env Clade A (92UG037.1) B (1990a), C (Con C), (C97ZA.012), Mos 1 specific binding Abs responses were assessed using ELISA.

Time frame:
Week 72
Reported as:
Geometric mean · ELISA units/milliliter (EU/mL)
Main Study: Geometric Mean of Envelope (Env) Clade A (92UG037.1) B (1990a), C (Con C), (C97ZA.012), Mos 1 Specific Binding Antibodies (Abs) Responses As Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 72
ELISA units/milliliter (EU/mL)Group 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
Clade A (92UG037.1)33049.4 (22287.3 to 49008.4)39174.4 (32083 to 47833.2)312.5 (312.5 to 312.5)
Clade B (1990a)20625 (14107.1 to 30154.3)33177.5 (27444.9 to 40107.6)99.1 (69.7 to 140.8)
Clade C (Con C)44505.6 (31448.4 to 62984.1)57596.5 (47597.1 to 69696.7)342 (300.4 to 389.4)
Clade C (C97ZA.012)18236.1 (11989.7 to 27736.7)18857.2 (15759.6 to 22563.7)83.1 (73 to 94.6)
Mos 116861.6 (11096.6 to 25621.5)25161.6 (20879.1 to 30322.6)40.9 (37.2 to 44.9)
PrimaryMain Study: Percentage of Responders for Envelope (Env)-Specific Binding Antibody Titers at Week 28

Percentage of responders for envelop (Env) Clade B (SC42261), Clade C (CH505TF), Clade A (9004S), Clade B (RHPA), Clade B (WITO), Clade C (1086C), Clade C (BF1266), CladeAE (conAE), Clade M(Con S)-specific binding antibody titers assessed using enzyme-linked immunosorbent assay (ELISA) were reported. The response was defined as post-baseline value greater than (\>) lower limit of quantification (LLOQ) if baseline value less than (\<) LLOQ or missing or defined as post-baseline value \>3-fold increase from baseline if baseline value greater than or equal to (\>=) LLOQ.

Time frame:
Week 28
Reported as:
Number · percentage of responders
Main Study: Percentage of Responders for Envelope (Env)-Specific Binding Antibody Titers at Week 28
percentage of respondersGroup 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
Clade B (SC42261)100 (82.4 to 100)100.0 (95.6 to 100.0)0 (0 to 0)
Clade C (CH505TF)100 (82.4 to 100)100 (95.6 to 100)0 (0 to 0)
Clade A (9004S)100 (82.4 to 100)100 (95.6 to 100)0 (0 to 0)
Clade B (RHPA)100 (82.4 to 100)100 (95.6 to 100)0 (0 to 0)
Clade B (WITO)100 (82.4 to 100)100 (95.6 to 100)0 (0 to 0)
Clade C (1086C)100 (82.4 to 100)100 (95.6 to 100)0 (0 to 0)
Clade C (BF1266)100 (82.4 to 100)100 (95.6 to 100)0 (0 to 0)
CladeAE (conAE)100 (82.4 to 100)100 (95.6 to 100)0 (0 to 0)
Clade M(Con S)100 (82.4 to 100)100 (95.6 to 100)0 (0 to 0)
PrimaryMain Study: Percentage of Responders for Envelope (Env)-Specific Binding Antibody Titers at Week 52

Percentage of responders for envelop (Env) Clade B (SC42261), Clade C (CH505TF), Clade A (9004S), Clade B (RHPA), Clade B (WITO), Clade C (1086C), Clade C (BF1266), CladeAE (conAE), Clade M(Con S)-specific binding antibody titers assessed using enzyme-linked immunosorbent assay (ELISA) were reported. The response was defined as post-baseline value greater than (\>) lower limit of quantification (LLOQ) if baseline value less than (\<) LLOQ or missing or defined as post-baseline value \>3-fold increase from baseline if baseline value greater than or equal to (\>=) LLOQ.

Time frame:
Week 52
Reported as:
Number · percentage of responders
Main Study: Percentage of Responders for Envelope (Env)-Specific Binding Antibody Titers at Week 52
percentage of respondersGroup 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
Clade B (SC42261)100 (78.2 to 100)100.0 (95.2 to 100.0)0 (0 to 0)
Clade C (CH505TF)100 (78.2 to 100)100 (95.2 to 100)0 (0 to 0)
Clade A (9004S)100 (78.2 to 100)100 (95.2 to 100)0 (0 to 0)
Clade B (RHPA)100 (78.2 to 100)100 (95.2 to 100)0 (0 to 0)
Clade B (WITO)100 (78.2 to 100)100 (95.2 to 100)0 (0 to 0)
Clade C (1086C)100 (78.2 to 100)100 (95.2 to 100)0 (0 to 0)
Clade C (BF1266)100 (78.2 to 100)100 (95.2 to 100)0 (0 to 0)
CladeAE (conAE)100 (78.2 to 100)100 (95.2 to 100)0 (0 to 0)
Clade M(Con S)100 (78.2 to 100)100 (95.2 to 100)0 (0 to 0)
PrimaryMain Study: Percentage of Responders for Envelope (Env)-Specific Binding Antibody Titers at Week 72

Percentage of responders for envelop (Env) Clade B (SC42261), Clade C (CH505TF), Clade A (9004S), Clade B (RHPA), Clade B (WITO), Clade C (1086C), Clade C (BF1266), CladeAE (conAE), Clade M(Con S)-specific binding antibody titers assessed using enzyme-linked immunosorbent assay (ELISA) were reported. The response was defined as post-baseline value greater than (\>) lower limit of quantification (LLOQ) if baseline value less than (\<) LLOQ or missing or defined as post-baseline value \>3-fold increase from baseline if baseline value greater than or equal to (\>=) LLOQ.

Time frame:
Week 72
Reported as:
Number · percentage of responders
Main Study: Percentage of Responders for Envelope (Env)-Specific Binding Antibody Titers at Week 72
percentage of respondersGroup 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
Clade B (SC42261)93.8 (69.8 to 99.8)94.7 (87.1 to 98.5)0 (0 to 0)
Clade C (CH505TF)100 (78.2 to 100)96.1 (89 to 99.2)0 (0 to 0)
Clade A (9004S)100 (78.2 to 100)100 (95.2 to 100)0 (0 to 0)
Clade B (RHPA)100 (78.2 to 100)100 (95.3 to 100)0 (0 to 0)
Clade B (WITO)100 (78.2 to 100)100 (95.3 to 100)0 (0 to 0)
Clade C (1086C)100 (78.2 to 100.0)100 (95.3 to 100.0)0 (0 to 0)
Clade C (BF1266)100 (78.2 to 100.0)98.7 (93 to 100.0)0 (0 to 0)
CladeAE (conAE)100 (78.2 to 100)97.4 (90.9 to 99.7)0 (0 to 0)
Clade M(Con S)100 (78.2 to 100)100 (95.3 to 100)0 (0 to 0)
PrimaryLate-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 192

Geometric mean of Env Mos 1 specific binding Abs response at Week 192 were assessed using ELISA.

Time frame:
Week 192
Reported as:
Geometric mean · ELISA units/milliliter (EU/mL)
Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 192
ELISA units/milliliter (EU/mL)Group 1b:LTE Then LB:Ad26.Mos4.HIV+gp140 HIV Bivalent Vaccine (Previously in Group 1 of Main Study)Group 2b: LTE Then LB: Placebo (Previously in Group 1 of Main Study)Group 1b:LTE Then LB:Ad26.Mos4.HIV+gp140 HIV Bivalent Vaccine (Previously in Group 2 of Main Study)Group 2b: LTE Then LB: Placebo (Previously in Group 2 of Main Study)
Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 1924044.7 (2132.9 to 7670.2)3398.9 (979.0 to 11800.9)6816.4 (5125.4 to 9065.2)5125.0 (3079.4 to 8529.3)
PrimaryLate-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 193

Geometric Mean of Env Mos 1 specific binding Abs response at Week 193 were assessed using ELISA.

Time frame:
Week 193
Reported as:
Geometric mean · ELISA units/milliliter (EU/mL)
Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 193
ELISA units/milliliter (EU/mL)Group 1b:LTE Then LB:Ad26.Mos4.HIV+gp140 HIV Bivalent Vaccine (Previously in Group 1 of Main Study)Group 2b: LTE Then LB: Placebo (Previously in Group 1 of Main Study)Group 1b:LTE Then LB:Ad26.Mos4.HIV+gp140 HIV Bivalent Vaccine (Previously in Group 2 of Main Study)Group 2b: LTE Then LB: Placebo (Previously in Group 2 of Main Study)
Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 19328412.2 (7194.6 to 112202.7)3652.9 (7.7 to 1726909.7)46851.6 (26104.6 to 84087.6)4505.3 (3439.9 to 5900.6)
PrimaryLate-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 196

Geometric mean of Env Mos 1 specific binding Abs response at Week 196 were assessed using ELISA.

Time frame:
Week 196
Reported as:
Geometric mean · ELISA units/milliliter (EU/mL)
Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 196
ELISA units/milliliter (EU/mL)Group 1b: LTE Then LB:Ad26.Mos4.HIV+gp140 HIV Bivalent Vaccine (Previously in Group 1 of Main Study)Group 2b: LTE Then LB: Placebo (Previously in Group 1 of Main Study)Group 1b: LTE Then LB:Ad26.Mos4.HIV+gp140 HIV Bivalent Vaccine (Previously in Group 2 of Main Study)Group 2b: LTE Then LB: Placebo (Previously in Group 2 of Main Study)
Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 196169017.3 (102848.8 to 277755.8)3438.1 (1190.8 to 9926.6)174004.6 (124094.4 to 243988.4)5153.9 (3377.6 to 7864.4)
PrimaryLate-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 204

Geometric mean of Env Mos 1 specific binding Abs response at Week 204 were assessed using ELISA.

Time frame:
Week 204
Reported as:
Geometric mean · ELISA units/milliliter (EU/mL)
Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 204
ELISA units/milliliter (EU/mL)Group 1b:LTE Then LB: Ad26.Mos4.HIV+gp140 HIV Bivalent Vaccine (Previously in Group 1 of Main Study)Group 2b: LTE Then LB: Placebo (Previously in Group 1 of Main Study)Group 1b:LTE Then LB: Ad26.Mos4.HIV+gp140 HIV Bivalent Vaccine (Previously in Group 2 of Main Study)Group 2b: LTE Then LB: Placebo (Previously in Group 2 of Main Study)
Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 20465259.8 (35974.8 to 118383.9)3440.8 (835.5 to 14170.2)74715.2 (51288.6 to 108842.1)5303.2 (3435.7 to 8185.7)
PrimaryLate-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 216

Geometric mean of Env Mos 1 specific binding Abs response at Week 216 were assessed using ELISA.

Time frame:
Week 216
Reported as:
Geometric mean · ELISA units/milliliter (EU/mL)
Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 216
ELISA units/milliliter (EU/mL)Group 1b:LTE Then LB: Ad26.Mos4.HIV+gp140 HIV Bivalent Vaccine (Previously in Group 1 of Main Study)Group 2b: LTE Then LB: Placebo (Previously in Group 1 of Main Study)Group 1b: LTE Then LB:Ad26.Mos4.HIV+gp140 HIV Bivalent Vaccine (Previously in Group 2 of Main Study)Group 2b: LTE Then LB: Placebo (Previously in Group 2 of Main Study)
Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 21627307.4 (12695.8 to 58735.4)3237.3 (4.2 to 2511983.7)43831.8 (30033.5 to 63969.3)5029.6 (3219.4 to 7857.7)
PrimaryLate-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 240

Geometric mean of Env Mos 1 specific binding Abs response at Week 240 were assessed using ELISA.

Time frame:
Week 240
Reported as:
Geometric mean · ELISA units/milliliter (EU/mL)
Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 240
ELISA units/milliliter (EU/mL)Group 1b: LTE Then LB:Ad26.Mos4.HIV+gp140 HIV Bivalent Vaccine (Previously in Group 1 of Main Study)Group 2b: LTE Then LB: Placebo (Previously in Group 1 of Main Study)Group 1b:LTE Then LB: Ad26.Mos4.HIV+gp140 HIV Bivalent Vaccine (Previously in Group 2 of Main Study)Group 2b: LTE Then LB: Placebo (Previously in Group 2 of Main Study)
Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 24018223.4 (8647.7 to 38402.3)3031.1 (19.6 to 467682.9)25693.2 (17757.9 to 37174.7)4491.1 (2492.1 to 8093.7)
PrimaryLate-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 288

Geometric mean of Env Mos 1 specific binding Abs response at Week 288 were assessed using ELISA.

Time frame:
Week 288
Reported as:
Geometric mean · ELISA units/milliliter (EU/mL)
Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 288
ELISA units/milliliter (EU/mL)Group 1b: LTE Then LB:Ad26.Mos4.HIV+gp140 HIV Bivalent Vaccine (Previously in Group 1 of Main Study)Group 2b: LTE Then LB: Placebo (Previously in Group 1 of Main Study)Group 1b: LTE Then LB:Ad26.Mos4.HIV+gp140 HIV Bivalent Vaccine (Previously in Group 2 of Main Study)Group 2b: LTE Then LB: Placebo (Previously in Group 2 of Main Study)
Late-boost (LB) Vaccination Phase: Geometric Mean of Envelope (Env) Mos 1 Specific Binding Antibodies (Abs) Response as Assessed Using Enzyme-linked Immunosorbent Assay (ELISA) at Week 28818799.1 (8267.1 to 42748.8)4742.3 (NA to NA)15757.0 (10438.7 to 23784.7)3933.1 (2772.2 to 5580.2)
SecondaryMain Study: Percentage of Responders of Env-Specific Neutralizing Antibody (nAbs) for Tier 1 Viruses

Percentage of responders of Env-specific nAbs for tier 1 viruses were reported. Viruses with a Tier 1 neutralization phenotype: Clade C: MW965 and 97ZA012, ZM233M, CE703010010, 2759058, ZM215F, SO431, CE704810053 were used. The response was defined as post-baseline value \>LLOQ.

Time frame:
Weeks 28, 52, and 72 (only for Clade C [MW965])
Reported as:
Number · Percentage of responders
Main Study: Percentage of Responders of Env-Specific Neutralizing Antibody (nAbs) for Tier 1 Viruses
Percentage of respondersGroup 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
Clade C (MW965): Week 28100.0 (83.2 to 100.0)100.0 (96.0 to 100.0)0 (0 to 0)
Clade C (MW965): Week 52100.0 (80.5 to 100.0)100.0 (95.5 to 100.0)0 (0 to 0)
Clade C (MW965) Week 72100.0 (80.5 to 100.0)98.7 (93.1 to 100.0)—
Clade C (97ZA012): Week 280 (0 to 0)0 (0 to 0)0 (0 to 0)
Clade C (97ZA012): Week 520 (0 to 0)0 (0 to 0)0 (0 to 0)
Clade C (ZM233M): Week 280 (0 to 0)0 (0 to 0)—
Clade C (ZM233M): Week 520 (0 to 0)0 (0 to 0)—
Clade C (CE703010010): Week 280 (0 to 0)0 (0 to 0)—
Clade C (CE703010010): Week 520 (0 to 0)0 (0 to 0)—
Clade C (2759058): Week 280 (0 to 0)0 (0 to 0)—
Clade C (2759058): Week 520 (0 to 0)0 (0 to 0)—
Clade C (ZM215F): Week 280 (0 to 0)0 (0 to 0)—
Clade C (ZM215F): Week 520 (0 to 0)0 (0 to 0)—
Clade C (SO431): Week 280 (0 to 0)0 (0 to 0)—
Clade C (SO431): Week 520 (0 to 0)0 (0 to 0)—
Clade C (CE704810053): Week 280 (0 to 0)0 (0 to 0)—
Clade C(CE704810053): Week 520 (0 to 0)0 (0 to 0)—
SecondaryLong-term Extension (LTE) Phase: Percentage of Responders of Env-Specific Neutralizing Antibody (nAbs) for Tier 1 Viruses

Percentage of responders of Env-specific nAbs for tier 1 viruses were planned to be reported.

Time frame:
From Week 72 to Week 216

No measurements were reported for this outcome.

SecondaryLate-boost (LB) Vaccination Phase: Percentage of Responders of Env-Specific Neutralizing Antibody (nAbs) for Tier 1 Viruses

Percentage of responders of Env-specific nAbs for tier 1 viruses were planned to be reported.

Time frame:
From Week 188 up to end of study (Week 288)

No measurements were reported for this outcome.

SecondaryMain Study: Percentage of Responders for Env-Specific Functional Antibody Response (Env ADCP gp140)

Percentage of responders for Env-specific functional antibody response (Env ADCP gp140) were reported. The response was defined as post-baseline value \> limit of detection (LOD) if baseline value \<LOD or missing or defined as post-baseline value \>3-fold increase from baseline if baseline value \>=LOD. The lower limits of detection (LODs) for this assay were 5.16, 6.43, 6.49, 4.32 and 4.28 (phagocytic score) for Clade A (92UG037.1), Clade B (1990a), Clade C (Con C), Clade C (ZA), and Mos1, respectively.

Time frame:
Weeks 28, 52, and 72 (Weeks 52 and 72 are only for HIV ENV [gp140 T sortA] C [ZA] F Ab)
Reported as:
Number · Percentage of Responders
Main Study: Percentage of Responders for Env-Specific Functional Antibody Response (Env ADCP gp140)
Percentage of RespondersGroup 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
HIV ENV (gp140 M sortA) A (92UG037) F Ab: Week 2890.094.432.0
HIV ENV (gp140 M sortA) B (1990a) F Ab: Week 2890.098.90
HIV ENV (gp140 M sortA) C (conC) F Ab: Week 2885.091.10
HIV ENV (gp140 T sortA) (Mos1) F Ab: Week 28100.0100.036.0
HIV ENV (gp140 T sortA) C (ZA) F Ab: Week 2895.0100.08.0
HIV ENV (gp140 T sortA) C (ZA) F Ab: Week 52100.0100.013.0
HIV ENV (gp140 T sortA) C (ZA) F Ab: Week 72100.096.452.4
SecondaryLong-term Extension (LTE) Phase: Percentage of Responders for Env-Specific Functional Antibody Response (Env ADCP gp140)

Percentage of responders for Env-specific functional antibody response (Env ADCP gp140) were planned to be reported.

Time frame:
From Week 72 up to Week 216

No measurements were reported for this outcome.

SecondaryLate-boost (LB) Vaccination Phase: Percentage of Responders for Env-Specific Functional Antibody Response (Env ADCP gp140)

Percentage of responders for Env-specific functional antibody response (Env ADCP gp140) were planned to be reported.

Time frame:
From Week 188 up to end of study (Week 288)

No measurements were reported for this outcome.

SecondaryMain Study: Percentage of Responders for Env-Specific Binding Ab Isotypes (Immunoglobulin [Ig] G1 and IgG3)

Percentage of responders for Env-specific binding Ab isotypes (IgG1 and IgG3) for Clade C (ZA) as assessed using ELISA were reported. The response was defined as post-baseline value \>LLOQ if baseline \<LLOQ or missing or defined as post-baseline value \>3-fold increase from baseline if baseline \>=LLOQ. The LLOQs for this assay were 12.3 and 12.4 EC50 for IgG1 and IgG3, respectively. EC50= 50% effective concentration.

Time frame:
Weeks 28, 52, and 72
Reported as:
Number · Percentage of Responders
Main Study: Percentage of Responders for Env-Specific Binding Ab Isotypes (Immunoglobulin [Ig] G1 and IgG3)
Percentage of RespondersGroup 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
HIV ENV (gp140 T) clade C (ZA) IgG-1 Ab: Week 2865.0 (40.8 to 84.6)76.7 (66.6 to 84.9)0 (0 to 0)
HIV ENV (gp140 T) clade C (ZA) IgG-1 Ab: Week 5276.5 (50.1 to 93.2)75.6 (64.9 to 84.4)0 (0 to 0)
HIV ENV (gp140 T) clade C (ZA) IgG-1 Ab: Week 7276.5 (50.1 to 93.2)66.3 (55.1 to 76.3)0 (0 to 0)
HIV ENV (gp140 T) clade C (ZA) IgG-3 Ab: Week 2860.0 (36.1 to 80.9)75.0 (64.6 to 83.6)8.7 (1.1 to 28.0)
HIV ENV (gp140 T) clade C (ZA) IgG-3 Ab: Week 5275.0 (47.6 to 92.7)73.2 (62.2 to 82.4)0 (0 to 0)
HIV ENV (gp140 T) clade C (ZA) IgG-3 Ab: Week 7223.5 (6.8 to 49.9)36.6 (26.2 to 48.0)14.3 (3.0 to 36.3)
SecondaryLong-term Extension (LTE) Phase: Percentage of Responders for Env-Specific Binding Ab Isotypes (Immunoglobulin [Ig] G1 and IgG3)

Percentage of responders for Env-specific binding Ab isotypes IgG1 and IgG3) were planned to be reported.

Time frame:
From Week 72 up to Week 216

No measurements were reported for this outcome.

SecondaryLate-boost (LB) Vaccination Phase: Percentage of Responders for Env-Specific Binding Ab Isotypes (Immunoglobulin [Ig] G1 and IgG3)

Percentage of responders for Env-specific binding Ab isotypes IgG1 and IgG3) were planned to be reported.

Time frame:
From Week 188 up to end of study (Week 288)

No measurements were reported for this outcome.

SecondaryMain Study: Percentage of Interferon (IFN)-Gamma Peripheral Blood Mononuclear Cell (PBMC) Responders to Mosaic and Potential T-cell Epitope (PTE) Peptide Pools of Env/Group-Specific Antigen (Gag)/ Polymerase (Pol)

Percentage of IFN-gamma PBMC responders to mosaic and PTE peptide pools of Env/Gag/Pol as assessed by ELISpot was reported. The response was defined as post-baseline value \>P95 if baseline \<P95 or missing or defined as post-baseline value \>3-fold increase from baseline if baseline \>=P95.

Time frame:
Weeks 28, 52, and 72
Reported as:
Number · Percentage of Responders
Main Study: Percentage of Interferon (IFN)-Gamma Peripheral Blood Mononuclear Cell (PBMC) Responders to Mosaic and Potential T-cell Epitope (PTE) Peptide Pools of Env/Group-Specific Antigen (Gag)/ Polymerase (Pol)
Percentage of RespondersGroup 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
HIV IFNg ENV pep pool (Clinical PTE): Week 2890.5 (69.6 to 98.8)82.0 (72.5 to 89.4)0 (0 to 0)
HIV IFNg ENV pep pool (Clinical PTE): Week 5282.4 (56.6 to 96.2)87.8 (78.7 to 94.0)4.3 (0.1 to 21.9)
HIV IFNg ENV pep pool (Clinical PTE): Week 7276.5 (50.1 to 93.2)84.3 (74.7 to 91.4)4.8 (0.1 to 23.8)
HIV IFNg ENV pep pool (Mos1): Week 2890.5 (69.6 to 98.8)85.4 (76.3 to 92.0)4.0 (0.1 to 20.4)
HIV IFNg ENV pep pool (Mos1): Week 5294.1 (71.3 to 99.9)84.1 (74.4 to 91.3)13.0 (2.8 to 33.6)
HIV IFNg ENV pep pool (Mos1): Week 7285.7 (57.2 to 98.2)85.9 (76.2 to 92.7)10.0 (1.2 to 31.7)
HIV IFNg ENV pep pool (Mos2): Week 2881.0 (58.1 to 94.6)88.8 (80.3 to 94.5)4.0 (0.1 to 20.4)
HIV IFNg ENV pep pool (Mos2): Week 5294.1 (71.3 to 99.9)87.8 (78.7 to 94.0)8.7 (1.1 to 28.0)
HIV IFNg ENV pep pool (Mos2): Week 7270.6 (44.0 to 89.7)86.7 (77.5 to 93.2)4.8 (0.1 to 23.8)
HIV IFNg Gag pep pool (Clinical PTE) : Week 2852.4 (29.8 to 74.3)31.5 (22.0 to 42.2)0 (0 to 0)
HIV IFNg Gag pep pool (Clinical PTE) : Week 5241.2 (18.4 to 67.1)32.9 (22.9 to 44.2)0 (0 to 0)
HIV IFNg Gag pep pool (Clinical PTE) : Week 7241.2 (18.4 to 67.1)26.5 (17.4 to 37.3)0 (0 to 0)
HIV IFNg Gag pep pool (Mos1): Week 2852.4 (29.8 to 74.3)56.2 (45.3 to 66.7)4.0 (0.1 to 20.4)
HIV IFNg Gag pep pool (Mos1): Week 5252.9 (27.8 to 77.0)51.2 (39.9 to 62.4)4.3 (0.1 to 21.9)
HIV IFNg Gag pep pool (Mos1): Week 7252.9 (27.8 to 77.0)48.2 (37.1 to 59.4)4.8 (0.1 to 23.8)
HIV IFNg Gag pep pool (Mos2): Week 2861.9 (38.4 to 81.9)60.7 (49.7 to 70.9)8.0 (1.0 to 26.0)
HIV IFNg Gag pep pool (Mos2): Week 5275.0 (47.6 to 92.7)52.4 (41.1 to 63.6)8.7 (1.1 to 28.0)
HIV IFNg Gag pep pool (Mos2): Week 7258.8 (32.9 to 81.6)47.0 (35.9 to 58.3)0 (0 to 0)
HIV IFNg Pol pep pool (Clinical PTE): Week 2876.2 (52.8 to 91.8)77.5 (67.4 to 85.7)4.0 (0.1 to 20.4)
HIV IFNg Pol pep pool (Clinical PTE): Week 5282.4 (56.6 to 96.2)86.6 (77.3 to 93.1)4.3 (0.1 to 21.9)
HIV IFNg Pol pep pool (Clinical PTE): Week 7276.5 (50.1 to 93.2)83.1 (73.3 to 90.5)0 (0 to 0)
HIV IFNg Pol pep pool (Mos1): Week 2876.2 (52.8 to 91.8)75.3 (65.0 to 83.8)8.0 (1.0 to 26.0)
HIV IFNg Pol pep pool (Mos1): Week 5258.8 (32.9 to 81.6)78.0 (67.5 to 86.4)17.4 (5.0 to 38.8)
HIV IFNg Pol pep pool (Mos1): Week 7247.1 (23.0 to 72.2)73.5 (62.7 to 82.6)0 (0 to 0)
HIV IFNg Pol pep pool (Mos2): Week 2876.2 (52.8 to 91.8)83.1 (73.7 to 90.2)4.0 (0.1 to 20.4)
HIV IFNg Pol pep pool (Mos2): Week 5275.0 (47.6 to 92.7)85.4 (75.8 to 92.2)17.4 (5.0 to 38.8)
HIV IFNg Pol pep pool (Mos2): Week 7270.6 (44.0 to 89.7)80.7 (70.6 to 88.6)9.5 (1.2 to 30.4)
SecondaryLong-term Extension (LTE) Phase: Percentage of Interferon (IFN)-Gamma Peripheral Blood Mononuclear Cell (PBMC) Responders to Mosaic and Potential T-cell Epitope (PTE) Peptide Pools of Env/Group-Specific Antigen (Gag)/ Polymerase (Pol)

Percentage of Interferon (IFN)-Gamma PBMC responders to Mosaic and Potential T-cell Epitope (PTE) Peptide Pools of Env/Group-Specific Antigen (Gag)/ Polymerase (Pol) was planned to be reported.

Time frame:
From Week 72 up to Week 216

No measurements were reported for this outcome.

SecondaryLate-boost (LB) Vaccination Phase: Percentage of Interferon (IFN)-Gamma Peripheral Blood Mononuclear Cell (PBMC) Responders to Mosaic and Potential T-cell Epitope (PTE) Peptide Pools of Env/Group-Specific Antigen (Gag)/ Polymerase (Pol)

Percentage of Interferon (IFN)-Gamma PBMC responders to Mosaic and Potential T-cell Epitope (PTE) Peptide Pools of Env/Group-Specific Antigen (Gag)/ Polymerase (Pol) were planned to be reported.

Time frame:
From Week 188 up to end of study (Week 288)

No measurements were reported for this outcome.

SecondaryMain Study: Percentage of Responders With Cluster of Differentiation (CD)4+ and CD8+ T-Cell Functionality

Percentage of responders with CD4+ and CD8+ T-cell functionality (cells producing IFN-gamma and /or IL-2) were reported. Intracellular cytokine staining (ICS) was performed to examine the type of T-cell responding to vaccination. Responder definition was based on the Fisher's exact text between cytokine producing cells and non-producing cells in stimulated versus non-stimulated conditions.

Time frame:
Weeks 28, 52, and 72
Reported as:
Number · Percentage of responders
Main Study: Percentage of Responders With Cluster of Differentiation (CD)4+ and CD8+ T-Cell Functionality
Percentage of respondersGroup 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3: Placebo
CD4+: HIV ENV pep pool (Mos1 ZA) IFNg+ or IL2+ (%): Week 2850.00 (27.20 to 72.80)80.95 (70.92 to 88.70)0 (0 to 0)
CD4+: HIV ENV pep pool (Mos1 ZA) IFNg+ or IL2+ (%): Week 5264.29 (35.14 to 87.24)77.46 (66.00 to 86.54)0 (0 to 0)
CD4+: HIV ENV pep pool (Mos1 ZA) IFNg+ or IL2+ (%): Week 7241.18 (18.44 to 67.08)69.23 (57.76 to 79.19)0 (0 to 0)
CD4+: HIV ENV pep pool (Mos1) IFNg+ or IL2+ (%): Week 2850.00 (27.20 to 72.80)80.95 (70.92 to 88.70)0 (0 to 0)
CD4+: HIV ENV pep pool (Mos1) IFNg+ or IL2+ (%): Week 5264.29 (35.14 to 87.24)74.65 (62.92 to 84.23)0 (0 to 0)
CD4+: HIV ENV pep pool (Mos1) IFNg+ or IL2+ (%): Week 7229.41 (10.31 to 55.96)69.23 (57.76 to 79.19)0 (0 to 0)
CD4+: HIV ENV pep pool clade C (ZA) IFNg+ or IL2+ (%): Week 2840.00 (19.12 to 63.95)53.57 (42.35 to 64.53)0 (0 to 0)
CD4+: HIV ENV pep pool clade C (ZA) IFNg+ or IL2+ (%): Week 5257.14 (28.86 to 82.34)47.89 (35.88 to 60.08)0 (0 to 0)
CD4+: HIV ENV pep pool clade C (ZA) IFNg+ or IL2+ (%): Week 7229.41 (10.31 to 55.96)21.79 (13.24 to 32.59)0 (0 to 0)
CD4+: HIV Env Pol Gag pep pool IFNg+ or IL2+ (%): Week 2850.00 (27.20 to 72.80)82.14 (72.26 to 89.65)0 (0 to 0)
CD4+: HIV Env Pol Gag pep pool IFNg+ or IL2+ (%): Week 5264.29 (35.14 to 87.24)77.46 (66.00 to 86.54)0 (0 to 0)
CD4+: HIV Env Pol Gag pep pool IFNg+ or IL2+ (%): Week 7241.18 (18.44 to 67.08)69.23 (57.76 to 79.19)0 (0 to 0)
CD4+: HIV Gag pep pool ANY IFNg+ or IL2+ (%): Week 2810.00 (1.23 to 31.70)8.33 (3.42 to 16.42)0 (0 to 0)
CD4+: HIV Gag pep pool ANY: Week 527.14 (0.18 to 33.87)9.86 (4.06 to 19.26)0 (0 to 0)
CD4+: HIV Gag pep pool ANY IFNg+ or IL2+ (%): Week 725.88 (0.15 to 28.69)1.28 (0.03 to 6.94)0 (0 to 0)
CD4+: HIV Pol pep pool (Mos1) IFNg+ or IL2+ (%): Week 2810.00 (1.23 to 31.70)8.33 (3.42 to 16.42)0 (0 to 0)
CD4+: HIV Pol pep pool (Mos1) IFNg+ or IL2+ (%): Week 520 (0 to 0)11.27 (4.99 to 21.00)0 (0 to 0)
CD4+: HIV Pol pep pool (Mos1) IFNg+ or IL2+ (%): Week 720 (0 to 0)1.28 (0.03 to 6.94)0 (0 to 0)
CD4 +: HIV ENV gp120 pep pool 1 (Mos1) IFNg+ or IL2+ (%): Week 2850.00 (27.20 to 72.80)80.95 (70.92 to 88.70)0 (0 to 0)
CD4 +: HIV ENV gp120 pep pool 1 (Mos1) IFNg+ or IL2+ (%): Week 5257.14 (28.86 to 82.34)73.24 (61.41 to 83.06)0 (0 to 0)
CD4 +: HIV ENV gp120 pep pool 1 (Mos1) IFNg+ or IL2+ (%): Week 7229.41 (10.31 to 55.96)69.23 (57.76 to 79.19)0 (0 to 0)
CD4 +:HIV ENV gp120 pep pool clade C (ZA) IFNg+ or IL2+ (%): Week 2840.00 (19.12 to 63.95)47.62 (36.60 to 58.81)0 (0 to 0)
CD4 +:HIV ENV gp120 pep pool clade C (ZA) IFNg+ or IL2+ (%): Week 5242.86 (17.66 to 71.14)43.66 (31.91 to 55.95)0 (0 to 0)
CD4 +:HIV ENV gp120 pep pool clade C (ZA) IFNg+ or IL2+ (%): Week 7223.53 (6.81 to 49.90)19.23 (11.18 to 29.73)0 (0 to 0)
CD4 +:HIV ENV gp41 pep pool 1 (Mos1) IFNg+ or IL2+ (%): Week 2815.79 (3.38 to 39.58)28.05 (18.68 to 39.06)0 (0 to 0)
CD4 +:HIV ENV gp41 pep pool 1 (Mos1) IFNg+ or IL2+ (%): Week 5221.43 (4.66 to 50.80)19.72 (11.22 to 30.86)0 (0 to 0)
CD4 +:HIV ENV gp41 pep pool 1 (Mos1) IFNg+ or IL2+ (%): Week 725.88 (0.15 to 28.69)12.82 (6.32 to 22.32)0 (0 to 0)
CD4 +:HIV ENV gp41 pep pool clade C (ZA) IFNg+ or IL2+ (%): Week 2820.00 (5.73 to 43.66)32.14 (22.36 to 43.22)0 (0 to 0)
CD4 +:HIV ENV gp41 pep pool clade C (ZA) IFNg+ or IL2+ (%): Week 5242.86 (17.66 to 71.14)23.94 (14.61 to 35.54)0 (0 to 0)
CD4 +:HIV ENV gp41 pep pool clade C (ZA) IFNg+ or IL2+ (%): Week 725.88 (0.15 to 28.69)10.26 (4.53 to 19.21)0 (0 to 0)
CD4 +:HIV Gag pep pool (Mos1) IFNg+ or IL2+ (%): Week 2810.00 (1.23 to 31.70)8.33 (3.42 to 16.42)0 (0 to 0)
CD4 +:HIV Gag pep pool (Mos1) IFNg+ or IL2+ (%): Week 527.14 (0.18 to 33.87)9.86 (4.06 to 19.26)0 (0 to 0)
CD4 +:HIV Gag pep pool (Mos1) IFNg+ or IL2+ (%): Week 725.88 (0.15 to 28.69)1.28 (0.03 to 6.94)0 (0 to 0)
CD4 +:HIV Pol RNAseInt pep pool 1 (Mos1) IFNg+ or IL2+ (%): Week 2810.00 (1.23 to 31.70)2.38 (0.29 to 8.34)0 (0 to 0)
CD4 +:HIV Pol RNAseInt pep pool 1 (Mos1) IFNg+ or IL2+ (%): Week 520 (0 to 0)7.04 (2.33 to 15.67)0 (0 to 0)
CD4 +:HIV Pol RNAseInt pep pool 1 (Mos1) IFNg+ or IL2+ (%): Week 720 (0 to 0)1.28 (0.03 to 6.94)0 (0 to 0)
CD4 +:HIV Pol RT pep pool (Mos1) IFNg+ or IL2+ (%): Week 2810.00 (1.23 to 31.70)5.95 (1.96 to 13.35)0 (0 to 0)
CD4 +:HIV Pol RT pep pool (Mos1) IFNg+ or IL2+ (%): Week 520 (0 to 0)7.04 (2.33 to 15.67)0 (0 to 0)
CD4 +:HIV Pol RT pep pool (Mos1) IFNg+ or IL2+ (%): Week 720 (0 to 0)0 (0 to 0)0 (0 to 0)
CD8 +: HIV ENV pep pool (Mos1 ZA) IFNg+ or IL2+ (%): Week 2821.05 (6.05 to 45.57)33.33 (23.58 to 44.25)0 (0 to 0)
CD8+: HIV ENV pep pool (Mos1 ZA) IFNg+ or IL2+ (%): Week 5229.41 (10.31 to 55.96)29.11 (19.43 to 40.42)0 (0 to 0)
CD8 +: HIV ENV pep pool (Mos1 ZA) IFNg+ or IL2+ (%): Week 7217.65 (3.80 to 43.43)26.25 (17.04 to 37.29)0 (0 to 0)
CD8 +: HIV ENV pep pool (Mos1) IFNg+ or IL2+ (%): Week 2810.53 (1.30 to 33.14)29.89 (20.54 to 40.65)0 (0 to 0)
CD8 +: HIV ENV pep pool (Mos1) IFNg+ or IL2+ (%): Week 5211.76 (1.46 to 36.44)26.58 (17.27 to 37.72)0 (0 to 0)
CD8+: HIV ENV pep pool (Mos1) IFNg+ or IL2+ (%): Week 725.88 (0.15 to 28.69)25.00 (15.99 to 35.94)0 (0 to 0)
CD8 +: HIV ENV pep pool clade C (ZA) IFNg+ or IL2+ (%): Week 2815.79 (3.38 to 39.58)14.94 (8.20 to 24.20)0 (0 to 0)
CD8 +: HIV ENV pep pool clade C (ZA) IFNg+ or IL2+ (%): Week 5229.41 (10.31 to 55.96)13.92 (7.16 to 23.55)0 (0 to 0)
CD8 +: HIV ENV pep pool clade C (ZA) IFNg+ or IL2+ (%): Week 7217.65 (3.80 to 43.43)13.75 (7.07 to 23.27)0 (0 to 0)
CD8 +: HIV Env Pol Gag pep pool IFNg+ or IL2+ (%): Week 2873.68 (48.80 to 90.85)58.62 (47.55 to 69.08)0 (0 to 0)
CD8 +: HIV Env Pol Gag pep pool IFNg+ or IL2+ (%): Week 5270.59 (44.04 to 89.69)58.23 (46.59 to 69.23)0 (0 to 0)
CD8 +: HIV Env Pol Gag pep pool IFNg+ or IL2+ (%): Week 7252.94 (27.81 to 77.02)52.50 (41.02 to 63.79)0 (0 to 0)
CD8 +: HIV Gag pep pool ANY IFNg+ or IL2+ (%): Week 2836.84 (16.29 to 61.64)21.84 (13.69 to 31.98)0 (0 to 0)
CD8 +: HIV Gag pep pool ANY IFNg+ or IL2+ (%): Week 5235.29 (14.21 to 61.67)22.78 (14.10 to 33.60)0 (0 to 0)
CD8 +: HIV Gag pep pool ANY IFNg+ or IL2+ (%): Week 7229.41 (10.31 to 55.96)17.50 (9.91 to 27.62)0 (0 to 0)
CD8 +: HIV Pol pep pool (Mos1) IFNg+ or IL2+ (%): Week 2863.16 (38.36 to 83.71)47.13 (36.33 to 58.13)0 (0 to 0)
CD8 +: HIV Pol pep pool (Mos1) IFNg+ or IL2+ (%): Week 5258.82 (32.92 to 81.56)49.37 (37.92 to 60.86)0 (0 to 0)
CD8 +: HIV Pol pep pool (Mos1) IFNg+ or IL2+ (%): Week 7229.41 (10.31 to 55.96)40.00 (29.20 to 51.56)0 (0 to 0)
CD8 +:HIV ENV gp120 pep pool clade C (ZA) IFNg+ or IL2+ (%): Week 2815.79 (3.38 to 39.58)9.20 (4.05 to 17.32)0 (0 to 0)
CD8 +:HIV ENV gp120 pep pool clade C (ZA) IFNg+ or IL2+ (%): Week 5229.41 (10.31 to 55.96)10.13 (4.47 to 18.98)0 (0 to 0)
CD8 +:HIV ENV gp120 pep pool clade C (ZA) IFNg+ or IL2+ (%): Week 7217.65 (3.80 to 43.43)8.75 (3.59 to 17.20)0 (0 to 0)
CD8 +:HIV ENV gp41 pep pool 1 (Mos1) IFNg+ or IL2+ (%): Week 285.56 (0.14 to 27.29)11.76 (5.79 to 20.57)0 (0 to 0)
CD8 +:HIV ENV gp41 pep pool 1 (Mos1) IFNg+ or IL2+ (%): Week 520 (0 to 0)12.66 (6.24 to 22.05)0 (0 to 0)
CD8 +:HIV ENV gp41 pep pool 1 (Mos1) IFNg+ or IL2+ (%): Week 720 (0 to 0)13.75 (7.07 to 23.27)0 (0 to 0)
CD8 +:HIV ENV gp41 pep pool clade C (ZA) IFNg+ or IL2+ (%): Week 280 (0 to 0)9.20 (4.05 to 17.32)0 (0 to 0)
CD8 +:HIV ENV gp41 pep pool clade C (ZA) IFNg+ or IL2+ (%): Week 520 (0 to 0)6.33 (2.09 to 14.16)0 (0 to 0)
CD8 +:HIV ENV gp41 pep pool clade C (ZA) IFNg+ or IL2+ (%): Week 720 (0 to 0)7.50 (2.80 to 15.61)0 (0 to 0)
CD8 +:HIV Gag pep pool (Mos1) IFNg+ or IL2+ (%): Week 2836.84 (16.29 to 61.64)21.84 (13.69 to 31.98)0 (0 to 0)
CD8 +:HIV Gag pep pool (Mos1) IFNg+ or IL2+ (%): Week 5235.29 (14.21 to 61.67)22.78 (14.10 to 33.60)0 (0 to 0)
CD8 +:HIV Gag pep pool (Mos1) IFNg+ or IL2+ (%): Week 7229.41 (10.31 to 55.96)17.50 (9.91 to 27.62)0 (0 to 0)
CD8 +:HIV Pol RNAseInt pep pool 1 (Mos1) IFNg+ or IL2+ (%): Week 2857.89 (33.50 to 79.75)29.89 (20.54 to 40.65)0 (0 to 0)
CD8 +:HIV Pol RNAseInt pep pool 1 (Mos1) IFNg+ or IL2+ (%): Week 5247.06 (22.98 to 72.19)30.38 (20.53 to 41.75)0 (0 to 0)
CD8 +:HIV Pol RNAseInt pep pool 1 (Mos1) IFNg+ or IL2+ (%): Week 7217.65 (3.80 to 43.43)20.00 (11.89 to 30.44)0 (0 to 0)
CD8 +:HIV Pol RT pep pool (Mos1) IFNg+ or IL2+ (%): Week 2831.58 (12.58 to 56.55)31.03 (21.55 to 41.86)0 (0 to 0)
CD8 +:HIV Pol RT pep pool (Mos1) IFNg+ or IL2+ (%): Week 5229.41 (10.31 to 55.96)30.38 (20.53 to 41.75)0 (0 to 0)
CD8 +:HIV Pol RT pep pool (Mos1) IFNg+ or IL2+ (%): Week 7223.53 (6.81 to 49.90)27.50 (18.10 to 38.62)0 (0 to 0)
SecondaryLong-term Extension (LTE) Phase: Percentage of Responders With Cluster of Differentiation (CD)4+ and CD8+ T-Cell Functionality

Percentage of Responders With Cluster of Differentiation (CD)4+ and CD8+ T-Cell Functionality (cells Producing IFN-Gamma and/or Interleukin \[IL-2\]) were planned to be reported. Intracellular cytokine staining (ICS) was performed to examine the type of T-cell responding to vaccination. Responder definition was based on the Fisher's exact text between cytokine producing cells and non-producing cells in stimulated versus non-stimulated conditions.

Time frame:
From Week 72 up to Week 216

No measurements were reported for this outcome.

SecondaryLate-boost (LB) Vaccination Phase: Percentage of Responders With Cluster of Differentiation (CD)4+ and CD8+ T-Cell Functionality

Percentage of responders with CD4+ and CD8+ T-cell functionality (cells producing IFN-gamma and /or IL-2) were reported. Intracellular cytokine staining (ICS) was performed to examine the type of T-cell responding to vaccination. Responder definition was based on the Fisher's exact text between cytokine producing cells and non-producing cells in stimulated versus non-stimulated conditions.

Time frame:
Weeks 192 and 196
Reported as:
Number · Percentage of responders
Late-boost (LB) Vaccination Phase: Percentage of Responders With Cluster of Differentiation (CD)4+ and CD8+ T-Cell Functionality
Percentage of respondersGroup 1b: LTE Then Late-boost Ad26.Mos4.HIV + gp140 HIV Bivalent VaccineGroup 2b: LTE Then Late-boost Placebo
CD4 +:Any Env IFNg+ or IL2+ (%): Week 19282.6 (61.22 to 95.05)57.1 (18.41 to 90.10)
CD4 +:Any Env IFNg+ or IL2+ (%): Week 19691.3 (71.96 to 98.93)83.3 (35.88 to 99.58)
CD4 +:Any HIV IFNg+ or IL2+ (%): Week 19282.6 (61.22 to 95.05)57.1 (18.41 to 90.10)
CD4 +:Any HIV IFNg+ or IL2+ (%): Week 19691.3 (71.96 to 98.93)83.3 (35.88 to 99.58)
CD4 +:Any MOS1 Env IFNg+ or IL2+ (%): Week 19273.9 (51.59 to 89.77)57.1 (18.41 to 90.10)
CD4 +:Any MOS1 Env IFNg+ or IL2+ (%): Week 19691.3 (71.96 to 98.93)83.3 (35.88 to 99.58)
CD4 +:Any MOS2 Env IFNg+ or IL2+ (%): Week 19265.2 (42.73 to 83.62)42.9 (9.90 to 81.59)
CD4 +:Any MOS2 Env IFNg+ or IL2+ (%): Week 19673.9 (51.59 to 89.77)33.3 (4.33 to 77.72)
CD4 +:J Mos1 gp120 IFNg+ or IL2+ (%): Week 19273.9 (51.59 to 89.77)57.1 (18.41 to 90.10)
CD4 +:J Mos1 gp120 IFNg+ or IL2+ (%): Week 19691.3 (71.96 to 98.93)83.3 (35.88 to 99.58)
CD4 +:J Mos1 gp41 IFNg+ or IL2+ (%): Week 19239.1 (19.71 to 61.46)14.3 (0.36 to 57.87)
CD4 +:J Mos1 gp41 IFNg+ or IL2+ (%): Week 19643.5 (23.19 to 65.51)33.3 (4.33 to 77.72)
CD4 +:J Mos2 Gag IFNg+ or IL2+ (%): Week 19221.7 (7.46 to 43.70)0 (0 to 0)
CD4 +:J Mos2 Gag IFNg+ or IL2+ (%): Week 19639.1 (19.71 to 61.46)0 (0 to 0)
CD4 +:J Mos2 RNAseInt IFNg+ or IL2+ (%): Week 19217.4 (4.95 to 38.78)0 (0 to 0)
CD4 +:J Mos2 RNAseInt IFNg+ or IL2+ (%): Week 19617.4 (4.95 to 38.78)16.7 (0.42 to 64.12)
CD4 +:J Mos2S gp120 IFNg+ or IL2+ (%): Week 19260.9 (38.54 to 80.29)42.9 (9.90 to 81.59)
CD4 +:J Mos2S gp120 IFNg+ or IL2+ (%): Week 19669.6 (47.08 to 86.79)33.3 (4.33 to 77.72)
CD4 +:J Mos2S gp41 IFNg+ or IL2+ (%): Week 19221.7 (7.46 to 43.70)0 (0 to 0)
CD4 +:J Mos2S gp41 IFNg+ or IL2+ (%): Week 19643.5 (23.19 to 65.51)16.7 (0.42 to 64.12)
CD4 +: POSITIVE CONTROL (CMV) IFNg+ or IL2+ (%): Week 19252.2 (30.59 to 73.18)57.1 (18.41 to 90.10)
CD4 +: POSITIVE CONTROL (CMV) IFNg+ or IL2+ (%): Week 19650.0 (28.22 to 71.78)80.0 (28.36 to 99.49)
CD8 +: Any HIV IFNg+ or IL2+ (%): Week 19273.9 (51.59 to 89.77)28.6 (3.67 to 70.96)
CD8 +: Any HIV IFNg+ or IL2+ (%): Week 19673.9 (51.59 to 89.77)33.3 (4.33 to 77.72)
CD8 +: Any MOS1 Env IFNg+ or IL2+ (%): Week 19234.8 (16.38 to 57.27)14.3 (0.36 to 57.87)
CD8 +: Any MOS1 Env IFNg+ or IL2+ (%): Week 19643.5 (23.19 to 65.51)16.7 (0.42 to 64.12)
CD8 +: Any MOS2 Env IFNg+ or IL2+ (%): Week 19247.8 (26.82 to 69.41)14.3 (0.36 to 57.87)
CD8 +: Any MOS2 Env IFNg+ or IL2+ (%): Week 19647.8 (26.82 to 69.41)33.3 (4.33 to 77.72)
CD8 +: J Mos1 gp120 IFNg+ or IL2+ (%): Week 19217.4 (4.95 to 38.78)14.3 (0.36 to 57.87)
CD8 +: J Mos1 gp120 IFNg+ or IL2+ (%): Week 19626.1 (10.23 to 48.41)16.7 (0.42 to 64.12)
CD8 +: J Mos1 gp41 IFNg+ or IL2+ (%): Week 19217.4 (4.95 to 38.78)0 (0 to 0)
CD8 +: J Mos1 gp41 IFNg+ or IL2+ (%): Week 19621.7 (7.46 to 43.70)0 (0 to 0)
CD8 +: J Mos2 Gag IFNg+ or IL2+ (%): Week 19230.4 (13.21 to 52.92)0 (0 to 0)
CD8 +: J Mos2 Gag IFNg+ or IL2+ (%): Week 19630.4 (13.21 to 52.92)0 (0 to 0)
CD8 +: J Mos2 RNAseInt IFNg+ or IL2+ (%): Week 19234.8 (16.38 to 57.27)14.3 (0.36 to 57.87)
CD8 +: J Mos2 RNAseInt IFNg+ or IL2+ (%): Week 19634.8 (16.38 to 57.27)16.7 (0.42 to 64.12)
CD8 +: J Mos2S gp120 IFNg+ or IL2+ (%): Week 19230.4 (13.21 to 52.92)14.3 (0.36 to 57.87)
CD8 +: J Mos2S gp120 IFNg+ or IL2+ (%): Week 19634.8 (16.38 to 57.27)16.7 (0.42 to 64.12)
CD8 +: J Mos2S gp41 IFNg+ or IL2+ (%): Week 19217.4 (4.95 to 38.78)0 (0 to 0)
CD8 +: J Mos2S gp41 IFNg+ or IL2+ (%): Week 19617.4 (4.95 to 38.78)16.7 (0.42 to 64.12)
CD8 +: POSITIVE CONTROL (CMV) IFNg+ or IL2+ (%): Week 19252.2 (30.59 to 73.18)71.4 (29.04 to 96.33)
CD8 +: POSITIVE CONTROL (CMV) IFNg+ or IL2+ (%): Week 19650.0 (28.22 to 71.78)80.0 (28.36 to 99.49)
SecondaryMain Study: Percentage of Participants With T-Cell Development

Percentage of participants with T-Cell development were planned to be reported.

Time frame:
From Baseline (Day 1) up to Week 72

No measurements were reported for this outcome.

SecondaryLong-term Extension (LTE) Phase: Percentage of Participants With T-Cell Development

Percentage of participants with T-Cell development were planned to be reported.

Time frame:
From Week 72 up to Week 216

No measurements were reported for this outcome.

SecondaryLate-boost (LB) Vaccination Phase: Percentage of Participants With T-Cell Development

Percentage of participants with T-Cell Development were planned to be reported.

Time frame:
From Week 188 up to end of study (Week 288)

No measurements were reported for this outcome.

Adverse events

Collected over Group 1 and 2: Baseline up to Week 72 for participants who didn't enter LTE; up Week 216 for participants entering the LTE but not receiving late boost vaccination. Group 3: Baseline up to Week 72. Group 1b and 2b: From Week 188 up to Week 288.. Non-serious events are listed at a 5% frequency threshold.

Adverse event summary by group
GroupDeathsSeriousOther
Group 1 Main Study and LTE Phase: Ad26.Mos4.HIV + Clade C gp1400/26 (0%)1/26 (3.8%)25/26 (96.2%)
Group 2 Main Study and LTE Phase: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp1400/100 (0%)2/100 (2%)96/100 (96%)
Group 3 Main Study: Placebo0/26 (0%)0/26 (0%)25/26 (96.2%)
Group 1b: LTE Then Late-boost Ad26.Mos4.HIV + gp140 HIV Bivalent Vaccine0/41 (0%)1/41 (2.4%)38/41 (92.7%)
Group 2b: LTE Then Late-boost Placebo0/13 (0%)3/13 (23.1%)9/13 (69.2%)
Most frequent serious events
Most frequent serious events
EventGroup 1 Main Study and LTE Phase: Ad26.Mos4.HIV + Clade C gp140Group 2 Main Study and LTE Phase: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3 Main Study: PlaceboGroup 1b: LTE Then Late-boost Ad26.Mos4.HIV + gp140 HIV Bivalent VaccineGroup 2b: LTE Then Late-boost Placebo
CholecystitisHepatobiliary disorders0/260/1000/260/411/13
Tibia FractureInjury, poisoning and procedural complications0/260/1000/260/411/13
Abortion SpontaneousPregnancy, puerperium and perinatal conditions1/260/1000/260/411/13
Death NeonatalGeneral disorders0/260/1000/261/410/13
Foetal DeathPregnancy, puerperium and perinatal conditions0/260/1000/261/410/13
Miscarriage of PartnerSocial circumstances0/261/1000/261/410/13
GastritisGastrointestinal disorders0/261/1000/260/410/13
GastroenteritisInfections and infestations0/261/1000/260/410/13
Most frequent other events
Showing 10 of 23
Most frequent other events
EventGroup 1 Main Study and LTE Phase: Ad26.Mos4.HIV + Clade C gp140Group 2 Main Study and LTE Phase: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3 Main Study: PlaceboGroup 1b: LTE Then Late-boost Ad26.Mos4.HIV + gp140 HIV Bivalent VaccineGroup 2b: LTE Then Late-boost Placebo
Pain/Tenderness (Solicited)General disorders24/2694/10016/2634/414/13
Fatigue (Solicited)General disorders19/2678/10018/2626/417/13
Headache (Solicited)Nervous system disorders15/2669/10016/2620/413/13
Myalgia (Solicited)Musculoskeletal and connective tissue disorders15/2668/1009/2616/410/13
Chills (Solicited)General disorders12/2648/1001/265/413/13
Nausea (Solicited)Gastrointestinal disorders9/2646/1009/263/411/13
Upper Respiratory Tract InfectionInfections and infestations3/2616/1006/261/410/13
Aspartate Aminotransferase IncreasedInvestigations4/260/1001/260/410/13
Pyrexia (Solicited)General disorders2/2615/1001/261/410/13
Alanine Aminotransferase IncreasedInvestigations3/261/1001/260/410/13

Baseline characteristics

The Full Analysis Set (FA) consisted of all participants who were randomized and who received at least one dose of study vaccine.

Age, Continuous
Age, Continuous(years)Group 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3 Main Study: PlaceboTotal
Mean28.7 ± 7.0731.8 ± 8.0930.7 ± 10.1131.1 ± 8.34
Sex: Female, Male
Sex: Female, Male(Participants)Group 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3 Main Study: PlaceboTotal
Female16591590
Male10411162
Ethnicity (NIH/OMB)
Ethnicity (NIH/OMB)(Participants)Group 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3 Main Study: PlaceboTotal
Hispanic or Latino26412
Not Hispanic or Latino249222138
Unknown or Not Reported0202
Race/Ethnicity, Customized
Race/Ethnicity, Customized(Participants)Group 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3 Main Study: PlaceboTotal
Asian27211
Black or African American1038957
White12471271
Other28313
Region of Enrollment
Region of Enrollment(Participants)Group 1: Ad26.Mos4.HIV + Clade C gp140Group 2: Ad26.Mos4.HIV + Clade C gp140 + Mosaic gp140Group 3 Main Study: PlaceboTotal
KENYA1405
RWANDA726740
UNITED STATES187019107
07

Study locations

13 sites
  • Alabama Vaccine Research Clinic at UAB
    Birmingham, Alabama 35294, United States
  • Bridge HIV
    San Francisco, California 94102-4594, United States
  • The Hope Clinic at Emory University
    Decatur, Georgia 30030-1705, United States
  • Brigham And Women's Hospital
    Boston, Massachusetts 02115, United States
  • Beth Israel Deaconess Medical Center
    Boston, Massachusetts 02215, United States
  • Fenway Health
    Boston, Massachusetts 02215, United States
  • Columbia University HIV Vaccine Unit
    New York, New York 10032, United States
  • New York Blood Center
    New York, New York 10065, United States
  • Strong Memorial Infectious Disease
    Rochester, New York 14642, United States
  • University of Pennsylvania
    Philadelphia, Pennsylvania 19104, United States
  • Seattle Vaccine Trials Unit
    Seattle, Washington 98104, United States
  • Walter Reed Project Clinical Research Center
    Kericho, 20200, Kenya
  • Center for Family Health Research/Project San Francisco
    Kigali, 780, Rwanda
08

References and documents

Study documents

  • Study protocol · Oct 27, 2021
  • Statistical analysis plan · Mar 21, 2022

Documents are hosted by the registry — open the source record to download them.

09

Registry details

Key details

Study ID
NCT02935686
Lead sponsor
Janssen Vaccines & Prevention B.V.
Responsible party
Sponsor
First posted
Oct 17, 2016
Start date
Mar 31, 2017
Primary completion
Nov 22, 2023
Completion
Nov 22, 2023
Results posted
Jan 7, 2025
Last update
May 25, 2025

Study contacts

Janssen Vaccines & Prevention B.V. Clinical Trial
study director · Janssen Vaccines & Prevention B.V.

Oversight

Data monitoring committee
No
FDA-regulated drug
Yes
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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