An interventional study of Docosahexaenoic acid diet supplementation and Placebo supplementation in Oxidative Stress, Exercise and Training Season, sponsored by University of the Balearic Islands. Completed at 1 site in Spain. Open to male participants aged 16 Years to 35 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2014-06-27.
Sponsored by University of the Balearic Islands · Not applicable, Interventional, and Basic science
There are conflicting evidences of the effects of dietary supplementation with polyunsaturated fatty acids (PUFAs) on athletic performance. The investigators working hypothesis is based on the polyunsaturated fatty acids, given its pro-oxidative character and also by its action on transcription factors can modulate the antioxidant response and oxidative damage induced by physical activity.The overall objective of the study is to establish situations improving athletic performance and study the oxidative balance by manipulation of feeding patterns and consumption of nutritional supplements and investigate the involved mechanisms.
There are conflicting evidences of the effects of dietary supplementation with polyunsaturated fatty acids (PUFAs) on athletic performance. The described effects of PUFAs on the fat metabolism may be modulated by the expression and activity of transcription factor genes, suppressing or expressing genes associated with the synthesis and oxidation of fats and in oxidative stress and inflammation.
The investigators' working hypothesis is based on the polyunsaturated fatty acids, given its pro-oxidative character and also by its action on transcription factors can modulate the antioxidant response and oxidative damage induced by physical activity.
In the field of professional sports and leisure use of food and / or antioxidants in the belief that these elements prevent muscle damage is very established. Most studies in athletes supplemented with antioxidant nutrients revealed no significant effects on physical performance, but it has shown that supplements protect against tissue damage induced by exercise. Recent studies have reported that over 35% of the Spanish people eat diets low or very low quality, which is clearly shown that over 10% of Spaniards have poor intakes (\<2/3 IDR) riboflavin (men) , folate (women), vitamin C, vitamin A, vitamin D and vitamin E.
The overall objective of the study is to establish situations improving athletic performance and study the oxidative balance by manipulation of feeding patterns and consumption of nutritional supplements and investigate the involved mechanisms. The aim of this study is to establish scientific basis for design functional foods improving athletic performance and health and avoiding the negative consequences of the oxidative damage induced by overexertion.
University of the Balearic Islands is the lead sponsor of 39 studies on the registry; 5 are open to participants now.
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Exclusion Criteria:
1 liter/day of one experimental beverage (containing 0.2% olive oil + 0.6% DHA-S Martek) provides 1.14 g DHA/daily
Dietary Supplement: Docosahexaenoic acid diet supplementation
1 liter/day of placebo beverage (containing 0.8% olive oil)
Dietary Supplement: Placebo supplementation
Nine soccer players (19 and 22 years old) took 1 liter/day of one experimental beverage (containing 0.2% olive oil + 0.6% DHA-S Martek). Follow-up 8 weeks. At week 8 an acute exercise season was programed. One blood sample was taken in resting conditions at beginning of the nutritional intervention (week 0, baseline). Another two blood samples were taken at the end of the nutritional intervention, in resting (week 8, pre-exercise) and post-exercise conditions (week 8, post-exercise). Biochemical measures, oxidative damage markers on lipids, proteins and DNA, antioxidant capabilities (enzyme activities and quantity of enzyme protein and gene expression) and inflammatory markers were measured in serum, plasma, erythrocytes, neutrophils and peripheral blood mononuclear cells.
Six professionals soccer players (19 and 22 years old) took 1 liter/day of placebo beverage (containing 0.8% olive oil). Follow-up 8 weeks. At week 8 an acute exercise season was programed. One blood sample was taken in resting conditions at beginning of the nutritional intervention (week 0, baseline). Another two blood samples were taken at the end of the nutritional intervention, in resting (week 8, pre-exercise) and post-exercise conditions (week 8, post-exercise). Biochemical measures, oxidative damage markers on lipids, proteins and DNA, antioxidant capabilities (enzyme activities and quantity of enzyme protein and gene expression) and inflammatory markers were measured in serum, plasma, erythrocytes, neutrophils and peripheral blood mononuclear cells.
DHA incorporation in erythrocyte cellular membranes
Docosahexaenoic acid (DHA) is a conditionally essential fatty acid. Primary outcome: DHA incorporation in the erythrocyte cellular membranes by gas chromatography of soccer players during a training season
Time frame: Baseline, week 8
DHA on oxidative stress
Effects of DHA diet supplementation, the training season and acute exercise on oxidative and nitrosative damage markers and antioxidant enzyme activities, and protein levels in different blood fractions.
Time frame: Baseline, week-8 (pre- and post-exercise)
DHA on inflammation
Effects of DHA diet supplementation, the training season and acute exercise on inflammation markers.
Time frame: Baseline, week-8 (pre- and post-exercise)
This study is completed, as verified in Jun 2014. You cannot join it, but the record below documents what was studied.
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University of the Balearic Islands