An observational study in Red Blood Cell, Peripheral Blood Mononuclear Cell and Endothelial NO-synthase, sponsored by Heinrich-Heine University, Duesseldorf. Completed at 1 site in Germany. Open to participants aged 20 Years to 75 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2021-02-16.
Sponsored by Heinrich-Heine University, Duesseldorf · Observational
The aim of this study is to investigate wether change in endothelial nitric oxid synthase of blood cells (erythrocytes and peripheral blood mononuclear cells (PBMC)) has an influence on development and progression of cardiovascular diseases.
Heinrich-Heine University, Duesseldorf is the lead sponsor of 174 studies on the registry; 52 are open to participants now.
Counted across the registry records on this site, refreshed daily.
100 healthy volunteers and 100 patient with stable coronary artery disease
Exclusion Criteria:
100 healthy volunteers in age of 20-75 years
100 patients with stable coronary artery disease and accordingly endothelial dysfunction
Blood examination
Following parameters will be examined: * Blood count (red cells, white cells, platelets, hemoglobin, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red blood cell distribution width (RDW), mean platelet volume (MPV) ) * Creatinin * Urea * Glucose * Glycated hemoglobin (HbA1c) * Cholesterol * Triglyceride * C-reactive Protein * Apolipoprotein
Time frame: baseline
Protein expression
Determination with western blot
Time frame: baseline
Asymmetric dimethylarginine (ADMA) and Cytokine
Determination with enzyme-linked immunosorbent assay (ELISA).
Time frame: baseline
Microparticles
Determination with ELISA and flow cytometry
Time frame: baseline
Immunocytochemistry
Time frame: baseline
Nitric oxid metabolite and proteins in blood plasma, red blood cells and peripheral blood mononuclear cells (PBMN)
Determination with reductive chemiluminescence
Time frame: baseline
Nitric oxid and reactive oxygen species
Determination with fluorescence coloring in flow cytometry and fluorescence microscopy
Time frame: baseline
This study is completed, as verified in Feb 2021. You cannot join it, but the record below documents what was studied.
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Heinrich-Heine University, Duesseldorf