An observational study in COPD, sponsored by Laval University. Status unknown. Open to participants aged 40 Years to 80 Years. Per ClinicalTrials.gov, last updated 2015-09-23.
Sponsored by Laval University · Observational
Muscle weakness and atrophy are important consequences of chronic obstructive pulmonary disease (COPD) and can affect both respiratory and peripheral muscles. Impaired muscle regeneration is thought to be implicated in the process of muscle atrophy. In this study the investigators will evaluate muscle regenerative potential of the Vastus Lateralis (VL) and diaphragm of patients with COPD, compared to patients with normal lung function.
Preliminary results suggest that the activation and differentiation processes of satellite cells isolated from the VL of patients with COPD could be altered. The investigators propose to compare the regenerative potential of the VL and the diaphragm in 15 patients with COPD and 15 individuals with normal lung function undergoing a thoracic surgery. From muscle biopsies, satellite cells will be isolated and cultured to assess their proliferation and differentiation rates, and the expression level of myogenic effectors (MyoD, Myf5, myogenin, and MRF4). Alterations in these measurements will confirm the hypotheses that the regenerative potential is lower in both muscle groups in patients with COPD compared to controls and that in COPD, the regenerative potential is lower in VL compared to the diaphragm.
Laval University is the lead sponsor of 371 studies on the registry; 68 are open to participants now.
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Patient with a scheduled thoracic surgery
Exclusion Criteria:
Patients with mild to moderate COPD having a scheduled lung surgery. Vastus lateralis and diaphragm muscle biopsies performed during surgery
Procedure: Muscle biopsies
Patients with normal lung function having a scheduled lung surgery. Vastus lateralis and diaphragm muscle biopsies performed during surgery.
Procedure: Muscle biopsies
Muscle biopsies will allow acces to samples for observational studies. Vastus latetalis: needle biopsy (Bergstrom technique). Diaphragm: open biopsy performed by thoracic surgeon during lung surgery.
Muscle satellite cell proliferation index
Isolation from muscle biopsies of satellite cell to evaluate their regeneration capacity by evaluating the proliferation capacity
Time frame: Sequential analyses performed over a period of 2 weeks after tissue collection.
Muscle satellite cell differentiation index
Isolation from muscle biopsies of satellite cell to evaluate their regeneration capacity by evaluating the differentiation capacity
Time frame: Sequential analyses performed over a period of 2 months after tissue collection.
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This study is status unknown, as verified in Sep 2015. You cannot join it, but the record below documents what was studied.
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