An interventional study of embryos will be transferred using a medium of 0.5 mg/mL of Hyaluronan-enriched medium (EmbryoGlue®, Vitrolife, Sweden) and embryos will be transferred using a medium of 30% Protein-supplemented Culture Medium (Global total®, Life Global group, Canada) in Improvement of Embryo Transfer Technique and Improvement of Implantation, sponsored by South Valley University. Status unknown. Open to female participants aged 18 Years to 35 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2016-06-07.
Sponsored by South Valley University · Not applicable and Interventional
The study design is a prospective randomized uncontrolled clinical trial. It will include 150 cases.
The aim of this study is to compare the efficacy of three different media used for embryo transfer regarding "chemical and clinical" pregnancy rate, implantation rate and ongoing pregnancy and miscarriage rates in IVF and ICSI cycles.
The three different embryo transfer media are:
Two or three embryos will be transferred at day 5 after ovum pick-up (OPU).
The patients will be divided randomly (closed envelop method) into 3 groups:
Follicular fluid will be collected in a sterile procedure, centrifuged for 20 minutes in an embryo test tube. The supernatant will be removed in a sterilized technique then heated to 56°C for 30 minutes then filtered through 0.22 micron filter. Gentamycin sulphate will be added at a concentration of 10 µg/mL then the liquid is preserved in a normal freezer.
Defreezing follicular fluid will be performed at day-1 before embryo transfer. The follicular fluid will be warmed to room temperature. Preparation of embryo transfer media will be performed as usual, will be covered with paraffin oil (Ovoil®, Vitrolife, Sweeden) and then incubated overnight in 7% CO2, 9% O2, 22% N2.
All embryo transfers will be performed using an embryo transfer catheter (Labotect catheter, Labotect GmbH, Germany).
Number of embryos transferred will be according to ASRM guideline 2013 on day 5 after ovum pick-up (OPU).
South Valley University is the lead sponsor of 147 studies on the registry; 46 are open to participants now.
Counted across the registry records on this site, refreshed daily.
Exclusion Criteria:
Hyaluronan-enriched medium
Other: embryos will be transferred using a medium of 0.5 mg/mL of Hyaluronan-enriched medium (EmbryoGlue®, Vitrolife, Sweden) · Device: Labotect embryo transfer catheter
30% Protein-supplemented Culture Medium
Other: embryos will be transferred using a medium of 30% Protein-supplemented Culture Medium (Global total®, Life Global group, Canada) · Device: Labotect embryo transfer catheter
a novel technique
Other: a novel technique in which embryos will be transferred using a medium of autologous Follicular Fluid · Device: Labotect embryo transfer catheter
Chemical pregnancy rate
assessed by quantitative β hCG in the serum
Time frame: 14 days after embryo transfer
Clinical pregnancy rate
assessed by ultrasonic visualization of intrauterine gestational sac
Time frame: 6th week of gestation
Implantation rate.
Time frame: 6th week of gestation
Ongoing pregnancy and miscarriage rates
Time frame: 12th week of gestation
No study locations are listed for this record.
Plan to share: Undecided
No publications or documents are linked to this record.
This study is status unknown, as verified in Jun 2016. You cannot join it, but the record below documents what was studied.
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